Selective visualization of rabbit knee cartilage using MR imaging with a double-contrast agent

Kido, Masamitsu; Ikoma, Kazuya; Hara, Yusuke; Matsuda, Ken‐ichi; Kawata, Mitsuhiro; Umeda, Masahiro; Kubo, Toshikazu

doi:10.1002/jmri.24282

Cited by 2 publications

(7 citation statements)

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“…A RM é reconhecida como um método não invasivo para examinar patologias articulares e uma referência efetiva para avaliar a reparação em cartilagens (Goebel et al 2012, Rodríguez--Merchán 2012, Bekkers et al 2013, Endo et al 2015, Obara et al 2015. Além disso, a RM também pode ser útil na identificação de lesões ósseas ocultas à radiografia convencional, incluindo as contusões ósseas e fraturas osteocondrais, que representam sítios de hiperemia, edema ou hemorragia óssea (Grossi et al 2001, Kido et al 2014.…”

Section: Discussionunclassified

“…Permite a visualização detalhada dos tecidos intra-articulares, fornecendo contraste diferenciado entre os tecidos moles em diferentes ponderações de imagem e com capacidade multiplanar (Muglia et al 2001, Efe et al 2012, Blackman et al 2013, Endo et al 2015. A partir da utilização de técnicas específicas para a região articular, a RM fornece informações sobre as alterações de sinal do tecido cartilaginoso, da morfologia da superfície articular e do osso subcondral (Rodrigues & Camanho 2010, Kido et al 2014. Além disso, em tratamentos com enxertos osteocondrais, proporciona a obtenção de dados estruturais importantes a respeito do preenchimento do defeito, da integração do tecido de reparação com a cartilagem circundante e do aspecto da superfície articular (Nho et al 2008, Battaglia et al 2011.…”

Section: Introductionunclassified

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Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

et al. 2017

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ABSTRACT. - This study aimed to determine the characteristics and applicability of magnetic resonance imaging (MRI) in the evaluation of autogenous osteochondral graft in intact or macerated format, with or without insulin-like growth factor type 1 (IGF-1) used in repair of cartilage lesions induced in rabbits. Nine New Zealand rabbits were used, in which 18 stifle joints underwent grafting procedure in the femoral trochlear groove. These were divided into four groups, referred as intact osteochondral graft + IGF-1 (n=5), intact osteochondral graft + saline solution (n=4), macerated osteochondral graft + IGF-1 (n=5) and macerated osteochondral graft + saline solution (n=4). Animals were euthanized 12 weeks after surgery and the joints were subjected to MRI using a high magnetic field scanner of 1.5 Tesla. In addition, samples of grafting sites were subjected to anatomopathological examination. The MRI was effective as a noninvasive method to evaluate the repair tissue in osteochondral grafts in articular cartilage of the femur of rabbits by providing complementary data to macroscopic and histological examinations. Through these images and anatomopathological examinations satisfactory results were observed in relation to the repair process of autogenous osteochondral grafts in cartilage of rabbits, regardless of its format or the addition of IGF-1. RESUMO.-Este trabalho teve o objetivo de determinar as características e a aplicabilidade do exame de ressonância magnética na avaliação de enxerto ostecondral autógeno, em formato íntegro ou macerado, associado ou não ao fator de crescimento semelhante à insulina tipo 1 (IGF-1), utilizado no reparo de lesões induzidas na cartilagem articular de coelhos. Foram utilizados 9 coelhos da linhagem Nova Zelândia, em que as 18 articulações fêmoro-tíbio-patelares foram submetidas à enxertia osteocondral autógena no sulco troclear femoral. Estas foram divididas em quatro grupos, denominados como enxerto osteocondral íntegro + IGF-1 (n=5), enxerto osteocondral íntegro + solução fisioló-gica (n=4), enxerto osteocondral macerado + IGF-1 (n=5) e enxerto osteocondral macerado + solução fisiológica (n=4). Os animais foram eutanasiados em 12 semanas após a cirurgia e as articulações foram submetidas ao exame de ressonância magnética utilizando um aparelho scanner de 1,5 Tesla de alto campo magnético. Além disso, amostras dos locais de enxertia foram submetidas aos exames anatomopatológicos. O exame de ressonância magnética mostrou--se eficaz como um método não invasivo para avaliação do tecido de reparação em enxertos osteocondrais na cartilagem articular do fêmur de coelhos, fornecendo dados complementares aos exames macroscópicos e histológicos. Por

