Selection with melphalan or paclitaxel (Taxol) yields variants with different patterns of multidrug resistance, integrin expression and in vitro invasiveness

Enhanced in vitro invasiveness and drug resistance with altered gene expression patterns in a human lung carcinoma cell line after pulse selection with anticancer drugs

“…We previously reported that melphalan selection induced a human nasal carcinoma cell line, RPMI-2650, to become more invasive in vitro, whereas paclitaxel could not. 12 Here, we expanded that study using a different cell line (in case the initial results had any element of cell specificity) to investigate 10 other commonly used chemotherapeutic drugs (to investigate if such effects are specific to melphalan or may occur in response to other drugs).…”

“…10 We have previously demonstrated that exposure of the human nasal carcinoma cell line RPMI-2650 to melphalan, an alkylating agent, can result in not only an MDR phenotype but also enhanced cell invasion in vitro, whereas paclitaxel (Taxol) exposure resulted in MDR without increasing cell invasiveness. 12 To further explore the association between drug resistance and cancer invasion/metastasis and, in particular, whether such a phenomenon can be observed in a different kind of human tumour and with different drugs, we studied this phenomenon in tumour cells from a different organ of origin, using a broad range of chemotherapeutic drugs. We pulse-selected the poorly differentiated human lung squamous carcinoma cell line DLKP with 10 commonly used drugs, including VP-16, VCR, TXT, MITOX, the 2 antimetabolites 5-FU and MTX and the 4 alkylating agents CCNU, BCNU, CPT and CHLO, and extensively characterised the resulting cell populations.…”

Olfactomedin III expression contributes to anoikis-resistance in clonal variants of a human lung squamous carcinoma cell line

“…MCF-7, SKBR3 and RPMI-2650 were obtained from the ATCC. RPMI-2650tx was previously obtained by continuous exposure to taxol [10]. MCF-7 and SKBR3 were maintained in RPMI-1640 supplemented with 10% FBS and 1% L-glutamine.…”

A new superinvasive in vitro phenotype induced by selection of human breast carcinoma cells with the chemotherapeutic drugs paclitaxel and doxorubicin

“…Invasion and metastasis are intimately involved in the pathology of cancer, but the biological and molecular natures of these phenomena remain incompletely understood. A possible association exists between multiple drug resistance and invasive potential in carcinoma cells (Liang et al, 2001(Liang et al, , 2002.The majority of previous studies focus on the direct effect of drug exposure on the invasive potential (invasion in the presence of the drug), as opposed to the long-term effects of drug treatment on the invasive phenotype of cell, coupled with emerging drug resistance. While a few reports indicate that paclitaxel exposure increases motility and invasion (Welch et al, 1989;Silbergeld et al, 1995), others indicate an inhibitory effect on invasion (Westerlund et al, 1997;Sgadari et al, 2000).…”

“…Invasion and metastasis are intimately involved in the pathology of cancer, but the biological and molecular natures of these phenomena remain incompletely understood. A possible association exists between multiple drug resistance and invasive potential in carcinoma cells (Liang et al, 2001(Liang et al, , 2002.…”