Selenium and mercury bioaccessibility in fish samples: an in vitro digestion method

Cabañero, Ana I.; Madrid, Yolanda; Cámara, Carmen

doi:10.1016/j.aca.2004.09.039

Cited by 185 publications

(89 citation statements)

References 31 publications

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“…The SBF test conditions were set up to be as close to the in vivo situation as possible to simulate the gastric and intestinal digestion process. The solid:liquid ratio was set to 1:100 and the two-stage extraction process was similar to that used by others (Cabanero et al, 2004;Koch et al, 2011) with slight modification (Fig. 1).…”

Section: Sample Collection and Pretreatmentmentioning

confidence: 99%

“…Simulated body fluid (SBF) tests have been used to study the bioaccessibility of several metals in food (Juhasz et al, 2006;Moreda-Pineiro et al, 2011), soil (Kientz et al, 2003), and sediment (Semple et al, 2004), in which the bioaccessible fraction was extracted by using simulated gastric fluid (SGF) or SGF and simulated intestinal fluid (SIF) together. Cabanero et al (2004) have used the SBF test to study the Hg bioaccessibility in fishes. Their results showed that the proportion of bioaccessible Hg in fishes ranged from 9%-17%, depending on the species.…”

Section: Introductionmentioning

confidence: 99%

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Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Yun

Jiang

2016

Journal of Environmental Sciences

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Section: Sample Collection and Pretreatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Yun

Jiang

2016

Journal of Environmental Sciences

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“…The super ata ts ere filtered usi g . The bioaccessibility of Cu, Fe and Mn is defined as the proportion of these elements available for absorption, 29 calculated as follows:…”

Section: Simulated In Vitro Gastric and Gastrointestinal Digestionmentioning

confidence: 99%

Evaluation of in vitro Bioaccessibility of Copper, Iron and Manganese in Amazonian Fruits

Alves¹,

Nunes²,

Dantas³

2017

Rev. Virtual Quim.

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Abstract:The bioaccessibility of Cu, Fe and Mn in Amazonian fruits (açaí, white açaí, bacuri and tucumã) was evaluated in this study using in vitro methods. Flame atomic absorption spectrometry (FAAS) was used for total metal quantification, and graphite furnace atomic absorption spectrometry (GFAAS) was used to quantify the metals in the simulated gastric and gastrointestinal digestions. The Cu concentration in the fruits tested varied between 8.59 and 14.99 mg/kg. For Fe, the concentration in the samples ranged from 35.88 to 60.45 mg/kg. The lowest and highest concentrations of Mn were found in bacuri (5.01 mg/kg) and white açaí (709.93 mg/kg), respectively. In the açaí, white açaí and tucumã samples, the bioaccessible percentage of Cu was higher than the human can absorb. The available Fe value determined in the gastric simulation was similar to that observed in the gastrointestinal simulation, varying from 42.95 to 56.22%. The bioaccessible Mn fraction varied between 28.72 and 68.78% in the gastric simulation and between 26.81 and 64.85% in the gastrointestinal simulation. This study contributes to our knowledge of the actual amount of Cu, Fe and Mn absorbed by ingestion of the studied fruits.Keywords: Bioaccessibility; Amazonian fruits; inorganic elements; FAAS, GFAAS. ResumoA bioacessibilidade de Cu, Fe e Mn em frutas da Amazônia (açaí, açaí branco, bacuri e tucumã) foram avaliadas neste estudo utilizando métodos in vitro. A espectrometria de absorção atômica com chama (FAAS) foi utilizada para a quantificação total de metal e a espectrometria de absorção atômica em forno de grafite (GF AAS) foi utilizada para quantificar os metais nas digestões gástricas e gastrointestinais simuladas. A concentração de Cu nos frutos testados variou entre 8,59-14,99 mg/kg. Para Fe, a concentração nas amostras variou de 35,88-60,45 mg/kg. As menores e maiores concentrações de Mn foram encontradas nos bacuri (5,01 mg/kg) e açaí branco (709,93 mg/kg), respectivamente. Nas amostras de açaí, açaí branco e tucumã, o percentual bioaccessível de Cu foi maior do que o ser humano pode absorver. O valor de Fe disponível na simulação gástrica foi semelhante ao observado na simulação gastrointestinal, variando de 42,95 a 56,22%. A fração Mn bioacessível variou entre 28,72-68,78% na simulação gástrica e 26,81-64,85% na simulação gastrointestinal. Este estudo contribui para o conhecimento da quantidade real de Cu, Fe e Mn absorvidos pela ingestão dos frutos estudados.

