Selection of Potent Isolates from a Population of<i> Alternaria Alternata</i>, a Leaf Spot Pathogen of Poplar

Abstract

Poplar, an important tree in the agri-silvicultural system, is propagated mainly through cuttings to maintain genetic purity. Monocultures of poplar clones are amenable to many diseases as they have a narrow genetic base. Pathogenic populations have variability in terms of pathogenicity and virulence which are governed by its genetic makeup. Mapping the variability and selection of potential pathogenic isolates for breeding disease resistance remains a challenge. During the survey conducted in poplar nurseries located at different geographical sites, altogether 72 isolates of Alternaria alternata, were collected from four commercial clones of P. deltoides. Three selection methods were attempted to select fifteen potent A. alternata isolates based on growth rate, sporulation and spore size (maximum length and maximum breadth). Initially, Rough Gauging Method which is simply based upon index of sum of the character's values and Equal Class Interval Method which depends upon the index of class interval scores were applied. To overcome the limitations of the above two methods, Unequal Class Interval Method was proposed based on Coefficient of Variation for each character assessed. The index was constructed using the geometric rather than arithmetic mean as the former normalizes the range, so that, no range dominates the scores assigned to the characters. The proposed method is recommended for the situations when the criterion variable depends upon various growth characters having inherent significant variation among each other.

Lifestyle-specific responses ofTrichodermaspp. in mycoparasitic confrontations and implications for biocontrol ofPopulusxcanescens

“…In addition to functioning as a bio-fungicide, numerous rhizosphere-competent Trichoderma spp. can form a close symbiosis with plant roots, producing soluble metabolites and volatile organic compounds (VOCs) with plant-performance stimulating activities conferring improved plant growth and induced resistance to abiotic stresses (Garnica-Vergara et al, 2016; Harman et al, 2004; Nawrocka & Małolepsza, 2013; Uniyal et al, 2018). Therefore, the application of Trichoderma spp.…”