Selective Cell Attachment to a Biomimetic Polymer Surface through the Recognition of Cell-Surface Tags

Abstract

Reactive phosphorylcholine polymers, which can recognize biosynthetic cell-surface tags, were synthesized to control cell attachment. Human promyelocytic leukemia cells (HL-60) with unnatural carbohydrates as cell-surface tags were harvested by treatment with N-levulinoylmannosamine (ManLev). The attachment of ManLev-treated HL-60 cells to 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers with hydrazide groups was studied. HL-60 cells, which are nonadhesive, did not attach to any polymer surface without ManLev treatment. In contrast, ManLev-treated HL-60 cells attached to a poly[MPC-co-n-butyl methacrylate (BMA)-co-methacryloyl hydrazide (MH)] (PMBH) surface following 15 min of incubation. The cells that attached to the PMBH surface retained their native morphology and viability for 24 h of incubation. On the other hand, approximately half of the HL-60 cells that attached to the poly(BMA-co-MH) (PBH) surface died. These results suggest that MH units in the polymer act as anchors for cell attachment and MPC units help to preserve cell viability on a polymer surface. The coculture of ManLev-treated HL-60 and fluorescence-stained human uterine cervical cancer cells (HeLa) was carried out on polymer surfaces. ManLev-treated HL-60 cells specifically attached to the PMBH surface. In contrast, both HL-60 and HeLa cells were observed on the PBH surface. The control of cellular interactions with synthetic polymers may be useful for the future development of cell-integrated biosensors and biomedical devices.

Adhesion and Proliferation of Human Periodontal Ligament Cells on Poly(2-methoxyethyl acrylate)

“…PDL cells hardly adhered and proliferated on PMPC, which was consistent with the findings of a previous study by Iwasaki et al, who reported that adhesion of human promyelocytic leukemia cells and human uterine cervical cancer cells was completely suppressed on PMPC [36]. We previously reported that, we previously reported that PMEA has excellent biocompatibility with human blood cells [15].…”

Preparation and characterization of fibroin/hyaluronic acid composite scaffold

“…Some investigators have sought to take advantage of the properties of HA by combining HA with other biomaterials, including collagen [23,24], gelatin [25][26][27] and chitosan [28][29][30], to produce scaffolds or membranes used for tissue engineering. In this study, we hypothesized that HA could provide superior effects on the formation, structural and biological properties of fibroin-based scaffolds.…”

Chemical Biology of Cell Surface Oligosaccharides

“…In other cases, direct chemoselective ligation of fluorescent molecules [49,56] or contrast agents [57] has been reported. Probes have also included therapeutic agents such as toxic proteins [50] and small-molecule drugs [58], additional sugar epitopes [51], quantum dots [54], adenoviruses used for gene delivery [59], as well as polymers [60] and functionalized materials intended to facilitate cell adhesion and serve as a growth substrate [54,61].…”

“…In a second example of using exogenous precursors to influence bacterial carbohydrate biosynthesis, the Nishimura laboratory synthesized UDP-MurNAc pentapeptide derivatives that were metabolically incorporated into cell-wall components of Lactobacilli plantarum [103], thereby changing the adhesive properties of these bacteria [104]. Both strategies offer a way to identify the pathogen to the immune system for eradication, or to adhesive constructs for removal from the body [60,61].…”

“…Iwasaki and colleagues implemented this strategy by treating HL-60 neutrophils with ManNLev and culturing these ketone-expressing cells on polymer surfaces functionalized with hydrazides. The normally nonadhesive cells selectively attached to the modified polymer surfaces [60]. These studies, although directed at the separation of cancer cells, lay a scientific foundation for the use of MOE analogs for tissue engineering where cell attachment to biopolymeric materials used as scaffolds is a critical first step in the formation of artificial tissues.…”