Selenium- and Tellurium-Based Antioxidants for Modulating Inflammation and Effects on Osteoblastic Activity

Abstract

Increased oxidative stress plays a significant role in the etiology of bone diseases. Heightened levels of H2O2 disrupt bone homeostasis, leading to greater bone resorption than bone formation. Organochalcogen compounds could act as free radical trapping agents or glutathione peroxidase mimetics, reducing oxidative stress in inflammatory diseases. In this report, we synthesized and screened a library of organoselenium and organotellurium compounds for hydrogen peroxide scavenging activity, using macrophagic cell lines RAW264.7 and THP-1, as well as human mono- and poly-nuclear cells. These cells were stimulated to release H2O2, using phorbol 12-myristate 13-acetate, with and without organochalogens. Released H2O2 was then measured using a chemiluminescent assay over a period of 2 h. The screening identified an organoselenium compound which scavenged H2O2 more effectively than the vitamin E analog, Trolox. We also found that this organoselenium compound protected MC3T3 cells against H2O2-induced toxicity, whereas Trolox did not. The organoselenium compound exhibited no cytotoxicity to the cells and had no deleterious effects on cell proliferation, viability, or alkaline phosphatase activity. The rapidity of H2O2 scavenging and protection suggests that the mechanism of protection is due to the direct scavenging of extracellular H2O2. This compound is a promising modulators of inflammation and could potentially treat diseases involving high levels of oxidative stress.

Antioxidant Activity of Silica-Based Bioactive Glasses

“…Then, the scavenging ability for oxygen/nitrogen radicals (RONS) in the presence of cells was evaluated in the supernatants using a specific kit (In vitro ROS/RNS assay, Cell Biolabs, Inc., San Diego, CA, USA), and the specimens’ ability to preserve the cell viability was evaluated after inducing oxidative stress for 72 h by means of H 2 O 2 (500 mM, 3 h/d) addition into the medium. 22 Results were statistically analyzed using SPSS v25 software (IBM). Groups were compared using the one-way ANOVA test and Tukey’s posthoc analysis.…”

Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress

“…In addition, antioxidant activity was implicated in at least part of the therapeutic properties of organoselenium compounds [39,40]. This effect was further showed in studies performed with cell culture [41][42][43] and animal species such as rats [44,45], fishes [46], Drosophila melanogaster [47], and Caenorhabditis elegans [48].…”

Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress

“…In addition, antioxidant activity was implicated in at least part of therapeutic properties of organoselenium compounds [39][40]. This effect was further showed in studies performed with cell culture [41][42][43] and animal species such as rats [44][45], fishes [46], Drosophila melanogaster [47], and Caenorhabditis elegans [48].…”