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Section: Introductionunclassified

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

et al. 2017

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“…MR images were obtained before and 5 min after contrast agent injection (1.0 mL) into the knee joints. A microelement preparation of a high-caloric infusion preparation (Mineric ® Nipro Pharma Corp., Osaka, Japan) was used as the contrast agent [ 23 ]. Mineric ® contains 0.5 mmol/L MnCl 2 , 2.5 mmol/L CuSO 4 , and 17.5 mmol/L FeCl 3 .…”

Section: Methodsmentioning

confidence: 99%

“…The tissues were embedded in paraffin and cut at the location in the same manner as the MR images were selected. The tissues were stained with hematoxylin and eosin (H&E) and Safranin O according to the previous study [ 23 ]. Histological evaluation of OA was performed using the Mankin score and OARSI (Osteoarthritis Research Society International) score of articular cartilage changes in three slices per rabbit [ 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

“…Manganese ions shorten T 2 relaxation times and have been used as components of contrast agents in MRI studies examining the brain and digestive organs [ 20 – 22 ]. In a previous study, the selective visualization of articular cartilage using MRI with a new double-contrast agent containing manganese ions as a positive contrast agent and ferrous and copper ions as negative contrast agents revealed thin-layered structures that were difficult to visualize using conventional methods [ 23 ]. Accordingly, rabbit knee cartilage was successfully and vividly delineated ex vivo in that study.…”

Section: Introductionmentioning

confidence: 99%

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Early detection of osteoarthritis in rabbits using MRI with a double-contrast agent

Onishi

Ikoma

Kido

et al. 2018

BMC Musculoskelet Disord

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BackgroundArticular cartilage degeneration has been evaluated by magnetic resonance imaging (MRI). However, this method has several problems, including its time-consuming nature and the requirement of a high magnetic field or specialized hardware. The purpose of this study was to sequentially assess early degenerative changes in rabbit knee articular cartilage using MRI with a new double-contrast agent.MethodsWe induced osteoarthritis (OA) in the right knee of rabbits by anterior cruciate ligament transection and partial medial meniscectomy. Proton density-weighted images and T2-calculated images were obtained before and after contrast agent injection into the knee. The signal intensity ratio (SIR) values on the proton density-weighted images were calculated by dividing the signal intensity of the articular cartilage by that of joint fluid. Six rabbits were examined using MRI at 2 (designated 2-w OA) and 4 weeks (4-w OA) after the operation. Histological examination was performed 4 weeks after the operation. One rabbit was histologically examined 2 weeks after the operation. The control consisted of six rabbits that were not subjected to the operation. The SIR values, T2 values and the thicknesses of the cartilage of the 2-w OA, 4-w OA and the control before and after contrast agent injection were analyzed. The Mankin score and OARSI (Osteoarthritis Research Society International) score were used for the histological evaluation.ResultsSignificant differences in the SIR and T2 values of the medial and lateral condyles of the femur were found between the control and the 4-w OA only after contrast agent injection. No significant differences were found in the SIR and T2 values before contrast agent injection between the control, the 2-w OA and 4-w OA. The thickness of the articular cartilage revealed no significant differences. In the histological assessment, the Mankin score and OARSI score sequentially increased from the control to the 4-w OA.ConclusionWe evaluated the SIR and T2 values of the knees in a rabbit OA model and a control model using a new double-contrast agent. MRI with this agent enabled OA detection earlier than using conventional MRI.

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Selective visualization of rabbit knee cartilage using MR imaging with a double-contrast agent

Abstract: A clinical trace element mixture was used as a novel double-contrast agent, and it exhibits selective MR contrast in articular cartilage.

Cited by 2 publications

References 31 publications

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

Early detection of osteoarthritis in rabbits using MRI with a double-contrast agent

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