Selenium Speciation in the Fountain Creek Watershed (Colorado, USA) Correlates with Water Hardness, Ca and Mg Levels

Carsella, James S.; Sánchez-Lombardo, Irma; Bonetti, Sandra J.; Crans, Debbie C.

doi:10.3390/molecules22050708

Cited by 9 publications

(6 citation statements)

References 42 publications

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“…The interpretation of the 51 V NMR spectral data was supplemented by speciation calculations based on constants measured previously (Pettersson et al, 1983 ; Ehde et al, 1989 ; Selling et al, 1994 ). The species distribution diagrams were calculated by using HySS 2009 software (Alderighi et al, 1999 ; Carsella et al, 2017 ) and known speciation constants of the system at hand (Pettersson et al, 1983 ; Ehde et al, 1989 ; Selling et al, 1994 ). The citrate and the phosphate concentrations found in the Middlebrook 7H9 broth medium supplemented with 5% BSA, 2% dextrose, 5% catalase (ADC) enrichment, glycerol (0.2%, v/v), and Tween 80 (0.05%, v/v) were 0.48 and 24 mM, respectively ( Bbl tm , 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

et al. 2018

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51V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of Mycobacterium smegmatis (M. smeg) and Mycobacterium tuberculosis (M. tb) was investigated, and speciation was found to be critical for the observed growth inhibition. Specifically, the large orange-colored sodium decavanadate (V10O286-) anion was found to be a stronger inhibitor of growth of two mycobacterial species than the colorless oxovanadate prepared from sodium metavanadate. The vanadium(V) speciation in the growth media and conversion among species under growth conditions was monitored using 51V NMR spectroscopy and speciation calculations. The findings presented in this work is particularly important in considering the many applications of polyoxometalates in biological and medical studies, such as the investigation of the phase-problem in X-ray crystallography for the ribosome. The findings presented in this work investigate the interactions of oxometalates with other biological systems.

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Section: Methodsmentioning

confidence: 99%

Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

et al. 2018

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“…It is noted that almost all the concentrations of TSe-W in the wild areas were higher than those in the aquaculture areas, indicating that human activities have important effects on the concentration of selenium in fishpond environments. As limited by the sensitivity of HPLC-HG-AFS, no Se(IV) and Se(VI) were detectable in water, but numerous studies have revealed that Se(VI) is the main existing form in natural water (Carsella, Sanchez-Lombardo, Bonetti, & Crans, 2017;Serafimovska, Arpadjan, Stafilov, & Popov, 2011). Total mercury in water varied within 0.36 to 0.55 μg/L with an average value of 0.49 μg/L for the wild areas and 0.42 μg/L for the aquaculture areas.…”

Section: Species and Concentrations Of Selenium And Mercury In Watermentioning

confidence: 99%

Species and correlations between selenium and mercury in fishpond ecosystems

Wang

Nan

et al. 2019

Water Environment Research

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The chemical species and contents of selenium and mercury in water, sediments, and crucian carps collected from three wild and three aquaculture fishponds in Tianjin, China, were determined, and the interaction between selenium and mercury in water was also investigated by the calorimetry method. The results revealed that the average contents of total selenium (TSe) and total mercury (THg) in each item of the wild areas were higher than in those of the aquaculture areas, and significant differences (95% confidence) were presented for THg both in the sediments and crucian carps. The molar ratios between TSe and THg in all investigated fishponds were far higher than 1, indicating good protective effects of selenium on mercury toxicity. Obviously, negative correlations (r > 0.9993) were found between TSe and THg in water. The antagonism of selenium on mercury in water was confirmed to mainly result from the reaction between selenate and Hg2+ to form an insoluble selenium–mercury oxygenated compound, by which the addition of selenate into the water of fishponds would reduce the environmental risk of mercury. Practitioner points Se and Hg in different fishpond ecosystems were investigated and compared. Direct evidence was provided for the interaction between Se and Hg in water. The addition of Se(VI) into fishpond ecosystems would reduce the environmental risk of Hg.

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“…The environmental levels of selenium (Se) are regulated and strictly controlled in the USA by the Environmental Protection Agency (EPA). The correlation between selenium and other elements is also important [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

et al. 2020

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A fit for purpose analytical protocol was designed towards searching for low molecular weight seleno-compounds in sprouts. Complementary analytical techniques were used to collect information enabling the characterization of selenium speciation. Conceiving the overall characterization of the behavior of selenium, inductively plasma optical mass spectrometry (ICP-MS) was used to determine the total selenium content in entire sprouts as well as in selected extracts or chromatographic fractions. Then, high-performance liquid chromatography combined with ICP-MS (HPLC-ICP-MS) was used to evaluate the presence of inorganic and organic seleno-compounds, with the advantages of being very sensitive towards selenium, but limited by available selenium standard compounds. Finally, ultra-high performance liquid chromatography electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS/MS) and UHPLC-ESI-Orbitrap-MS/MS were used for the confirmation of the identity of selected compounds and identification of several unknown compounds of selenium in vegetable sprouts (sunflower, onion, radish), respectively. Cultivation of plants was designed to supplement sprouts with selenium by using solutions of selenium (IV) at the concentration of 10, 20, 40, and 60 mg/L. The applied methodology allowed to justify that vegetable sprouts metabolize inorganic selenium to a number of organic derivatives, such as seleno-methylselenocysteine (SeMetSeCys), selenomethionine (SeMet), 5′-seleno-adenosine, 2,3-DHP-selenolanthionine, Se-S conjugate of cysteine-selenoglutathione, 2,3-DHP-selenocysteine-cysteine, 2,3-DHP-selenocysteine-cysteinealanine, glutathione-2,3-DHP-selenocysteine, gamma-Glu-MetSeCys or glutamyl-glycinyl-N-2,3-DHP-selenocysteine.

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Selenium Speciation in the Fountain Creek Watershed (Colorado, USA) Correlates with Water Hardness, Ca and Mg Levels

Cited by 9 publications

References 42 publications

Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

Species and correlations between selenium and mercury in fishpond ecosystems

Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

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