Selection of Storage Methods for Maintenance of Different Stock Cultures

manjana, Angshu; banjana, Anir; Arijitmajumdar, Dipjitdey; Tudu, Jayantabikashdey Nikhil

doi:10.20546/ijcmas.2016.510.115

Cited by 6 publications

(2 citation statements)

References 1 publication

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“…[27] For E. coli preservation, 0.5 ml of overnight incubated nutrient broth containing E. coli was added to sterile tubes containing 0.5 ml sterile glycerol, mixed well, and stored at -20°C, for 6 months. [9]…”

Section: Laboratory Diagnosismentioning

confidence: 99%

The Prevalence Of Uropathogenic Escherichia Coli Strains Among Outpatients With Urinary Tract Infection In Zakho City, Iraq

Ibrahim

Khalid²,

Mer³

2021

Al-Qadisiyah Journal of Pure Science

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This study involved the prevalence of uropathogenic Escherichia coli (UPEC) among outpatients of UTI attending three major hospitals in Zakho city. Four hundred urine samples were collected from patients of UTI of both sexes and different ages (≤ 1 year to over 50 years), during the period from July 2018 until January 2019. All urine samples were analyzed by conventional bacteriological method for the presence of Escherichia coli (E. coli), while molecular method was used for the presence of species-specific uidA gene in the isolated E. coli. Out of 400 samples, 141 (35.25%) were infected with UPEC from enrolled patients. The rate was higher in females than males (90.78% vs 9.22%), respectively. In both sexes, the age group 41-50 years in both sexes showed the highest rate (46.67%) of infection, and statistically this rate of infection was significant (p< 0.013) among both sexes and various age groups. Furthermore, in all ages, married patients showed slightly higher prevalence than un-married one (38% vs 32.5%), but this difference was statistically non-significant (p>0.05%). The rate of UTI was higher among urban inhabitants (40.56%) than others. During the months of the year, the peak (90.48%) in both sexes was during December while the lowest rates (13.64%) was during January.

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Section: Laboratory Diagnosismentioning

confidence: 99%

The Prevalence Of Uropathogenic Escherichia Coli Strains Among Outpatients With Urinary Tract Infection In Zakho City, Iraq

Ibrahim

Khalid²,

Mer³

2021

Al-Qadisiyah Journal of Pure Science

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“…After isolation and purification, some PSB isolates did not exhibit the activity (solubility of Ca3(PO4)2) which may have been due to an inappropriate storage medium (Angshumanjana et al, 2016). The repeated subcultures decreased the activity of bacterial strains antagonizing Staphylococcus aureus was mentioned by Neha et al (2019).…”

Section: Effects Of Psb Isolates On Seeds In Plate Assaymentioning

confidence: 99%

Plant growth promoting characterization of indigenous phosphate solubilizing rhizobacteria and effects on germination of some crops in Vietnam

NGUYEN

NGUYEN²,

NGUYEN

2023

Not Bot Horti Agrobo

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Inorganic Phosphate Solubilizing Bacteria (PSB) are widely present in nature and have been successfully applied in fields in many countries. However, researches on indigenous PSB are still very limited in Vietnam. The objective of this study was to isolate and evaluate the growth promoting characteristics and effects on germination of seed of indigenous PSB for fertilizer production from PSB. Thirteen isolates of indigenous PSB were collected using selective isolation medium containing Ca3(PO4)2, of which eight added the ability to dissolve AlPO4 and five isolates added the ability to dissolve FePO4. Initial qualitative tests indicated that all thirteen PSB isolates were incapable of HCN and lipase production while other growth promoting activities including amylase, caseinase, cellulase, chitinase, pectinase, indole acetic acid, K solubility, Zn solubility, and N fixation varied according to PSB isolate. The collected PSB isolates had no effect on seed germination rate, root length, and hypocotyl length of plantules of soybean, rice, maize, cucumber, tomato, and chili by plate assay. This study had shown that PSB was also common in the rhizosphere of various crops in Vietnam. Therefore, isolation to enrich the indigenous PSB collection was essential for the screening of suitable PSB strains for subsequent fertilizer production.

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Consortia of Specific Bacteria in Callyspongia subarmigera (Ridley, 1884) and their Exocellular Activity against Clinical and Fish Pathogens

2018

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Selection of Storage Methods for Maintenance of Different Stock Cultures

Cited by 6 publications

References 1 publication

The Prevalence Of Uropathogenic Escherichia Coli Strains Among Outpatients With Urinary Tract Infection In Zakho City, Iraq

The Prevalence Of Uropathogenic Escherichia Coli Strains Among Outpatients With Urinary Tract Infection In Zakho City, Iraq

Plant growth promoting characterization of indigenous phosphate solubilizing rhizobacteria and effects on germination of some crops in Vietnam

Consortia of Specific Bacteria in Callyspongia subarmigera (Ridley, 1884) and their Exocellular Activity against Clinical and Fish Pathogens

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