Selection of Pru p 3 hypoallergenic peach and nectarine varieties

Jin, Jing; Gao, Ling; Zhao, Lan; Zhong, Gongxun; Li, Xiongwei; Xie, Hanbing; Ni, Junbei; Gan, Kexin; Wu, Shihua; Ye, Zhengwen; Jun, Luo; Cao, Ke; Ma, Ruijuan; Chen, Miaojin; Arús, Pere; Versteeg, Serge A.; Wang, Huiying; Mei-ling, Liu; Jia, Huijuan; Ree, Ronald van

doi:10.1111/all.14102

Cited by 5 publications

(12 citation statements)

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“…For example, nectarines such as "Zhong You 7," "Hu You 277/278," and "Nan Fang Jin Mi," have been found to contain low Pru p 3 but high Pru p 1. 19 This phenomenon is similar to Mal d 1 and Mal d 3 content in apple. 26 Considering fruit quality, it is noticeable that those considered to be of good quality (strong aroma, high sugar content, and nutritional value) are usually accompanied with high allergen levels, as in peach and apricot.…”

Section: Qpcr Ofsupporting

confidence: 68%

“…We have previously found that Pru p 3 content is related to ripening date, sugar content, and aroma, while this correlation was not identified in Pru p 1. 19 Our quantification data showed that most nectarines contained high levels of Pru p 1, some up to 5.97 μg/g.…”

Section: Qpcr Ofmentioning

confidence: 71%

“…In previous research we found that Pru p 3 is generally low in ancient and early-maturing red flesh peach varieties. 19 From an evolutionary point of view, Pru p 3 content was very low in the "Mao Tao" primary wild peaches and some local landraces (old varieties cultivated in a small area) of red-fleshed peaches, and higher in most modern peach varieties, especially late-ripening yellow-fleshed varieties. A similar phenomenon is also found in the quantification of Pru p 1.…”

Section: Qpcr Ofmentioning

confidence: 99%

“…-5 of 10 sELISA is commonly used in fruit allergen quantification, for orange, apple, and peach. 15,[17][18][19]25 This method is advantageous in that it reacts directly with allergens in food and is highly sensitive and accurate. Owing to the similar epitopes of Pru p 1.01 and Pru p 1.02, our method was developed using a monoclonal (A0-A7-G11) and two polyclonal antibodies (P1 and P2), able to effectively identify these two major isoforms to determine total Pru p 1 level in peach fruit.…”

Section: Qpcr Ofmentioning

confidence: 99%

“…LTP is stable, mainly concentrated in the peel and the levels vary greatly in different varieties, related to ripening, aroma, and sugar content. [17][18][19] The content of PR-10 proteins has been found to be lower than that of LTP in peach, and is usually affected by genotype, storage conditions and storage time. 14,20 A recent study selected Pru p 3 hypoallergenic varieties after measuring over 100 varieties using sensitive monoclonal antibody ELISA.…”

Section: Introductionmentioning

confidence: 99%

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Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties

Jin

Gan

Zhao

et al. 2021

Clinical & Translational All

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Background:Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods:To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme-linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction.Results: A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20-73.44 μg/g, fresh weight), being very low in the pulp (0.05-9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 contentThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Qpcr Ofsupporting

confidence: 68%

Section: Qpcr Ofmentioning

confidence: 71%

Section: Qpcr Ofmentioning

confidence: 99%