Selective laser-melted fully biodegradable scaffold composed of poly(<scp>d</scp>,<scp>l</scp>-lactide) and β-tricalcium phosphate with potential as a biodegradable implant for complex maxillofacial reconstruction:<i>In vitro</i>and<i>in vivo</i>results

Smeets, Ralf; Barbeck, Mike; Hanken, Henning; Fischer, Horst; Lindner, Markus; Heiland, Max; Wöltje, Michael; Ghanaati, Shahram; Kolk, Andreas

doi:10.1002/jbm.b.33660

Cited by 15 publications

(16 citation statements)

References 44 publications

(88 reference statements)

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“…The 36 included animal studies were published in scientific journals in English, during the period 2007-2017 [ [35], [36], [37], [38], [39], [40], [41], [42], [43], [44], [45], [46], [47], [48], [49], [50], [51], [52], [53], [54], [55], [56 ], [57], [58], [59], [60], [61], [62], [63], [64], [65], [66], [67], [68], [69], [70], [71]]. One study was published as poster presentation [72].…”

Section: Animal Studies 51 Study Selectionmentioning

confidence: 99%

“…The vast majority of the defects were calvarial [35,37,[39], [40], [41], [42], [44], [45], [46], [47], [48], [49], [50], [52], [53], [54], [55], [56], [57], [58], [59], [60], [61 ], [62], [64], [65], [66], [67] [70], [71], [72]]. In approximately half of the studies, the scaffold was enhanced with biomolecules, such as BMP-2 and/or stem cells [35,36,39,40,42,[44], [45], [46], [47], [51], [52], [53], [54],57,58, [65], [66], [67], [68]].…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Mostly the higher porosity facilitates more rapid biodegradation [49]. This issue becomes even more difficult, since the fact that degradation kinetics and mechanical properties are irreversibly proportional (faster degradation, lower mechanical strength) [44,57]. The high rate ensures fast bone turnover, avoids any inflammatory process and protects from fibro gingival dehiscence and invasion [57].…”

Section: Scaffold Kinetics and Biocompatibilitymentioning

confidence: 99%

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Applications of 3D printing on craniofacial bone repair: A systematic review

Maroulakos

Kamperos

Tayebi

et al. 2019

Journal of Dentistry

115

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Objectives Three-dimensional (3D) bioprinting, a method derived from additive manufacturing technology, is a recent and ongoing trend for the construction of 3D volumetric structures. The purpose of this systematic review is to summarize evidence from existing human and animal studies assessing the application of 3D printing on bone repair and regeneration in the craniofacial region. Data & sources A rigorous search of all relevant clinical trials and case series was performed, based on specific inclusion and exclusion criteria. The search was conducted in all available electronic databases and sources, supplemented by a manual search, in December 2017. Study selection 43 articles (6 human and 37 animal studies) fulfilled the criteria. The human studies included totally 81 patients with craniofacial bone defects. Titanium or hydroxylapatite scaffolds were most commonly implanted. The follow-up period ranged between 6 and 24 months. Bone repair was reported successful in nearly every case, with minimal complications. Also, animal intervention studies used biomaterials and cells in various combination, offering insights into the techniques, through histological, biochemical, histomorphometric and microcomputed tomographic findings. The results in both humans and animals, though promising, are yet to be verified for clinical impact. Conclusions Future research should be focused on well-designed clinical trials to confirm the short-and long-term efficacy of 3D printing strategies for craniofacial bone repair. Clinical significance Emerging 3D printing technology opens a new era for tissue engineering. Humans and animals on application of 3D printing for craniofacial bone repair showed promising results which will lead clinicians to investigate more thoroughly alternative therapeutic methods for craniofacial bone defects.

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Section: Animal Studies 51 Study Selectionmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Scaffold Kinetics and Biocompatibilitymentioning

confidence: 99%

See 1 more Smart Citation

Applications of 3D printing on craniofacial bone repair: A systematic review

Maroulakos

Kamperos

Tayebi

et al. 2019

Journal of Dentistry

115

View full text Add to dashboard Cite

show abstract

“…COPROGs protect the inserted DNA particles and thus allow more sustainable release avoiding genetic insertion as occurs during viral transfection. Thus, pBMP‐2 seems highly suitable for the osseointegration of implants and bone regeneration, for example, for the functionalizing of scaffolds, if new physiologically structured NB is required. However, in settings in which large‐volume bone defects are apparent, the slow bone formation by pBMP‐2 might be a drawback and rhBMP‐2 could be an option.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of bone regeneration behavior using recombinant human BMP‐2 versus plasmid DNA of BMP‐2

