Selenium Determination in Soil and Plant Materials

Abstract

Selenium (Se) was determined in soil and was adjusted to pH 1.8, and a stabili'ing plant sampies after wet digestion with HNO" agent consisting 9f l0V9 _hydroxylamine hyind HCIO.. The melhod included the usuai diochloride in a 0.04 M ED'IA solution was steps, nambly, the reduction of Se, complex-used. Fluorescence measurements obtained ing Se with 1,3-diaminonaphthalene, and the for soil samples gave average coefficie-nts ^of exiraction of'the piazseleriol in cyclohexane variation and recovery perc_entages. of 3.35 followed by fluoroimetric measurements. The and 97.4, respectively. For radioactivity_colnts most impoitant step, the stabilization of sele-made on cyclohexane extracts, the coefficients nium at the oxidative state Se*', was achieved of variation and the recovery Perceltages by reducing Se*6 to Se*t wiih 2 ml of I N were 2.31 and 93.1, respectively. Similar reficl whleltre system was kept in a boiling sults were obtained for plant materials. Maj_or water bath. Foi best resul.ts^ and adequatE problems encountered in establishing the control of interfei"ing ferric ions, the syitem modified method are discussed'

Selenium in storage proteins of wheat cultivated on selenium impacted soils of Punjab, India

“…Selenium was determined in defatted wheat fl our, starch residue (residue left after extraction), and all dry protein fractions using fl uorescence spectrometric method (LEVESQUE & VENDETTE, 1971). This method involved digestion with HNO 3 and HClO 4 , reduction of selenium from Se +6 to Se +4 with HCl, complexing of Se +4 with DAN to form piazselenol and extraction of the piazselenol in cyclohexane.…”

Selenium Uptake by Edible Oyster Mushrooms (Pleurotus sp.) from Selenium-Hyperaccumulated Wheat Straw

“…Selenium content in powdered samples was analysed using a fluorescence spectrometric method (26). In brief, a 100 mg portion of the sample was weighed accurately in an acid-washed kjeldahl flask containing 5 mL concentrated nitric acid.…”

Selenium Content of Soils and Crops and the Effects of Lime and Sulfur on Plant Selenium

“…The objectives of this study were: (1) The soil was analyzed forpH and total Se; the plant tissues and cereals were dried at 80 C, ground, and analyzed for Se by the method of Levesque and Vendette (1971 (NAS-NRC 1971). Liming of soil to raise thepH from 6.0 to 6.9 has been shown to increase the uptake of added Se by alfalfa (Cary et al 1961).…”