Selenium Regulates Gene Expression of Selenoprotein W in Chicken Gastrointestinal Tract

Gao, Xuejiao; Xing, Houjuan; Li, Shu; Li, Jinlong; Ying, Tao; Xu, Shiwen

doi:10.1007/s12011-011-9175-x

Cited by 44 publications

(14 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is evidence from in vitro and in vivo studies that expression of TrxR1, SelP, and SelW mRNA is highly up-regulated by SS supplementation in comparison with Se deficiency (Hill et al 1996;Hadley and Sunde 2001;Pagmantidis et al 2005;Gao et al 2012). However, the regulation of TrxR1, SelP, and SelW mRNA by different Se forms in avian liver, kidney, and muscle is not known.…”

Section: Resultsmentioning

confidence: 99%

Comparison of different forms of dietary selenium supplementation on gene expression of cytoplasmic thioredoxin reductase, selenoprotein P, and selenoprotein W in broilers

Zhan¹,

Wang²,

Yuan³

et al. 2014

Czech J. Anim. Sci.

View full text Add to dashboard Cite

ABSTRACT:Effects of different forms of dietary selenium (Se) supplementation on gene expression of cytoplasmic thioredoxin reductase (TrxR1), selenoprotein P (SelP), and selenoprotein W (SelW) in broilers were investigated. A total of six hundred Ross 308 broilers (1-day-old) with similar body weight were randomly divided into three groups, each of which included 5 replicates of 40 birds. These three treatments received the same basal diet with only background Se level of 0.04 mg Se/kg, supplemented with 0.15 mg Se/kg as sodium selenite (SS) or l-selenomethionine (l-Se-Met) or d-selenomethionine (d-Se-Met) for 42 days. The SS supplemented diet increased TrxR1 activity in liver (P < 0.01) and kidney (P < 0.01) as well as SelP concentration in serum (P < 0.05) and liver (P < 0.01) more than the d-Se-Met supplemented diet. The addition of SS also highly increased liver (P < 0.01) and kidney (P < 0.01) TrxR1 activities of broilers in comparison with broilers fed l-Se-Met diet. In addition, liver TrxR1 activity in l-Se-Met group was higher than that in d-Se-Met group (P < 0.05). Liver and kidney mRNA levels of TrxR1 and SelP as well as breast muscle SelW mRNA level were significantly increased by l-and d-Se-Met supplementation in comparison with SS supplementation (P < 0.01), while the d-Se-Met group showed more effective (P < 0.01) than the l-Se-Met group in increasing the mRNA levels of TrxR1 and SelP in liver and kidney. Therefore, dietary l-Se-Met and d-Se-Met supplementation could improve mRNA levels of different selenoproteins studied and reduce amounts of TrxR1 and SelP in broilers compared with SS. Besides, l-Se-Met is more effective than d-Se-Met in raising TrxR1 activity and decreasing mRNA abundance of TrxR1 and SelP in broilers.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparison of different forms of dietary selenium supplementation on gene expression of cytoplasmic thioredoxin reductase, selenoprotein P, and selenoprotein W in broilers

Zhan¹,

Wang²,

Yuan³

et al. 2014

Czech J. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…Se implements its biological function through incorporation into selenoproteins. In addition, Se deficiency caused injuries in several different chicken organs, including the brain (Sheng et al 2014;Xu et al 2013), muscles (Yao et al 2013a, b), the gastrointestinal tract (Gao et al 2012), the spleen (Yu et al 2011) and the liver (Sun et al 2011). The study of Fischer showed that the liver contains a high concentration of Se, which participates in many biological processes that range from cellular antioxidant defense to the protection and repair of DNA to apoptosis (Fischer et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Roles of oxidative stress and endoplasmic reticulum stress in selenium deficiency-induced apoptosis in chicken liver

