Selective and sensitive detection of dopamine in the presence of ascorb ic acid and uric acid at a Sonogel - Carbon L - Histidine modified electrode

Abstract

Design and application of sensitive and selective electrochemical sensors for the determination of biologically important substances have gained considerable interest in recent years [1][2][3][4][5]. Dopamine (DA) is an important neurotransmitter molecule of catecholamines that is widely distributed in the mammalian central nervous system for message transfer. It plays a very important role in the function of central nervous, renal, hormonal, and cardio vascular systems [6]. Extreme abnormalities of DA levels can lead to brain disorders such as parkinsonism and schizophrenia [7,8]. Therefore, developing simple and rapid methods for the sensitive determination of DA in routine analysis is always a challenge [9,10]. In this context, electrochemical techniques have generated considerable attention, in recent years as good tools for the study of electrochemical mechanisms and for the detection and quantification of biological substances, in particular DA, AA and UA. However, AA and UA oxidize at similar potentials with DA and the result is an overlap of their voltammetric responses when using conventional electrodes. This overlap of potentials has an adverse effect on electrode selectivity and reproducibility [11,12]. In literature, there are many papers reporting the manufacture process and analytical applications involving Sonogels. The Sonogel-Carbon materials were patented and described first by Hidalgo-Hidalgo de Cisneros et al [13,14]. Recently a new type of graphite-based sol-gel electrode has been developed, the sonogel-carbon electrode, which was obtained using high-energy ultrasounds. In general, classical procedures for the synthesis of acid catalysed sol-gel-based electrode materials include the addition of an alcoholic solvent to the initial precursor mixture to make it homogenous. This is followed by the employment of an ultrasound bath for several minutes to promote the hydrolysis. in the other hand, by means of sonocatalysis, high-energy ultrasounds is applied directly to the precursors, and ultrasonic cavitation, sol-gel reactions occur in a unique environnement, leading to gels AbstractThe Sonogel-Carbon electrode is a special class of sol-gel electrode that exhibits efficient mechanical and electrical properties. In this study, SonogelCarbon modified with L-Histidine was used to prepare a novel electrochemical sensor. This new electrode showed excellent electrocatalytic behavior towards the oxidation of dopamine (DA) in PBS and human serum. The proposed sensor was applied to the simultaneous determination of dopamine, ascorbic acid (AA) and uric acid (UA) in pH 7.4 PBS and excellent results were obtained. A lower detection limit of 1×10 −7 M was achieved. This work, illustrates, for first time, the use of L-histidine Sonogel-Carbon electrode as a simple and low-cost tool for the selective and sensitive determination of DA in the presence of AA and UA.

Recent Advances in Electrochemical and Optical Sensing of Dopamine

“…With regard to selectivity, after adding 10 µM of DA the current change of 8 nA was obtained; while after the same concentrations of glucose and AA were added, no significant change in the signal was observed. Atyah et al (2017) prepared a novel, simple, low-cost EC sensor to detect DA in the presence of AA and UA with high sensitivity and selectivity using a sonogel-carbon (SNGC) electrode which was modified with L-histidine (L-His SNGC) [113]. The electrocatalytic behavior of this innovative electrode towards DA oxidation was great in human serum and phosphate-buffered saline (PBS).…”

Mesoporous chitosan based conformable and resorbable biostrip for dopamine detection

“…When DA redox peaks were compared with that from a mixture of DA and UA solutions (Fig. 5b), later revealed two distinctly separated anodic peaks of significantly enhanced current intensity (Atyah et al 2017) and was attributed to the increased concentration of DA-UA solution (Nayak et al 2016). This was confirmed by the disappearance of AA peaks when similar experiments were conducted with DA and AA mixture solutions (Fig.…”