Selective induction of antibody effector functional responses using MF59-adjuvanted vaccination

Adjuvanted SARS-CoV-2 spike protein elicits neutralizing antibodies and CD4 T cell responses after a single immunization in mice

“…Adjuvants can direct the T-helper response [ 36 , 57 ] and shape humoral immunity by influencing the magnitude, breadth and affinity of the antibody response [58] in addition to inducing specific antibody subclasses with different Fc-based effector functions [59] . Here we tested three different adjuvant systems together with the pre-fusion stabilized [60] SARS-CoV-2 (S-2P) spike trimer protein [18] .…”

Protein-based, but not viral vector alone, HIV vaccine boosting drives an IgG1-biased polyfunctional humoral immune response

“…For example, whereas VAX003 and RV144 used alum as an adjuvant, the use of MF59 and AS01 as adjuvants has demonstrated induction of superior antibody functionality and increased IgG1 levels (22). In a previous study, using a MF59-adjuvanted influenza vaccine demonstrated the induction of robust IgG1, along with increasing IgG4 titers (23). Surprisingly, although MF59 induced robust phagocytic and complement fixing antibodies in parallel to increasing antibody titers, the vaccine-induced immune response lacked the ability to recruit NK cells, a function that has been linked to reduced risk of infection in the RV144 correlates analyses (11).…”

Distinct Early Serological Signatures Track with SARS-CoV-2 Survival

“…For detection of antibody-dependent NK cell activity, an ELISA-based approach was used, as described ( Boudreau et al., 2020 ). Briefly, plates were coated with 2 μg/mL of antigen (as mentioned above) and samples were added at a 1:50 dilution and incubated for 2h at 37°C.…”