Selective separation and identification of metabolite groups of Polygonum cuspidatum extract in rat plasma using dispersion solid-phase extraction by magnetic molecularly imprinted polymers coupled with LC/Q-TOF-MS

Abstract

In this work, magnetic molecularly imprinted polymers (MMIPs) were successfully prepared for specific recognition and selective enrichment of metabolite groups of Polygonum cuspidatum extract in rat plasma.

Analysis of the chemical components of Qixianqingming granules and their metabolites in rats by UPLC‐ESI‐Q‐TOF‐MS

“…M4 and M13 all showed m / z 307 [M‐H] − , which produced the main fragment ions at m / z 227 in their MS E spectra, indicating the presence of a sulfate. Therefore, compounds M4 and M13 were characterized as resveratrol‐ O ‐sulfate, according to reference . M7, M8, and M9 were detected [M‐H] − at m / z 485, and fragment ions were detected at m / z 309, 229, and 187, suggesting the addition of two hydrogen atoms compared with those in the structure of M3.…”

“…Fragment ions at m / z 269, 241, and 225 were diagnosed ions of emodin, and the fragment ions at m / z 445 and 269 were formed by successive losses of sulfate (80 Da) and glucuronic acid (176 Da) from the [M‐H] − . Therefore, M11 was interpreted to be emodin‐ O ‐glucuronide sulfate, according to the literature . M16 produced [M‐H] − at m / z 461 and fragment ions at m / z 285, 268, 257, 255, 241, 227, and 211.…”

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

“…But, to achieve a complete elimination of MEs, a selective extraction should be planned and performed [19,20]. Recently, the development of molecular imprinted technology (MIP) should provide the analyst with new opportunities in terms of selective extraction, high recovery percentage and low MEs [21,22], but unfortunately this technology is not yet commercially available. Generally, the more similar the polarity between the target analytes and the matrix composition, the less chance there is for efficient and selective extraction step using the common extraction procedures.…”

Identification and characterization of fluvastatin metabolites in rats by UHPLC/Q‐TOF/MS/MS and in silico toxicological screening of the metabolites

“…So comprehensive study of biotransformation pathways and the knowledge of the possible toxicity of metabolites are prerequisite for the drug discovery and development . Over the years, liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI/MS/MS) in combination with accurate mass measurements has been widely used for the identification and characterization of metabolites …”

“…[10] Over the years, liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS) in combination with accurate mass measurements has been widely used for the identification and characterization of metabolites. [11][12][13][14] Comprehensive literature search revealed that there are several analytical and bioanalytical reports on chromatographic determination of FLU, [15] such as UV determination, [16] kinetic studies on FLU photo degradation, [17] chiral evaluation by HPLC-ESI/MS, [18] determination of FLU and other drugs used in cardiovascular therapy by liquid chromatography-mass spectrometry (LC-MS/MS), [19] fluorometric determination of its enantiomers, [20] effects of clopidogrel [21] and fluconazole [22] on its pharmacokinetics, UV determination of simvastatin and FLU in serum and formulation, [23] HPLC method for determination of FLU and its metabolites in plasma, [24] and quantification of FLU by LC-MS/MS. [25] A few metabolites of FLU have been reported [26] by using HPLC coupled with the radioactive monitoring with a run time of 120 min.…”