Selenium Regulates Gene Expression of Selenoprotein W in Chicken Skeletal Muscle System

Abstract

Selenoprotein W (SelW) is abundantly expressed in skeletal muscles of mammals and necessary for the metabolism of skeletal muscles. However, its expression pattern in skeletal muscle system of birds is still uncovered. Herein, to investigate the distribution of SelW mRNA in chicken skeletal muscle system and its response to different selenium (Se) status, 1-day-old chickens were exposed to various concentrations of Se as sodium selenite in the feed for 35 days. In addition, myoblasts were treated with different concentrations of Se in the medium for 72 h. Then the levels of SelW mRNA in skeletal muscles (wing muscle, pectoral muscle, thigh muscle) and myoblasts were determined on days 1, 15, 25, and 35 and at 0, 24, 48, and 72 h, respectively. The results showed that SelW was detected in all these muscle components and it increased both along with the growth of organism and the differentiation process of myoblasts. The thigh muscle is more responsive to Se intake than the other two skeletal muscle tissues while the optimal Se supplementation for SelW mRNA expression in chicken myoblasts was 10(-7) M. In summary, Se plays important roles in the development of chicken skeletal muscles. To effect optimal SelW gene expression, Se must be provided in the diet and the media in adequate amounts and neither at excessive nor deficient levels.

The supranutritional selenium status alters blood glucose and pancreatic redox homeostasis via a modulated selenotranscriptome in chickens (Gallus gallus)

“…[55][56][57] Prior studies indicated that Se deciency inuenced the expressions of 24 selenoproteins, such as Txnrd1, Sels, Selu, Sepx1, Sepw1 in the immune system, pancreas of chickens. [58][59][60] In the present study, we mainly studied the effect of dietary Se status on the expressions of all 24 selenoproteins in pancreas. The supranutritional Se intake increased mRNA levels of most selenoprotein in the chickens.…”

Effect of 2-hydroxy-4-methylselenobutanoic acid as a dietary selenium supplement to improve the selenium concentration of table eggs1

“…Selenium has been recognized as a nutritional essential trace element that is important in many biological processes in mammals and birds (Holben and Smith, 1999;Surai, 2000). It has a crucial role in embryonic and postnatal development (Surai, 2000;Fortier et al, 2012), immunity, reproduction, antioxidant system (Choct et al, 2004;Juniper et al, 2011), and muscle function (Ruan et al, 2012;Zhang et al, 2012) and is known as a natural antioxidant (Surai, 2002). In poultry as well as in other animal species, Se can be added to diets through its mineral or organic forms, which represent the crucial factor that determines its metabolic fate (Suzuki, 2005).…”

Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues

“…SelW mRNA expression in the gastrointestinal tract, liver, pancreatic tissue; immune organs and skeletal muscle system in chickens are highly sensitive to Se supply (Li et al 2010;Gao et al 2011;Sun et al 2011;Wang et al 2011;Yu et al 2011;Ruan et al 2011). Pagmantidis et al (2005) reported that SelN and SelW expression levels in human intestinal Caco-2 cells is highly sensitive to Se-depletion and Seadequate conditions.…”