Selective activity of polyene macrolides produced by genetically modified Streptomyces on Trypanosoma cruzi

Rolón, Míriam; Seco, Elena M.; Vega, Celeste; Nogal, Juan José; Escario, José Antonio; Gómez‐Barrio, Alicia; Malpartida, Francisco

doi:10.1016/j.ijantimicag.2006.02.025

Cited by 59 publications

(34 citation statements)

References 29 publications

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“…The plate was incubated at 24°C for 72h. After this, 20µL of a 2mM resazurin solution was added to each well (25) . The plates were incubated for 4h, and the reduction of resazurin was determined by readings obtained from a fluorimeter (excitation wavelength of 485nm and emission wavelength of 590nm).…”

Section: Evaluation Of the Leishmanicidal Potential Of The Crude Extrmentioning

confidence: 99%

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Correia

Lima

Oliveira

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Malaria and leishmaniasis are prevalent in tropical regions, which have environmental characteristics that are highly favorable to protozoa and vectors of these diseases; the transmission of these infections in sub-tropical regions, although recognized, represents only a small fraction of cases. Plants are constantly being used in the search for and acquisition of new drugs, and many compounds derived from them have been used to combat various diseases. In this study, we evaluated the action of the dichloromethanolic extract of Myrciaria dubia leaves against the protozoa Plasmodium falciparum, Leishmania amazonensis, Leishmania braziliensis, and Leishmania chagasi through bioassays. Methods: The extract from M. dubia was tested for its anti-P. falciparum activity in an anti-histidine-rich protein II immunosorbent assay. The antileishmanial assays were performed using the resazurin method, while cytotoxicity against human hepatoma (HepG2) strain was determined using the colorimetric MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide] method. Results: The M. dubia extract presented a half-maximal inhibitory concentration equal to 2.35 (1.05)μg/mL for P. falciparum, 190.73 (6.41) μg/mL for L. amazonensis, and greater than equal to 200µg/mL for L. chagasi and L. braziliensis strains. The cytotoxic concentration for 50% of the cells was above 500μg/mL for HepG2, indicating no toxicity and greater selectivity against parasites. Conclusions:The results obtained indicate the presence of antiplasmodial and leishmanicidal bioactive compounds in the dichloromethanolic extracts of M. dubia leaves, and point towards future studies to elucidate the mechanism of action for each physiological effect.

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Section: Evaluation Of the Leishmanicidal Potential Of The Crude Extrmentioning

confidence: 99%

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Correia

Lima

Oliveira

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…The cultures were maintained at 37°C in a humid atmosphere with 5% CO 2 . Cell viability was assessed by the resazurin reduction assay (Rolón et al, 2006) with cells grown in 96-well microplates at a final concentration of 3 Â 10 4 cells/well. After the culture medium was removed, 20 lL of 2 mM resazurin was added to each well with a final volume of 200 lL.…”

Section: Determination Of Cytotoxicitymentioning

confidence: 99%

Analysis of bioactivities and chemical composition of Ziziphus joazeiro Mart. using HPLC–DAD

et al. 2015

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The aim of this study was to evaluate the chemical profile and antioxidant, antimicrobial and antiparasitic activities of the hydroalcoholic extract of the leaves of Ziziphus joazeiro Mart. (HELZJ). The antioxidant DPPH and FRAP assays and chemical profile were determined by colorimetric methods and HPLC/DAD. The antiparasitic, antibiotic and antibiotic-modifying activity were evaluated by microdilution assays. The HPLC-DAD assay showed the presence of mostly tannins and flavonoids, such as caffeic acid and quercetin. The levels of polyphenols and flavonoids were 183.136 mg/g extract and 7.37 mg/g extract, respectively. DPPH and FRAP showed low antioxidant activity for the extract. The antibacterial and antifungal activities were not of clinical relevance, showing MIC>1024 μg/mL. However, synergism was observed between HELZJ and the antibiotics amikacin and gentamicin, which resulted in decreased bacterial drug resistance. EHFZJ showed low toxicity in fibroblasts in vitro, while antiparasitic results against Trypnosoma cruzi, Leishmania braziliensis and Leishmania infantum were not clinically relevant. Thus, our results indicate that Z. joazeiro Mart. (HELZJ) could be a source of plant-derived natural products that could lead to the development of promising new antibiotic compounds for infectious diseases.

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“…The activity was evaluated with chlorophenol red-␤-dgalactopyranoside (CPRG) by a modified colorimetric method described previously (Buckner et al, 1996;Rolon et al, 2006).…”

Section: Anti-amastigote Activitymentioning

confidence: 99%

“…The bioassay was performed as previously described by Rolon (Rolon et al, 2006). Briefly, NCTC 929 cells were plated in 96-well microtitre plates at 3 × 10 4 cells/well in 100 L of growth medium.…”

Section: Cytotoxic Activitymentioning

confidence: 99%

Zanthoxylum chiloperone leaves extract: First sustainable Chagas disease treatment

Ferreira

Cebrián‐Torrejón

Corrales

et al. 2011

Journal of Ethnopharmacology

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Selective activity of polyene macrolides produced by genetically modified Streptomyces on Trypanosoma cruzi

Cited by 59 publications

References 29 publications

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract

Analysis of bioactivities and chemical composition of Ziziphus joazeiro Mart. using HPLC–DAD

Zanthoxylum chiloperone leaves extract: First sustainable Chagas disease treatment

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