Selective and sensitive determination of protoberberines by capillary electrophoresis coupled with molecularly imprinted microextraction

Abstract

In this work, we developed a novel molecularly imprinted solid-phase microextraction with capillary electrophoresis method for the selective extraction and determination of protoberberines in complicated samples. The imprinted monolith was prepared in a micropipette tip-based device by using acrylamide as the functional monomer, ethyleneglyoldimethacrylate as the cross-linker and dimethylsulfoxide as the porogen, and exhibited an imprinting factor of 2.41 to berberine, 2.36 to palmatine and 2.38 to jatrorrhizine. Good capillary electrophoresis separation was achieved by using 20 mM phosphate buffer at pH 7 as running buffer with the addition of organic modifier of 10% methanol. Parameters such as sample pH value, sample flow rate and sample volume were investigated for imprinted monolith-based solid-phase microextraction. An imprinted solid-phase microextraction with capillary electrophoresis method was developed, the method showed a wide linear range (0.3-50 μg/mL), good linearity (R ≥ 0.9947) and good reproducibility (relative standard deviations ≤ 0.73%), the limit of detection was as low as 0.1 μg/mL, which was lower than some reported methods based on capillary electrophoresis for protoberberines. The method has been applied for determination of three common protoberberines in Cortex Phellodendri Chinensis, by using a molecularly imprinted monolith as the selective sorbent, most of the matrices in the Cortex Phellodendri Chinensis sample were removed and three protoberberines were selectively enriched and well determined.

Capillary electrophoresis in phytochemical analysis (2014–2017)

“…Traditional methods such as Soxhlet extraction, reflux, ultrasonic and liquid‐liquid extraction were usually used for phytochemical extraction from various samples. Besides, pressurized liquid extraction (PLE) or accelerated solvent extraction (ASE) , MAE , SPE , matrix solid‐phase dispersion (MSPD) and SPME were also gained much attentions.…”

A LC‐MS/MS method for simultaneous determination of seven alkaloids in rat plasma after oral administration of Phellodendri chinensis cortex extract and its application to a pharmacokinetic study

“…With regard to Phellodendri chinensis cortex , the alkaloids, polysaccharides , flavonoids , phytosterols, coumarins , and triterpenes are the major ingredients. A large amount of pharmacological reports proved that alkaloid components including phellodendrine, jatrorrhizine, palmatine, berberine, magnoflorine, columbamine, protopine, and tetrahydropalmatine were the main active components of Phellodendri chinensis cortex . Modern pharmacological experiments suggested that Phellodendri chinensis cortex possessed various pharmacological actions , which has been widely prescribed to remove damp heat, eliminate dampness, counteract toxicity, purge pathogenic fire, relieve consumptive fever, diarrhea and other syndromes .…”

Tailor‐made ion‐imprinted polymer based on functionalized graphene oxide for the preconcentration and determination of trace copper in food samples

“…Molecularly imprinted polymers (MIP), with virtues of high stability, high selectivity, and ease of synthesis, have been widely applied in selective enrichment of target compounds in complex matrix . Based on the idea of the molecular imprinting technique, ion‐imprinted polymers (IIP) have attracted much attention as selective sorbents for enrichment of metal ions before analysis .…”