Selective Response to Bacterial Infection by Regulating Siglec-E Expression

Wu, Yin; Yang, Darong; Liu, Runhua; Wang, Lizhong; Chen, Guo‐Yun

doi:10.1016/j.isci.2020.101473

Cited by 7 publications

(11 citation statements)

References 68 publications

(131 reference statements)

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“…Cells overexpressing Siglec-E with mutated Tyr432 also produced lower amounts of ROS following E.coli infection. This confirmed that Tyr432 in the ITIM domain of Siglec-E is needed for its association with NOX2 subunit p47 phox which promotes ROS production (41). Interestingly, treatment of primary human neutrophils with a synthetic Siglec-9 agonist (pS9L) or anti-Siglec-9 antibody also leads to ROS generation which is inhibited upon SHP-1/2 inhibitor treatment.…”

Section: Siglec-based Modulation Of Ros Productionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

“…Siglec-E knockout neutrophils infected with non-sialylated Escherichia coli strains (Gram-negative bacteria) produced significantly less amount of ROS than wild-type neutrophils, suggesting that Siglec-E promoted ROS generation ( 41 ). Silencing Siglec-E or Siglec-9 (the human equivalent of Siglec-E) also reduced ROS generation in neutrophils/THP-1 cells after E.coli infection ( 41 ). Immunoprecipitation revealed that endogenous Siglec-E associates with NOX2 subunits (gp91 phox /p47 phox ) in E.coli -infected murine neutrophil and Tyr432 residue was found to be critical for Siglec-E-p47 phox binding.…”

Section: Introductionmentioning

confidence: 99%

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Siglecs Modulate Activities of Immune Cells Through Positive and Negative Regulation of ROS Generation

2021

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Reactive oxygen species (ROS) are a group of oxygen-containing highly-reactive molecules produced from oxidative metabolic processes or in response to intracellular signals like cytokines and external stimuli like pathogen attack. They regulate a range of physiological processes and are involved in innate immune responses against infectious agents. Deregulation of ROS contributes to a plethora of disease conditions. Sialic acids are carbohydrates, present on cell surfaces or soluble proteins. Sialic acid-binding immunoglobulin-like lectins (Siglecs) recognize and bind to sialic acids. These are widely expressed on various types of immune cells. Siglecs modulate immune activation and can promote or inhibit ROS generation under different contexts. Siglecs promote ROS-dependent cell death in neutrophils and eosinophils while limiting oxidative stress associated with chronic obstructive pulmonary disease (COPD), sickle cell disease (SCD), coronavirus disease-2019 (COVID-19), etc. This review distinguishes itself in summarizing the current understanding of the role of Siglecs in moderating ROS production and their distinct effect on different immune cells; that ultimately determine the cellular response and the disease outcome. This is an important field of investigation having scope for both expansion and medical importance.

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Section: Siglec-based Modulation Of Ros Productionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Siglecs Modulate Activities of Immune Cells Through Positive and Negative Regulation of ROS Generation

2021

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“…The CD24/Siglec-10 signaling pathway protects cancer cells from the immune system, indicating a potential target for cancer immunotherapy (8). Another Siglec, Siglec-E, negatively regulates the inflammatory response in bacterial infection (9,10).…”

Section: Introductionmentioning

confidence: 99%

The role of the Siglec-G ITIM domain during bacterial infection

Yang

Chen

2022

Cell Mol Biol (Noisy-le-grand)

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Siglecs, membrane-bound lectins of the sialic acid-binding immunoglobulin superfamily, inhibit immune responses by recruiting tyrosine phosphatases (e.g., SHP-1 and SHP-2) through their cytoplasmic immunoreceptor tyrosine-based inhibition motif (ITIM) domain. The role of Siglecs in infection has been extensively studied, but downstream signaling through the ITIM domain remains unclear. Here, we used a GST pull-down assay to identify additional proteins associated with the ITIM domain during bacterial infection. Gdi2 bound to ITIM under normal homeostasis, but Rab1a was recruited to ITIM during bacterial infection. Western blot analysis confirmed the presence of SHP-1 and SHP-2 in eluted ITIM-associated proteins under normal homeostasis. We confirmed the association of ITIM with Gdi2 or Rab1a by transfection of corresponding expression vectors in 293T cells followed by immunoprecipitation-western blot assay. Thus, ITIM’s role in the inhibition of the immune response during bacterial infection may be regulated by interaction with Gdi2 and Rab1a in addition to SHP-1 and SHP-2.

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Salen-manganese complex-based nanozyme with enhanced superoxide- and catalase-like activity for wound disinfection and anti-inflammation

Dai

Liu

et al. 2023

Chemical Engineering Journal

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Selective Response to Bacterial Infection by Regulating Siglec-E Expression

Cited by 7 publications

References 68 publications

Siglecs Modulate Activities of Immune Cells Through Positive and Negative Regulation of ROS Generation

Siglecs Modulate Activities of Immune Cells Through Positive and Negative Regulation of ROS Generation

The role of the Siglec-G ITIM domain during bacterial infection

Salen-manganese complex-based nanozyme with enhanced superoxide- and catalase-like activity for wound disinfection and anti-inflammation

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