Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merril] plants at a high frequency

Aragão, F. J. L.; Sarokin, Laura; Vianna, Giovanni R.; Rech, E. L.

doi:10.1007/s001220051441

Cited by 140 publications

(128 citation statements)

References 15 publications

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“…P3183458 and P3178672) construct. A rd29A:AtDREB1A construct positive soybean line, P58, was obtained by particle-bombardment transformation according to Aragão et al (2000) and Rech et al (2008), and it was submitted to drought in a greenhouse experiment.…”

Section: Plant Material Growth Conditions and Statistical Designmentioning

confidence: 99%

Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance

Polizel¹,

Medri²,

Nakashima³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydrationresponsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each treatment performed in triplicate. Seeds from the non-transformed soybean cultivar BR16 and from the genetically modified soybean P58 line (T 2 generation) were grown at 15% gravimetric humidity for 31 days. To induce water deficit, the humidity was reduced to 5% gravimetric humidity (moderate stress) for 29 days and then to 2.5% gravimetric humidity (severe stress). AtDREB1A gene expression was higher in the genetically modified P58 plants during water deficit, demonstrating transgene stability in T 2 generations and induction of the rd29A promoter. Drought-response genes, including GmPI-PLC, GmSTP, GmGRP, and GmLEA14, were highly expressed in plants submitted to severe stress. Genetically modified plants had higher stomatal conductance and consequently higher photosynthetic and transpiration rates. In addition, they had more chlorophyll. Overexpression of AtDREB1A may contribute to a decrease in leaf thickness; however, a thicker abaxial epidermis was observed. Overexpression of AtDREB1A in soybean appears to enhance drought tolerance.

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Section: Plant Material Growth Conditions and Statistical Designmentioning

confidence: 99%

Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance

Polizel¹,

Medri²,

Nakashima³

et al. 2011

Genet. Mol. Res.

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“…Embryos of cultivar BR 16 were co-transformed with two expression vectors, 35S::AtAREB1∆QT (Fujita et al, 2005) and 35S::ahas, by particle bombardment according to the method described by Aragão et al (2000) and Rech et al (2008). The transformation method, selection, and regeneration procedures were described in Barbosa et al (2013).…”

Section: Soybean Transformation and Polymerase Chain Reaction (Pcr) Amentioning

confidence: 99%

Overexpression of the activated form of the AtAREB1 gene (AtAREB1ΔQT) improves soybean responses to water deficit

Leite¹,

Barbosa²,

Marin³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter 6273 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6272-6286 (2014) Water deficit response in GM soybeans with AtAREB1∆QT region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AREB1∆QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1∆QT show improved drought tolerance. In our studies, soybean plants overexpressing AREB1∆QT were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of AREB1∆QT soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stress onset. The difference in the degree of wilting and percentage of survival between the 35S-AREB1∆QT and wildtype plants may also be related to the regulation of genes that protect against dehydration because metabolic impairment of photosynthesis, deduced by an increasing internal CO 2 concentration, was not observed in the transgenic plants.

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“…The particle bombardment transformation method was first used in soybean in 1988 by McCabe et al, who successfully transformed immature seed meristem [6] and was followed by the transformation of somatic embryonic tissue [7], and apical meristem [8]. Transformation of somatic embryos has been the most successful method and is induced from immature cotyledons cultured on medium containing moderately high concentrations of an auxin.…”

Section: Soybean Transformationmentioning

confidence: 99%

Advancements in Transgenic Soy: From Field to Bedside

Hudson¹,

Lambirth²,

Bost³

et al. 2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merril] plants at a high frequency

Cited by 140 publications

References 15 publications

Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance

Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance

Overexpression of the activated form of the AtAREB1 gene (AtAREB1ΔQT) improves soybean responses to water deficit

Advancements in Transgenic Soy: From Field to Bedside

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