Selective and rapid solubilization of the microbial membrane enzyme aldehyde dehydrogenase

Abstract

An improved solubilization procedure for the membrane-bound quinoprotein aldehyde dehydrogenase from Acetobacter rancens CCM 1774 was established. After the first solubilization of membrane enzymes by Brij 35 which provided important extraction of membrane proteins other than aldehyde dehydrogenase, the application of Trition X-100 resulted in an almost 20-fold purification of quinoprotein aldehyde dehydrogenase. The optimal solubilization was closely connected with definite detergent/protein ratios.

Facile solvent free synthesis of polymerised sucrose functionalised polyoxyethylene (23) lauryl ether by microwave irradiation

“…8,9 Polyoxyethylene (23) lauryl ether or Brij R 35 is an extensively studied nonionic surfactant and finds many applications including dissolution of polymers and extraction of proteins from membranes. 10,11 The microwave irradiation (MW) technique for chemical syntheses has been popular in recent years and there are many reports of the preparation of new materials using this technique. 12- 15 It was found that the rate of reactions and yield of product for the reactions carried out by MW irradiation is superior to the conventional heating techniques.…”