Selective enrichment of zein gene of maize from cereal products using magnetic support having pyrrolidinyl peptide nucleic acid probe

Khadsai, Sudarat; Seeja, Noppadol; Rutnakornpituk, Metha; Vilaivan, Tirayut; Nakkuntod, Maliwan; Suwankitti, Waroon; Kielar, Filip; Rutnakornpituk, Boonjira

doi:10.1016/j.foodchem.2020.127812

Cited by 6 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The surface modification of MNPs utilized various functional groups, which can immobilize different biological ligands in the analyte [ 59 ]. Commonly used MNPs include histidine-tagged MNPs [ 91 ], diethylaminoethylcellulose MNPs [ 92 ], heparin MNPs [ 93 ], flag-labeled MNPs, antibody MNPs (Protein A/Protein G/Protein L) [ 94 , 95 ], Strep-tag II MNPs [ 96 ], adapter MNPs [ 97 ], and solid-phase peptide MNPs [ 98 , 99 ], primarily for the purpose of separating and purifying the analyte.…”

Section: Applications Of Mnps In Biomedical Fieldsmentioning

confidence: 99%

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Hong,

Wang,

Zheng

et al. 2024

Materials

View full text Add to dashboard Cite

Magnetic nanoparticles (MNPs) have found extensive application in the biomedical domain due to their enhanced biocompatibility, minimal toxicity, and strong magnetic responsiveness. MNPs exhibit great potential as nanomaterials in various biomedical applications, including disease detection and cancer therapy. Typically, MNPs consist of a magnetic core surrounded by surface modification coatings, such as inorganic materials, organic molecules, and polymers, forming a nucleoshell structure that mitigates nanoparticle agglomeration and enhances targeting capabilities. Consequently, MNPs exhibit magnetic responsiveness in vivo for transportation and therapeutic effects, such as enhancing medical imaging resolution and localized heating at the site of injury. MNPs are utilized for specimen purification through targeted binding and magnetic separation in vitro, thereby optimizing efficiency and expediting the process. This review delves into the distinctive functional characteristics of MNPs as well as the diverse bioactive molecules employed in their surface coatings and their corresponding functionalities. Additionally, the advancement of MNPs in various applications is outlined. Additionally, we discuss the advancements of magnetic nanoparticles in medical imaging, disease treatment, and in vitro assays, and we anticipate the future development prospects and obstacles in this field. The objective is to furnish readers with a thorough comprehension of the recent practical utilization of MNPs in biomedical disciplines.

show abstract

Section: Applications Of Mnps In Biomedical Fieldsmentioning

confidence: 99%

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Hong,

Wang,

Zheng

et al. 2024

Materials

View full text Add to dashboard Cite

show abstract

“…In addition, the polymers coating on the particle surface can also serve as a platform for functionalization with bio-entities, e.g. creatinine [40], peptide and DNA [41][42][43]. For example, positively charged polymers such as poly(dimethylamino)ethyl methacrylate (PDMAEMA) and PDEAEMA grafted from MNP surface can be adsorbed with negatively charged DNA via electrostatic interaction and used for DNA extraction/isolation [44].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticles Coated with Cationic Polymer and Label-Free Peptide Aptamer for Selective Adsorption with Bisphenol A

Tummachote

Rutnakornpituk²,

Rutnakornpituk³

2023

Trends Sci

View full text Add to dashboard Cite

Magnetite nanoparticles coated with quaternized poly(diethylamino)ethyl methacrylate (PDEAEMA) (PQDEA@MNP) and functionalized with bisphenol A (BPA)-specific peptide aptamer (CKSLENSYC) were synthesized and used as nano-adsorbents for selective adsorption with BPA. The polymer coated on the particles provided their good stability and dispersibility in water, and the aptamer provided selective adsorption with BPA targets. Fourier transformed infrared spectrophotometry (FTIR), thermogravimetric analysis (TGA) and vibrating sample magnetometry (VSM) techniques confirmed the presence of the polymer on the particle surface. From transmission electron microscopy (TEM) study, the particle size was ca.6 - 10 nm in diameter with some nanoclustering (30 - 60 nm in diameter of the nanoclusters). The selective adsorption of BPA on the particles was predominant over the non-specific adsorption when performed in PBS buffer (pH 10) in the presence of NaCl and tween-20. The nano-adsorbents had a good linearity range for the detection of BPA between 0.04 and 0.20 ppm with limit of detection (LOD) of 0.0145 ppm. They retained good BPA adsorption efficiency even when other interfering molecules were present. This simple measurement with the use of these nano-adsorbents without molecular labelling might be promising solid supports for use in various applications with good tolerance towards interfering molecules. HIGHLIGHTS Magnetic nanoparticle was coated with cationic polymer and BPA-specific aptamer This label-free peptide aptamer allowed for the selective capture of BPA targets It showed high selectivity to BPA capture and good tolerance to interfering molecules GRAPHICAL ABSTRACT

show abstract