Selective Extraction of Embelin from Embelia ribes by Hydrotropes

Latha, C.

doi:10.1080/01496390600957207

Cited by 13 publications

(6 citation statements)

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“…Embelin, a naturally occurring quinonoid compound, is found to be the major constituent of Embelia ribes. 19 Embelin, is the anti-cancer compound, so the plant exhibited the potent cytotoxicity against cancer cell lines. Embelin is reported to decrease tumor size and inhibit the increase in activity of serum enzymes, viz.…”

Section: Resultsmentioning

confidence: 99%

In-vitro Cytotoxicity Studies on Methanolic Leaf Extract of Embelia Ribes Burm F - an Important Traditional Medicinal Plant of Kerala

Raj¹

2016

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Abbreviations: U87, uppsala 87 (brain cancer cell line); Hep G2, liver hepatocellular carcinoma (human liver cancer cell line); MCF 7, michigan cancer foundation-7 (breast cancer cell line); E. ribes, embelia ribes; Gm, gram; Ml, milliliter; µg, micro gram; µl, microlitre ; °C, degree celsius; Rpm, revolutions per minute; DMSO, dimethyl sulfoxide; No, number; %, percent; U/ml, unit per milliliter; FBS, fetal bovine serum; pH, potential of hydrogen; MTT, 3-(4,5-dimethylthiazol-2-yl): 2,5-diphenyl tetrazolium bromide; A570, absorbance at 570nm; M, molar concentration; HCl, hydrochloric acid; CO 2 , carbondioxide; COLO-205, colon cancer cell lines; K562, human leukaemic cells; DLA, dalton's lymphoma ascites cells; BIR, baculovirus iap repeat; XIAP, x-linked inhibitor of apoptosis protein; COLO, 205 -colon cancer cell lines; LC, lethal concentration IntroductionCancer is the leading killing disease after the cardiovascular disorders. There is no proper medicine for controlling the growth of the cancer cells. There has long been standing interest in the identification of natural products for the treatment of various diseases for thousands of years. Natural products possess immense pharmacological significance in the development of drugs including cancer.1-3 The majority plant derived phytoconstituents, such as paclitaxel, etoposide, camptothecin, vinca alkaloids, indole alkaloids, podophyllotoxin derivatives, etoposide and teniposide, currently used in clinical cancer chemotherapy. The efficacy of chemotherapy, radiotherapy, hormonal therapy, or surgery, which are mainly used for the treatment of cancer, are well-known for side effects; 4 hence, the identification of novel natural products that possess better effectiveness against cancer, but less harmful effects have become desirable, 5 and therefore, natural products are continuously being explored worldwide.E. ribes is widely used as traditional herbal medicine in India. The plant is a climber with slender branches and long internodes. The leaves are elliptic, broad and covered with minute glands. The flowers are small, white racemes arranged in panicle inflorescence at the end of the branches. The fruits are berries, round, red to black color and tipped with style. 6 In Indian system of medicine 'Ayurveda', the plant is popularly known as Vidanga or Bashmak or Krimigna (Sanskrit); Baberangor Wawrung (Hindi); Vayuvilanga (Kannada) and it is used as one of the adjuvant in most of the drug preparations. The whole plant is used in the treatment of anti-inflammatory to relive rheumatism and fever. 7 The fruit is bitter in taste, good appetizer, cures tumors, ascites, bronchitis, jaundice and mental disorders. 8Seeds are used as antibiotic, anthelmintic, anti-tuberculosis, alterative and stimulative.9 Leaves are astringent, demulcent, depurative and useful in pruritus, sore throat, and ulcers of mouth, indolecent, skin diseases and leprosy.10 All the parts of this plant have the enormous medicinal properties. So the present study framed to investigate the in vitro cyto...

