Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field–temperature treatment

Postma, P.R.; Pataro, Gianpiero; Capitoli, M.; Barbosa, María J.; Wijffels, René H.; Eppink, M.H.M.; Olivieri, Giuseppe; Ferrari, Giovanna

doi:10.1016/j.biortech.2015.12.012

Cited by 151 publications

(101 citation statements)

References 28 publications

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“…The protein yields obtained with the wild type (cc-124) after PEF treatment are similar to those found for other microalgae with a rigid outer cell wall, like Chlorella vulgaris , Neochloris oleoabundans , and Auxenochlorella protothecoides . 6−8 The study of ‘t Lam et al 7 even showed that PEF allowed at least a 4-fold lower protein release than bead milling. It is therefore reasonable that in general, protein release by means of PEF is limited by the presence of an outer cell wall.…”

Section: Resultsmentioning

confidence: 99%

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Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field

Lam

Kolk

Chordia

et al. 2017

ACS Sustainable Chem. Eng.

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Pulsed electric field (PEF) is considered to be a very promising technology for mild cell disruption. The application of PEF for microalgae that have a rigid cell wall, however, is hampered by the presence of that rigid outer cell wall. A cell wall free mutant of C. reinhardtii was used to mimic pretreated microalgae with removed cell wall, to investigate the possibility of using PEF for protein release from microalgae. A complete release of hydrophilic proteins from the cell wall free mutants was observed whereas PEF treatment on the cell wall containing species resulted in substantially lower protein yields. Additional experiments showed that even at low energy input (0.05 kWh/kgbiomass), still about 70% of the proteins could be released with respect to bead beating as reference. These released proteins were water-soluble while the hydrophobic chlorophyll remained mainly entrapped in cell particles. SEM-analysis of these cell particles showed that PEF only opened the cells, instead of completely fragmenting them into smaller particles. These results indicate that PEF is an energy-efficient cell disruption method for selective release of water-soluble proteins, after the microalgal outer cell wall is removed. Enzymatic pretreatment to degrade the cell walls before PEF treatment was shown to be an efficient method to remove the cell wall.

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Section: Resultsmentioning

confidence: 99%

“…13 This would explain why only small components such as ions were successfully released in previous research while most proteins remained entrapped after PEF treatment. 6−8 …”

Section: Introductionmentioning

confidence: 99%

Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field

Lam

Kolk

Chordia

et al. 2017

ACS Sustainable Chem. Eng.

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“…Since then, PEF has been demonstrated to increase the yield of several high-value microalgae components, including lipids, carbohydrates, carotenoids and chlorophyll [94,95,96,97,98]. Protein yield from Chlorella sp.…”

Section: Protein Extraction Methodsmentioning

confidence: 99%

Algal Proteins: Extraction, Application, and Challenges Concerning Production

Bleakley

Hayes

2017

Foods

728

418

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Population growth combined with increasingly limited resources of arable land and fresh water has resulted in a need for alternative protein sources. Macroalgae (seaweed) and microalgae are examples of under-exploited “crops”. Algae do not compete with traditional food crops for space and resources. This review details the characteristics of commonly consumed algae, as well as their potential for use as a protein source based on their protein quality, amino acid composition, and digestibility. Protein extraction methods applied to algae to date, including enzymatic hydrolysis, physical processes, and chemical extraction and novel methods such as ultrasound-assisted extraction, pulsed electric field, and microwave-assisted extraction are discussed. Moreover, existing protein enrichment methods used in the dairy industry and the potential of these methods to generate high value ingredients from algae, such as bioactive peptides and functional ingredients are discussed. Applications of algae in human nutrition, animal feed, and aquaculture are examined.

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“…The results obtained in the present work are in agreement with other studies in which PEF was evaluated for extraction (Luengo et al ., ; Postma et al ., ). Postma et al .…”

Section: Resultsmentioning

confidence: 99%

The effect of temperature and moderate electric field pre‐treatment on carotenoid extraction from Heterochlorella luteoviridis

Jaeschke

Merlo

Mercali

et al. 2018

Int J of Food Sci Tech

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Summary Moderate electric field (MEF) was evaluated as a pre‐treatment (0–180 V) to carotenoid extraction at four different temperatures (30, 40, 50 and 60 °C) followed by a extraction step using ethanol/water as solvent (75% of ethanol, v/v). During the extraction step, samples were collected at 1, 5, 30, 40 and 50 min. Results showed that MEF allowed higher carotenoid concentration at the beginning of the extraction step at 30, 40 and 50 °C, leading to better extraction results along the extraction time. A different behaviour was observed at 60 °C probably due to the thermal effects on the cell and to carotenoids thermal degradation. The highest extraction yield, 86% of the total carotenoid content (compared to exhaustive extraction), was achieved at both 40 and 50 °C with the MEF pre‐treatment, indicating that a high extraction yield can be obtained even at relatively low temperatures (40 °C).

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Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field–temperature treatment

Cited by 151 publications

References 28 publications

Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field

Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field

Algal Proteins: Extraction, Application, and Challenges Concerning Production

The effect of temperature and moderate electric field pre‐treatment on carotenoid extraction from Heterochlorella luteoviridis

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