Selective isolation and oligotrophic growth of Candida on nutrient-free silica gel medium

Wainwright, Milton; Altalhi, Abdulla

doi:10.1099/00222615-48-12-1130

Cited by 7 publications

(5 citation statements)

References 3 publications

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“…Isolates from other substrates were obtained following the methods described by Zhang et al. (2017) and further screened with carbon-free silica gel medium to select the oligotrophic strains ( Wainwright & Al-Talhi 1999 ). All the Didymellaceae isolates were primarily identified based on morphology and ITS sequence data, which distinguished them from other groups of fungi.…”

Section: Methodsmentioning

confidence: 99%

Didymellaceae revisited

Chen¹,

Hou²,

Duan³

et al. 2017

Studies in Mycology

210

170

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The Didymellaceae is one of the most species-rich families in the fungal kingdom, and includes species that inhabit a wide range of ecosystems. The taxonomy of Didymellaceae has recently been revised on the basis of multi-locus DNA sequence data. In the present study, we investigated 108 Didymellaceae isolates newly obtained from 40 host plant species in 27 plant families, and various substrates from caves, including air, water and carbonatite, originating from Argentina, Australia, Canada, China, Hungary, Israel, Italy, Japan, South Africa, the Netherlands, the USA and former Yugoslavia. Among these, 68 isolates representing 32 new taxa are recognised based on the multi-locus phylogeny using sequences of LSU, ITS, rpb2 and tub2, and morphological differences. Within the Didymellaceae, five genera appeared to be limited to specific host families, with other genera having broader host ranges. In total 19 genera are recognised in the family, with Heracleicola being reduced to synonymy under Ascochyta. This study has significantly improved our understanding on the distribution and biodiversity of Didymellaceae, although the placement of several genera still need to be clarified.

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Section: Methodsmentioning

confidence: 99%

Didymellaceae revisited

Chen¹,

Hou²,

Duan³

et al. 2017

Studies in Mycology

210

170

View full text Add to dashboard Cite

show abstract

“…It was shown that F. oxysporum was able to oxidize 13 % of thiosulfate, which was added when grown in oligotrophic conditions. It was sufficient, according to the authors, for 25 % of energy biomass accumulation by this fungus [10,34,35].…”

Section: Analysis Of Modern Data On Ecological Physiological and Biomentioning

confidence: 87%

Adaptation of Microfungi to Chronic Ionizing Radiation. New Facts and Hypotheses

Tugay¹,

Tugay²

2017

Mikrobiol. Z.

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“…Although numerous fungi have been reported from various oligotrophic habitats, most are likely facultative rather than obligate oligotrophic fungi (Parkinson et al 1989(Parkinson et al , 1990Wainwright 1993;Wainwright and Al-Talhi 1999;Connell and Staudigel 2013;Liu et al 2013;Godinho et al 2015;Jiang et al 2017). Hitherto only 18 species have been proven for being able to grow on SGM (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Since agar contains available carbon, silica gel is more preferable for the isolation of oligocarbotrophic fungi for laboratory studies (Payton et al 1976;Wainwright and Grayston 1988). Up to now, a wide range of fungi have been isolated from oligotrophic habitats, while only a few of them were confirmed possessing the ability for growth on carbon free Silica Gel Medium (SGM) (Mirocha and DeVay 1971;Wainwright and Grayston 1988;Parkinson et al 1989Parkinson et al , 1990Wainwright 1993;Wainwright and Al-Talhi 1999;Connell and Staudigel 2013;Liu et al 2013;Godinho et al 2015) (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“…7H 2 O 0.01 g in 1 L ultrapure water (UPW); (b) orthophosphoric acid, 20 mL in 100 mL of UPW; (c) silicic acid 10 g and KOH 7 g added to 100 mL of UPW. Gels were prepared by mixing 10 mL of solution (a) with 10 mL of solution (c) and 2 mL of solution (b) into an autoclaved plastic Petri dish (9 cm) (Wainwright and Al-Talhi 1999). All glassware used in the preparation of silica gel medium were washed with chromic acid and then rinsed with ultrapure water (Parkinson et al 1989).…”

Section: Introductionmentioning

confidence: 99%

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Oligotrophic fungi from a carbonate cave, with three new species ofCephalotrichum

2017

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Oligotrophs are microorganisms that can grow in environments where concentrations of nutrients are low or even absent. Caves are typical oligotrophic environments distinctly characterised by constant low temperature, high humidity, scarcity of organic matter and darkness, which encompass a high diversity of fungi. In our investigation of microorganisms from carbonate caves in China, 169 strains belonging to at least 84 taxa were isolated using oligotrophic carbon free silica gel medium (SGM). Cephalotrichum appeared to be one of the dominant genera. Further morphological comparisons and molecular phylogenetic analyses using DNA sequences of four loci (LSU, ITS, TUB2 and EF-1α) revealed that the 30 strains of Cephalotrichum represent three new species, which are described and named C. guizhouense, C. laeve and C. oligotriphicum. This study also significantly improved our understanding on fungi being able to grow on carbon free medium, with the known species increased from 18 to 99.

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Selective isolation and oligotrophic growth of Candida on nutrient-free silica gel medium

Cited by 7 publications

References 3 publications

Didymellaceae revisited

Didymellaceae revisited

Adaptation of Microfungi to Chronic Ionizing Radiation. New Facts and Hypotheses

Oligotrophic fungi from a carbonate cave, with three new species ofCephalotrichum

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