Yao

et al. 2015

Biometals

Self Cite

View full text Add to dashboard Cite

Oxidative stress and endoplasmic reticulum (ER) stress are involved in different types of stress-induced injuries. The aim of the present study was to evaluate the effect of Se deficiency on oxidative stress, ER stress and apoptosis in chicken livers. Chickens (1 day old, n = 180) were randomly divided into two groups: the L group [fed with a Se-deficient (Se 0.033 mg/kg) diet] and the control group [fed with a normal (Se 0.2 mg/kg) diet]. Factor-associated oxidative stress, catalase (CAT) activity, H2O2 production and the inhibition of hydroxyl radicals (·OH) in the chicken liver were determined on days 15, 25, 35, 45, 55 and 65, respectively. In addition, ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and apoptosis-related genes (caspase3 and Bcl-2) were examined by fluorescence quantitative PCR or western blot analysis. Apoptosis levels were also measured using ultrastructural observations and the TdT-mediated dUTP nick end labeling assay. The results showed that CAT activity and ·OH inhibition were decreased and that H2O2 production was increased in the low-Se group, which demonstrated that oxidative stress occurred in the chicken liver. The ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and the apoptosis-related gene caspase3 were increased (p < 0.05), while Bcl-2 was decreased (p < 0.05) by Se deficiency. In addition, apoptosis and ER lesions were observed by ultrastructural observations of the chicken liver in the low-Se group. The level of apoptosis and the number of apoptotic cells increased with time. These results indicated that the oxidative-ER stress pathway participates in Se deficiency-induced apoptosis in the chicken liver.

show abstract

“…SelW mRNA expression in the gastrointestinal tract, liver, pancreatic tissue; immune organs and skeletal muscle system in chickens are highly sensitive to Se supply (Li et al 2010;Gao et al 2011;Sun et al 2011;Wang et al 2011;Yu et al 2011;Ruan et al 2011). Pagmantidis et al (2005) reported that SelN and SelW expression levels in human intestinal Caco-2 cells is highly sensitive to Se-depletion and Seadequate conditions.…”

Section: Discussionmentioning

confidence: 99%

Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues

Zhang

Huang

et al. 2011

Biometals

Self Cite

View full text Add to dashboard Cite

Selenium (Se), selenoprotein N (SelN) and selenoprotein W (SelW) play a crucial role in muscle disorders. Se status highly regulates selenoprotein mRNA levels. However, few attempts have been performed on the effect of dietary Se supplementation on muscle SelN and SelW mRNA levels in birds. To investigate the effects of Se on the regulation of SelN and SelW mRNA levels in muscle tissues, one-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0 or 5.0 mg/kg sodium selenite for 90 days. Muscle tissues (breast, flight, thigh, shank and cardiac muscles) were collected and examined for Se content and mRNA levels of SelN and SelW. Moreover, Selenophosphate synthetase-1 (SPS-1) and selenocysteine-synthase (SecS) mRNA levels were analyzed. Significant increases in SelN mRNA levels were obtained in breast, thigh and shank muscles treated with Se, with maximal effects at 3.0 mg Se/kg diet, but 2.0 mg Se/kg diet resulted in peak levels of Sel N mRNA in flight muscles. Changes in SelW mRNA abundance in thigh and shank muscles increased in response to Se supply. After reaching a maximal level, higher Se supplementation led to a reduction in both SelN and SelW mRNAs. However, SelN and SelW mRNA levels displayed a different expression pattern in different skeletal and cardiac muscles. Thus, it suggested that skeletal and cardiac muscles SelN and SelW mRNA levels were highly regulated by Se supplementation and different muscle tissues showed differential sensitivity. Moreover, Se supplementation also regulated the levels of SPS1 and SecS mRNAs. The mRNA levels of SPS1 and SecS were enhanced in the Se supplemented groups. These data indicate that Se regulates the expression of SelN and SelW gene and affect the mRNA levels of SecS and SPS1.

show abstract

Selenium Regulates Gene Expression of Selenoprotein W in Chicken Gastrointestinal Tract

Cited by 44 publications

References 44 publications

Comparison of different forms of dietary selenium supplementation on gene expression of cytoplasmic thioredoxin reductase, selenoprotein P, and selenoprotein W in broilers

Comparison of different forms of dietary selenium supplementation on gene expression of cytoplasmic thioredoxin reductase, selenoprotein P, and selenoprotein W in broilers

Roles of oxidative stress and endoplasmic reticulum stress in selenium deficiency-induced apoptosis in chicken liver

Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues

Contact Info

Product

Resources

About