Selenium: Biochemical Role As A Component Of Glutathione Peroxidasec

Rotruck, John T.; Pope, A. L.; Ganther, Howard E.; Swanson, Anne; Hafeman, Dean G.; Hoekstra, W. G.

doi:10.1111/j.1753-4887.1980.tb05961.x

Cited by 67 publications

(51 citation statements)

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“…Selenium (Se) is recognized as essential microelement for humans and animals, mainly due to its antioxidative properties and role in hormone balance (Schwartz and Foltz 1957;Flohe et al 1973;Rotruck et al 1973). Moreover, dietary Se concentrations that are either too low or too high can be lethal to humans and animals (Combs 2001).…”

Section: Introductionmentioning

confidence: 99%

Agronomic biofortification of Brassica with selenium—enrichment of SeMet and its identification in Brassica seeds and meal

Seppänen¹,

Kontturi²,

Heras

et al. 2010

Plant Soil

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Selenium (Se) is an essential micronutrient and is circulated to the food chain through crops. Brassica species are efficient in Se accumulation and thus, good species for Se biofortification purposes. The residual fraction obtained after oil processing of Brassica seeds, the meal, is an important protein source in animal diets and used in feed concentrates. The accumulation of soil or foliar applied Se in the seeds and meal of Brassica napus and B. rapa as well as its effects on growth and yield formation was studied in two field experiments. Also, a HPLC-ICP-MS based method for the identification and quantification of Se species in Brassica seeds and meal was developed. Selenium application did not affect the yield or oil content. High accumulation of Se in the seeds and meal (1.92-1.96 μg Se g −1 ) was detected. Biotransformation of inorganic Se was evaluated by using HPLC-ICP-MS previous enzymatic hydrolysis for species extraction. The Se speciation studies showed that up to 85% of the total Se was SeMet whereas other Se-species were not detected. We conclude that the agronomic biofortification of Brassica species can improve the nutritive quality of the protein rich meal fraction as it contains significant amount of SeMet.

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Section: Introductionmentioning

confidence: 99%

Agronomic biofortification of Brassica with selenium—enrichment of SeMet and its identification in Brassica seeds and meal

Seppänen¹,

Kontturi²,

Heras

et al. 2010

Plant Soil

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“…The level of reduced glutathione (GSH) was measured by the method of Moron et al (1979) on the basis of the reaction of 5,5 0 -dithiobis-2-nitrobenzoic acid which is readily reduced by sulfhydryls forming a yellow substance which was measured at 412 nm and expressed as lmoles/mg protein. The enzyme glutathione peroxidase (GPx) was assayed according to the method of Rotruck et al (1973). The assay takes advantage of concomitant oxidation of NADPH by GR, which was measured at 340 nm.…”

Section: Biochemical Determinationsmentioning

confidence: 99%

Protective Effect of Bacopa monniera on Methyl Mercury-Induced Oxidative Stress in Cerebellum of Rats

et al. 2012

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Methyl mercury (MeHg) is a ubiquitous environmental pollutant leading to neurological and developmental deficits in animals and human beings. Bacopa monniera (BM) is a perennial herb and is used as a nerve tonic in Ayurveda, a traditional medicine system in India. The objective of the present study was to investigate whether Bacopa monniera extract (BME) could potentially inhibit MeHg-induced toxicity in the cerebellum of rat brain. Male Wistar rats were administered with MeHg orally at a dose of 5 mg/kg b.w. for 21 days. Experimental rats were given MeHg and also administered with BME (40 mg/kg, orally) for 21 days. After the treatment period, we observed that MeHg exposure significantly inhibited the activities of superoxide dismutase, catalase, glutathione peroxidase, and increased the glutathione reductase activity in cerebellum. It was also found that the level of thiobarbituric acid-reactive substances was increased with the concomitant decrease in the glutathione level in MeHg-induced rats. These alterations were prevented by the administration of BME. Behavioral interference in the MeHg-exposed animals was evident through a marked deficit in the motor performance in the rotarod task, which was completely recovered to control the levels by BME administration. The total mercury content in the cerebellum of MeHg-induced rats was also increased which was measured by atomic absorption spectrometry. The levels of NO(2) (-) and NO(3) (-) in the serum were found to be significantly increased in the MeHg-induced rats, whereas treatment with BME significantly decreased their levels in serum to near normal when compared to MeHg-induced rats. These findings strongly implicate that BM has potential to protect brain from oxidative damage resulting from MeHg-induced neurotoxicity in rat.

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“…Reduced glutathione (GSH) was assayed by the method of Moron et al [26] on the basis of the reaction of 5-5, dithiobis-2-nitrobenzoic acid which is readily reduced by sulfhydryls forming a yellow substance which is measured at 412 nm. The enzyme glutathione peroxidase (GPx) was assayed according to the method of Rotruck et al [27]. The assay takes advantage of concomitant oxidation of NADPH by GR, which is measured at 340 nm.…”

Section: Biochemical Analysismentioning

confidence: 99%

Protective Role of Cynodon dactylon in Ameliorating the Aluminium-Induced Neurotoxicity in Rat Brain Regions

Sumathi

Shobana

Kumari

et al. 2011

Biol Trace Elem Res

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Cynodon dactylon (Poaceae) is a creeping grass used as a traditional ayurvedic medicine in India. Aluminium-induced neurotoxicity is well known and different salts of aluminium have been reported to accelerate damage to biomolecules like lipids, proteins and nucleic acids. The objective of the present study was to investigate whether the aqueous extract of C. dactylon (AECD) could potentially prevent aluminium-induced neurotoxicity in the cerebral cortex, hippocampus and cerebellum of the rat brain. Male albino rats were administered with AlCl(3) at a dose of 4.2 mg/kg/day i.p. for 4 weeks. Experimental rats were given C. dactylon extract in two different doses of 300 mg and 750 mg/keg/day orally 1 h prior to the AlCl(3) administration for 4 weeks. At the end of the experiments, antioxidant status and activities of ATPases in cerebral cortex, hippocampus and cerebellum of rat brain were measured. Aluminium administration significantly decreased the level of GSH and the activities of SOD, GPx, GST, Na(+)/K(+) ATPase, and Mg(2+) ATPase and increased the level of lipid peroxidation (LPO) in all the brain regions when compared with control rats. Pre-treatment with AECD at a dose of 750 mg/kg b.w increased the antioxidant status and activities of membrane-bound enzymes (Na(+)/K(+) ATPase and Mg(2+) ATPase) and also decreased the level of LPO significantly, when compared with aluminium-induced rats. The results of this study indicated that AECD has potential to protect the various brain regions from aluminium-induced neurotoxicity.

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Selenium: Biochemical Role As A Component Of Glutathione Peroxidasec

Cited by 67 publications

References 0 publications

Agronomic biofortification of Brassica with selenium—enrichment of SeMet and its identification in Brassica seeds and meal

Agronomic biofortification of Brassica with selenium—enrichment of SeMet and its identification in Brassica seeds and meal

Protective Effect of Bacopa monniera on Methyl Mercury-Induced Oxidative Stress in Cerebellum of Rats

Protective Role of Cynodon dactylon in Ameliorating the Aluminium-Induced Neurotoxicity in Rat Brain Regions

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