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“…Essential micronutrients, such as Ca (Silva et al, 2001), Cu (Miller, Schricker, Rasmussen & Van Campen, 1981), Fe (Miller, Schricker, Rasmussen & Van Campen, 1981;Silva et al, 2001), Mg (Silva et al, 2001), Se (Ponce de Leon, Sutton & Caruso, Uden, 2000) and Zn (Silva, Lopes, Nóbrega, Souza & Nogueira, 2001), or toxic elements, such as As (Pardo-Martínez, Viñas & Fisher, Hill, 2001;Peña-Farfal et al, 2004), Hg (Cabañero, Madrid & Cámara, 2004) and Pb (Azenha & Vasconcelos, 2000), were investigated. Only a few studies were performed using enzymatic extraction procedures for multi-elemental analysis, which can lead to measurements based on the quantification of more than eight different elements (Oomen et al, 2002;Peña-Farfal et al, 2004;Peña-Farfal et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Only a few studies were performed using enzymatic extraction procedures for multi-elemental analysis, which can lead to measurements based on the quantification of more than eight different elements (Oomen et al, 2002;Peña-Farfal et al, 2004;Peña-Farfal et al, 2005). The concentration of both the major and trace elements examined was mainly assessed using spectroscopic methods, in particular FAAS (Azenha & Vasconcelos, 2000), HG-AAS (Pardo-Martínez, Viñas, Fisher & Hill, 2001), AFS (Dernovics, Stefánka & Fodor, 2002;Cabañero, Madrid & Cámara, 2004), ICP-OES (Dernovics, Stefánka & Fodor, 2002;Peña-Farfal et al, 2005, ICP-MS (Casiot, Szpunar, Łobiński & Potin-Gautier, 1999Cabañero, Madrid & Cámara, 2004;Reyes et al, 2006), INNA (Kulkarni, Acharya, Rajurkar & Reddy, 2007).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Bioavailability of Mineral Nutrients in Breakfast Cereals

Leśniewicz¹,

Kretowicz²,

Wierzbicka³

et al. 2012

JFR

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The bioavailability of both micro-and macroelements was investigated under conditions simulating the digestion processes in the human alimentary system. A one-step enzymatic extraction was applied using buffered solutions containing pepsin, trypsin, α-amylase or pancreatin, which are enzymes that hydrolyse different nutritional food components such as peptides, carbohydrates and lipids, as the extractant. Corn flakes and multigrain breakfast cereals containing taste additives from a local market in the Wroclaw agglomeration were selected for study as an important kind of ready-to-eat meal. The most popular brands (corn flakes from Nestlé, Mlekołaki and Hanne as well as wheat-based breakfast cereals from Chocapic, Nesquik and Fitness) were analysed. Microwave digestion was employed for sample preparation, and the total concentrations of both micronutrients (Al, Ca, Cu, Fe, Mg, Mn, P, Sr and Zn) and metals released during enzymatic in vitro digestion were measured by inductively coupled plasma-optical emission spectrometry (ICP-OES). Analysis of a Standard Reference Material was performed to validate the applied analytical procedure. Fractionation of the metals bound to the peptides, lipids and carbohydrates was evaluated and discussed.

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Selenium and mercury bioaccessibility in fish samples: an in vitro digestion method

Cited by 185 publications

References 31 publications

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Evaluation of in vitro Bioaccessibility of Copper, Iron and Manganese in Amazonian Fruits

In Vitro Bioavailability of Mineral Nutrients in Breakfast Cereals

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