Selective modulation of monocyte and neutrophil responses with activated protein C in preterm infants

Eliwan, Hassan; Watson, R. William G.; Melo, Ashanty M; Kelly, Lynne; Omer, Murwan; Jafar, Ali; O’Hare, F; Downey, Paul; Mooney, Eoghan E.; O’Neill, Amanda; Blanco, Alfonso; Regan, Irene; Philbin, Brian; O'Rourke, Michelle; Nolan, Beatrice; Smith, Owen; Molloy, Eleanor J.

doi:10.1080/14767058.2023.2183467

The Journal of Maternal-Fetal & Neonatal Medicine

2023

DOI: 10.1080/14767058.2023.2183467

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Selective modulation of monocyte and neutrophil responses with activated protein C in preterm infants

Hassan Eliwan

R. William G. Watson

Ashanty M Melo

et al.

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2024

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Impact of Extreme Prematurity, Chorioamnionitis, and Sepsis on Neonatal Monocyte Characteristics and Functions

Qazi,

Govindaraj,

Martí

et al. 2024

J Innate Immun

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Introduction: The innate branch of the immune system is important in early life, in particular for infants born preterm. Methods: We performed a longitudinal analysis of the peripheral monocyte compartment in extremely preterm children from a randomized placebo-controlled study of probiotic supplementation. PBMCs and fecal samples were collected at several timepoints during the first months of life. Monocyte characteristics were analyzed by flow cytometry and LPS-stimulated PBMC culture supernatants were analyzed by Luminex or ELISA. Plasma cytokines and gut microbiota composition were analyzed by ELISA and 16S rRNA-sequencing, respectively. Results: The extremely preterm infants had persistent alterations in their monocyte characteristics, that were further aggravated in chorioamnionitis cases. They showed a markedly reduced TLR4 expression and hampered LPS-stimulated cytokine responses 14 days after birth. Notably, at later time-points, TLR4 expression and LPS-responses no longer correlated. Sepsis during the first weeks of life strongly associated with increased proinflammatory, and reduced IL-10, responses also at post menstrual week 36. Further, we report a correlation between gut microbiota features and monocyte phenotype and responses, but also that probiotic supplementation associated with distinct monocyte phenotypic characteristics, without significantly influencing their responsiveness. Conclusion: Extremely preterm infants have monocyte characteristics and functional features that deviate from infants born full-term. Some of these differences persist until they reach an age corresponding to full-term, potentially making them more vulnerable to microbial exposures during the first months of life.

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Impact of Extreme Prematurity, Chorioamnionitis, and Sepsis on Neonatal Monocyte Characteristics and Functions

Qazi,

Govindaraj,

Martí

et al. 2024

J Innate Immun

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show abstract

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Selective modulation of monocyte and neutrophil responses with activated protein C in preterm infants

Cited by 1 publication

References 32 publications

Impact of Extreme Prematurity, Chorioamnionitis, and Sepsis on Neonatal Monocyte Characteristics and Functions

Impact of Extreme Prematurity, Chorioamnionitis, and Sepsis on Neonatal Monocyte Characteristics and Functions

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