Selective microbial electrosynthesis of methane by a pure culture of a marine lithoautotrophic archaeon

Beese-Vasbender, Pascal Fabien; Grote, Jan-Philipp; Garrelfs, Julia; Stratmann, M.; Mayrhofer, Karl Johann Jakob

doi:10.1016/j.bioelechem.2014.11.004

Cited by 161 publications

(96 citation statements)

References 40 publications

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“…Enhancing electrosynthesis using co-cultures JS Deutzmann and AM Spormann − 600 mV, respectively (Beese-Vasbender et al, 2015a). Interestingly, these rates are roughly in the same order of magnitude as the single cell anodic reduction reaction in Shewanella oneidensis (10 5 -10 6 e − s − 1 cell − 1 ) (Gross and El-Naggar, 2015).…”

Section: Discussionmentioning

confidence: 91%

“…Enhancing electrosynthesis using co-cultures JS Deutzmann and AM Spormann molecular hydrogen was likely a side product during electromethanogenesis by strain IM1, which became obvious at more negative potentials (Beese-Vasbender et al, 2015a). The rates obtained here with the co-culture are about 20 times higher compared with electromethanogenesis using axenic M. maripaludis at − 600 mV (Lohner et al, 2014) (0.6-1.2 μmol h − 1 cm − 2 compared with 0.05 μmol h − 1 cm − 2 ).…”

Section: Efficient Interspecies Hydrogen Transfer In Mixed Electrosynmentioning

confidence: 99%

“…The genus Methanobacterium became enriched in several studies investigating electromethanogenesis (Pozo et al, 2015;Siegert et al, 2015) and it has been shown in pure culture that Methanobacterium strain IM1 not only catalyzed electrosynthetic methanogenesis but also accumulated hydrogen during electromethanogenesis (Beese-Vasbender et al, 2015a), which can create a niche for other microorganisms. In homoacetogenic communities the role of distinct electron uptake strains seems to be more elusive.…”

Section: Efficient Interspecies Hydrogen Transfer In Mixed Electrosynmentioning

confidence: 99%

“…Electrosynthetic methane formation has been achieved using mixed cultures (Cheng et al, 2009, Siegert et al, 2015 or pure strains such as Methanococcus maripaludis and Methanobacterium sp. (Lohner et al, 2014;Beese-Vasbender et al, 2015a). Multi-carbon compounds such as acetate have been synthesized on biocathodes using a diversity of homoacetogenic strains (Nevin et al, 2010(Nevin et al, , 2011Deutzmann et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced microbial electrosynthesis by using defined co-cultures

2016

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Microbial uptake of free cathodic electrons presents a poorly understood aspect of microbial physiology. Uptake of cathodic electrons is particularly important in microbial electrosynthesis of sustainable fuel and chemical precursors using only CO 2 and electricity as carbon, electron and energy source. Typically, large overpotentials (200 to 400 mV) were reported to be required for cathodic electron uptake during electrosynthesis of, for example, methane and acetate, or low electrosynthesis rates were observed. To address these limitations and to explore conceptual alternatives, we studied defined co-cultures metabolizing cathodic electrons. The Fe(0)-corroding strain IS4 was used to catalyze the electron uptake reaction from the cathode forming molecular hydrogen as intermediate, and Methanococcus maripaludis and Acetobacterium woodii were used as model microorganisms for hydrogenotrophic synthesis of methane and acetate, respectively. The IS4-M. maripaludis co-cultures achieved electromethanogenesis rates of 0.1-0.14 μmol cm − 2 h − 1 at − 400 mV vs standard hydrogen electrode and 0.6-0.9 μmol cm − 2 h − 1 at − 500 mV. Co-cultures of strain IS4 and A. woodii formed acetate at rates of 0.21-0.23 μmol cm − 2 h − 1 at − 400 mV and 0.57-0.74 μmol cm − 2 h − 1 at − 500 mV. These data show that defined co-cultures coupling cathodic electron uptake with synthesis reactions via interspecies hydrogen transfer may lay the foundation for an engineering strategy for microbial electrosynthesis.

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Section: Discussionmentioning

confidence: 91%

Section: Efficient Interspecies Hydrogen Transfer In Mixed Electrosynmentioning

confidence: 99%

Section: Efficient Interspecies Hydrogen Transfer In Mixed Electrosynmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced microbial electrosynthesis by using defined co-cultures

2016

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“…As reported a decade later by Beese-Vasbender et al 2015a, the electron uptake from electrodes by D. corrodens is not limited to iron serving as the sole electron donor, but can also take place on graphite and doped germanium cathodes.…”

Section: Elucidation Of Autotrophic Sulfate-reducing Mechanisms In Bimentioning

confidence: 92%

Bio-Electrochemical process for Metal and Sulfur Recovery from Acid Mine Drainage

Zamora¹,

Alonso²

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Water recycling and resource recovery from acid mine drainage (AMD) are increasingly being regarded as desirable practices with direct benefits for the environment and the operational and economic viability of the resources sector.This thesis proves the concept of a novel bioelectrochemical system (BES) for the direct electrode-driven resource recovery and practically permanent AMD treatment. The technology consists of a two-cell bioelectrochemical setup to enable the removal of sulfate from the ongoing reduction-oxidation sulfur cycle, thereby also reducing salinity, without external addition of chemicals.In particular, the goals of this thesis are: (i) to enrich a sulfate reducing bacterial community capable of directly utilising a carbon based cathode as electron donor for autotrophic sulfate reduction, or via bioelectrochemically-produced H 2 ; (ii) to elucidate the electron flux pathways of autotrophic sulfate reduction and microbial interactions in cathodic mixed cultures; (iii) to develop a high-rate sulfate reducing bioelectrochemical reactor based on high surface area electrode materials; (iv) to design and implement a combined chemical-free bioelectrochemical process that enables the sulfur, metal and water recovery from AMD.In order to elucidate whether cathodes can effectively release electrons and act as the only electron donor to support sulfate reduction process, the effect of cathode potential and inoculum source were evaluated using electrochemical tools, including the recording of chronoamperometry and cyclic/linear sweep voltammetry (Chapter 5). Electrochemical and off-gas analysis coupled to liquid phase sampling was carried out to determine the electron fluxes from the electrode to the final electron acceptor (sulfate) during autotrophic sulfate reduction (Chapter 6). Fluorescence in situ hybridization (FISH) and digital image analysis (DAIME) of the microbial communities in z-stack confirmed the microbial stratification. After obtaining a well-functioning biocathode for autotrophic sulfate reduction, its performance was experimentally optimised in terms of high-surface area electrode materials, like multi-wall carbon nanotubes on reticulated vitreous carbon (MWCNT-RVC) and carbon granules (CG) (Chapter 7). Finally, a novel BES was tested for AMD treatment , outcompeting methanogens and homoacetogens for the hydrogen without the need to add chemical inhibitors.The findings of this thesis show that inexpensive CG can achieve higher current-tosulfide efficiencies at lower power consumption than the nano-modified three-dimensional MWCNT-RVC. Sequencing analysis of the 16S rRNA gene on day 58 revealed that MWCNT-RVC retained the bacteria and archaea population from the inoculum, while CG electrode surface was able to select for bacteria over archaea.The feasibility to integrate a two-stage BES was proven with a lab-scale setup for AMD treatment. Using AMD, the BES operation enhanced the sulfate reduction rate ( The solid metal-sludge was 2 times less voluminous and 9 times more readily s...

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