Selective Liquid Chromatographic Method for Determination of Fluoxetine in Plasma

Zarghi, Afshin; Kebriaeezadeh, Abbas; Ahmadkhaniha, Reza; Akhgari, Maryam; Rastkari, N.

doi:10.1093/jaoac/84.6.1735

Cited by 16 publications

(7 citation statements)

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“…The activation energies increase with solvent polarity and are higher for this latter radical, with a value of 12.40 kcal mol −1 in water to be compared with 5.38 kcal mol −1 computed for the alkoxyl radical in the same conditions. and gas chromatography are some of the tools included in the methods currently available in the literature [5,[57][58][59]. The choice of applying one or more of such analytical techniques should also take into consideration the stereochemical aspects [6].…”

Section: Fluoxetine Metabolitesmentioning

confidence: 99%

Major Depressive Disorder and Oxidative Stress: In Silico Investigation of Fluoxetine Activity against ROS

et al. 2019

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Major depressive disorder is a psychiatric disease having approximately a 20% lifetime prevalence in adults in the United States (U.S.), as reported by Hasin et al. in JAMA Psichiatry 2018 75, 336–346. Symptoms include low mood, anhedonia, decreased energy, alteration in appetite and weight, irritability, sleep disturbances, and cognitive deficits. Comorbidity is frequent, and patients show decreased social functioning and a high mortality rate. Environmental and genetic factors favor the development of depression, but the mechanisms by which stress negatively impacts on the brain are still not fully understood. Several recent works, mainly published during the last five years, aim at investigating the correlation between treatment with fluoxetine, a non-tricyclic antidepressant drug, and the amelioration of oxidative stress. In this work, the antioxidant activity of fluoxetine was investigated using a computational protocol based on the density functional theory approach. Particularly, the scavenging of five radicals (HO•, HOO•, CH3OO•, CH2=CHOO•, and CH3O•) was considered, focusing on hydrogen atom transfer (HAT) and radical adduct formation (RAF) mechanisms. Thermodynamic as well as kinetic aspects are discussed, and, for completeness, two metabolites of fluoxetine and serotonin, whose extracellular concentration is enhanced by fluoxetine, are included in our analysis. Indeed, fluoxetine may act as a radical scavenger, and exhibits selectivity for HO• and CH3O•, but is inefficient toward peroxyl radicals. In contrast, the radical scavenging efficiency of serotonin, which has been demonstrated in vitro, is significant, and this supports the idea of an indirect antioxidant efficiency of fluoxetine.

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Section: Fluoxetine Metabolitesmentioning

confidence: 99%

Major Depressive Disorder and Oxidative Stress: In Silico Investigation of Fluoxetine Activity against ROS

et al. 2019

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“…In this study, there was no residual effect that could be harmful to humans. In 2001, Zarghi et al developed a method for determination of doxycycline in human plasma using HPLC assay based on a mbondapak C18 column and a lambda (347 nm) UV detector [9]. Reagents like actonitrile, potassium dihydrogen phosphate, 24% perchloric acid aqueous solution were utilized there.…”

Section: Discussionmentioning

confidence: 99%

“…Various methods for quantification of doxycycline in vitro and in vivo have been reported. These include in vitro experimental model [6], fluorimetry [7], TLC-fluorescence scanning densitometry [8] and HPLC [9][10][11][12][13][14][15][16][17][18][19] for the quantification of doxycycline in biological materials. HPLC was also applied for the determination of doxycycline in pharmaceutical formulations [20].…”

Section: Introductionmentioning

confidence: 99%

Quantification of Doxycycline Hyclate in Different Pharmaceutical Samples by UV Assay

Hasan

Haque

Kobir

et al. 2016

IJDDT

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Objective: Doxycycline hyclate is a broad spectrum antibiotic with activity against a wide range of gram-positive and gram-negative bacteria and it is widely used as a pharmacological agent. A simple, selective, linear, precise and accurate ultraviolet detection (UV) method has been developed and applied for the determination of doxycycline hyclate in different pharmaceutical samples. Methods: Acid-base analysis and titrimetric method were utilized to determine the value of pH and moisture content of purchased pharmaceutical samples. A mixture of methanol and hydrochloric acid (0.01N Methanolic HCl) was used to determine the biochemical properties of doxycycline hyclate. UV detector set at 349 nm was used to monitor the effluent. The purified water was used as solvent. Results: In 1% aqueous solution of doxycycline, three samples (4th, 5th and 7th) showed lower pH values of 1.97, 1.98, and 1.99 respectively. Furthermore, the same samples indicated the additional moisture contents of 2.81%, 2.85% and 2.83% respectively while considering the acceptance level (1.4% to 2.8%). The method proved to be linear (R2=0.993), precise (RSD=0.79% for inter-day precision), accurate (Recovery=100.59%) and selective regarding possible impurities and excipients of the samples. The doxycycline content obtained in the sample analysis was within the range of 84.05% to 85.80%. Conclusion: The optimized and validated method may be successfully employed to perform routine quality control analyses. Investigation of the pH, moisture content and potency of doxycycline hyclate in different samples give a general view of local pharmacies trade and ensure that the method applied here was validated for this kind of analysis.

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“…Due to its antiinflammatory properties, it is prescribed for pets in inflamematory issues of the bowel [4,5]. Various methods have been reported for the quantitative determination of DOX both in pharmaceutical preparations and biological samples based on UV-Visible spectrophotometry [6][7][8][9][10], spectrofluorimetry [11][12][13], chromatographic methods [14][15][16][17][18][19][20][21][22] and electrochemical methods [23,24]. Several analytical procedures have been reported for the quantitative determination of TYT including spectrophotometric method [25] and chromatographic methods [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Optimization and validation of a new chromatographic method for the assay of veterinary formulation

et al. 2019

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New, validated and accurate reversed phase HPLC method with UV detection has been established for simultaneous determination of a veterinary binary mixture of doxycycline hydrochloride (DOX) and tylosin tartrate (TYT). The stationary phase was ACE- 126-2546 AQ C-18 (250 × 4.6 mm i.d., 5 μm particle size) column at 25 °C, in an isocratic mode, using mobile phase containing a mixture of methanol: acetonitrile: distilled water in the ratio of 60:20:20 (v:v:v), with 0.01% trichloroacetic acid at the flow rate of 0.8 mL/min and UV detection was performed at 270 nm. The retention times were 4.02±0.01 and 5.62±0.01 mins for DOX and TYT, respectively. Selective determination of the cited veterinary drugs has been developed in their formulation. The method was found to be linear over 1-50 µg/mL for DOX and TYT with mean percentage recoveries 99.62±1.220 and 100.09±1.104%. The method was proven to be accurate, precise and specific. The obtained results were statistically compared with those of the official and reported methods; using Student’s t test, F test and one-way ANOVA, showing no significant difference with high accuracy. Specificity of the applied method was assessed by analysing the laboratory-prepared mixtures and their combined dosage form. The developed method was confirmed according to ICH guidelines. The validated method can be considered as alternative and basic method for the routine determination of this fixed dose combination with minimum sample preparation.

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Selective Liquid Chromatographic Method for Determination of Fluoxetine in Plasma

Cited by 16 publications

References 0 publications

Major Depressive Disorder and Oxidative Stress: In Silico Investigation of Fluoxetine Activity against ROS

Major Depressive Disorder and Oxidative Stress: In Silico Investigation of Fluoxetine Activity against ROS

Quantification of Doxycycline Hyclate in Different Pharmaceutical Samples by UV Assay

Optimization and validation of a new chromatographic method for the assay of veterinary formulation

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