Kolk

Boskov

Haidari

et al. 2018

J Biomedical Materials Res

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Bone regeneration and the osteoinductive capacity of implants are challenging issues in clinical medicine. Currently, recombinant growth factors and nonviral gene transfer are the most frequently investigated methods for bone growth enhancement, although the more favorable method remains unclear. There is a lack of knowledge in literature about the in vivo comparison of these methods for bone regeneration. BMP-2, which is the most commonly used growth factor for osteogenesis, was applied at its most efficient dose as a recombinant growth factor (rhBMP-2) and as a growth-factorencoding copolymer protected gene vector (pBMP-2) in a critical size bone defect (CSD) model to determine the most suitable method for bone regeneration. CSDs were induced bilaterally in 32 Sprague-Dawley rats. RhBMP-2 (62.5 μg) or pBMP-2 (2.5 μg) was embedded in poly(D,L-)lactide-coated titanium discs. Survival times were set at 14, 28, 56, and 112 days. After euthanasia, samples were analyzed via micro-computed tomography, polychrome sequential fluorescent labeling, and immunohistochemistry. Whereas defects in both groups were bridged with new bone after 56 days, rhBMP-2 initially induced ectopic new bone formation that was later remodeled in an unorganized hypodense manner. In contrast, pBMP-2 led to slower but steady bone regeneration with physiological tissue morphology, as confirmed by high osteoblast activity shown by osteocalcin staining. CD68 and TRAP staining verified high osteoclast activity for the rhBMP-2 group. pBMP-2 successfully induced locally controlled physiological bone regeneration, whereas rhBMP-2 triggered rapid and ectopic but insufficient bone formation. Thus, nonviral gene transfer appears to be more favorable for clinical applications.

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“…Bone defects following trauma or ablative surgery result in high demand for new bone (NB) to replace and restore the original shape and function of the lost osseous regions. 1 Although the body possesses its own capacity to heal spontaneously and to integrate metallic implants, osseous regrowth or the integration of implants is not always satisfactory, especially in the elderly population. However, in severe pathological situations, autogenous bone grafting remains one of the most common surgical procedures for skeletal healing.…”

Section: Introductionmentioning

confidence: 99%

A novel nonviral gene delivery tool of BMP‐2 for the reconstitution of critical‐size bone defects in rats

Kolk

Tischer

Koch

et al. 2016

J Biomedical Materials Res

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The osseointegration of bone implants, implant failure, and the bridging of critical-size bone defects are frequent clinical challenges. Deficiencies in endogenous bone healing can be resolved through the local administration of suitable recombinant growth factors (GFs). In preclinical models, gene-therapy-supported bone healing has proven promising for overcoming certain limitations of GFs. We report the dose-dependent bridging of critical-size mandibular bone defects (CSDs) in a rat model using a non-viral BMP-2-encoding copolymer-protected gene vector (pBMP-2) embedded in poly(d, l-lactide) (PDLLA) coatings on titanium discs that were used to cover drill holes in the mandibles of 53 male Sprague Dawley rats. After sacrificing, the mandibles were subjected to micro-computed tomography (µCT), micro-radiography, histology, and fluorescence analyses to evaluate bone regeneration. pBMP-2 in PDLLA-coated titanium implants promoted partial bridging of bone defects within 14 days and complete defect healing within 112 days when the DNA dose per implant did not exceed 2.5 µg. No bridging was observed in untreated control CSDs. Thus, the delivery of plasmid DNA coding for BMP-2 appears to be a potent method for controlled new-bone formation with an inverse dose dependency. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2441-2455, 2016.

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Selective laser-melted fully biodegradable scaffold composed of poly(d,l-lactide) and β-tricalcium phosphate with potential as a biodegradable implant for complex maxillofacial reconstruction:In vitroandin vivoresults

Cited by 15 publications

References 44 publications

Applications of 3D printing on craniofacial bone repair: A systematic review

Applications of 3D printing on craniofacial bone repair: A systematic review

Comparative analysis of bone regeneration behavior using recombinant human BMP‐2 versus plasmid DNA of BMP‐2

A novel nonviral gene delivery tool of BMP‐2 for the reconstitution of critical‐size bone defects in rats

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