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Section: Resultsmentioning

confidence: 99%

In-vitro Cytotoxicity Studies on Methanolic Leaf Extract of Embelia Ribes Burm F - an Important Traditional Medicinal Plant of Kerala

Raj¹

2016

ACP

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“…This phenomenon, termed hydrotropy, can be an industrially attractive technique because solubility is much higher than with conventional methods (Maheshwari et al, 2006;Evstigneev et al, 2006;Shikha Agrawal et al, 2004;Dandekar et al, 2008). Easy recovery of dissolved solute and possible reuse of hydrotrope solution makes this method attractive at industrial levels (Koparkar and Gaikar, 2004;McKee, 1946;Badwan et al, 1982;Latha, 2006). Certain properties, such as solvent character being independent of pH, absence of fire hazards, absence of emulsification, and inexpensive aqueous phase, make this technique advantageous and superior to other solubilization methods.…”

Section: Introductionmentioning

confidence: 93%

Enhancement of Solubility and Mass-Transfer Coefficient of 1,2-Dihydroxy-9,10-Anthraquinone (Alizarin) Through Hydrotropy

Prakash¹,

Kumar

Gandhi

2009

Chemical Engineering Communications

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The effect of hydrotropes potassium p-toluene sulfonate (KPTS), citric acid, and nicotinamide on the solubility and mass-transfer coefficient of 1,2-dihydroxy-9,10-anthraquinone (alizarin) was studied. Solubility studies were carried out under a wide range of hydrotrope concentrations (0 to 3.0 mol Á L À1 ) and different system temperatures (303 to 333 K). It was observed that the solubility and mass-transfer coefficient of alizarin increases with an increase in hydrotrope concentration and system temperature. The maximum enhancement factor, the ratio of the value of solubility in the presence and absence of a hydrotrope, was determined for all experiments under study. The effectivity of hydrotropes was measured by the determination of the Setschenow constant, K s . The order of effectiveness of various hydrotropes based on K s values is potassium p-toluene sulfonate > citric acid > nicotinamide.

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“…(Rao and Venkateswarlu, 1961). Among them, embelin is considered one of the major bioactive constituents and marker compounds in E. ribes fruits (Chauhan et al ., 1999; Chitra et al ., 2003a; Latha., 2006). Embelin (3‐undecyl 2,5‐dihydroxy,1,4‐benzoquinone) (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Column chromatographic separation is mainly used for the isolation of embelin (Chitra et al ., 2003a). Application of aromatic hydrotropes for the isolation of embelin was demonstrated by Latha (2006). Reports on the analytical procedures of embelin are rather limited.…”

Section: Introductionmentioning

confidence: 99%

RP‐HPLC‐DAD method for the estimation of embelin as marker in Embelia ribes and its polyherbal formulations

Madhavan¹,

Arimboor²,

Arumughan³

2011

Biomedical Chromatography

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Evidence-based herbal products with assured quality are assuming importance for complementary and alternative medicine. Traditional medicines by and large are not standardized and validated to meet the new requirements. In the present study, marker (embelin)-based standardization of a major medicinal plant, Embelia ribes and its polyherbal formulations was attempted. Conditions for the quantitative extraction of the marker compound embelin from E. ribes fruits and herbal formulations were also optimized. Reversed-phase high-performance liquid chromatography, coupled with diode array detection (RP-HPLC-DAD) for the quantification of embelin was developed and validated. Satisfactory results were obtained with respect to linearity (15-250 µg/mL), LOD (3.97 µg/mL), LOQ (13.2 µg/mL), recovery (99.4-103.8%) and precision (1.43-2.87%). The applicability of the method was demonstrated with selected phytopharmaceuticals. The present method was sensitive, accurate, simple and reproducible and therefore can be recommended for marker-based standardization, and quality assurance of E. ribes herbal formulations.

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Selective Extraction of Embelin from Embelia ribes by Hydrotropes

Cited by 13 publications

References 20 publications

In-vitro Cytotoxicity Studies on Methanolic Leaf Extract of Embelia Ribes Burm F - an Important Traditional Medicinal Plant of Kerala

In-vitro Cytotoxicity Studies on Methanolic Leaf Extract of Embelia Ribes Burm F - an Important Traditional Medicinal Plant of Kerala

Enhancement of Solubility and Mass-Transfer Coefficient of 1,2-Dihydroxy-9,10-Anthraquinone (Alizarin) Through Hydrotropy

RP‐HPLC‐DAD method for the estimation of embelin as marker in Embelia ribes and its polyherbal formulations

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