Selenium-substituted hydroxyapatite nanoparticles and their in vivo antitumor effect on hepatocellular carcinoma

Wang, Yanhua; Hao, Hang; Li, Yan; Zhang, Shengmin

doi:10.1016/j.colsurfb.2015.12.056

Cited by 85 publications

(41 citation statements)

References 51 publications

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“…HA doped with selenium ions can be obtained in two ways: through synthesis, during which ions are incorporated into the HA structure [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47], or by ion exchange, where soaking in a selenium salt solution results in the exchange of ions in the HA structure [47].…”

Section: Synthesis Of Hydroxyapatites Doped With Selenite and Selenatmentioning

confidence: 99%

“…The obtained powders contained various amounts of selenium [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47], most often up to 10% by weight [33,36,37,40,41,43,44,46]. It should be noted that the reaction efficiency was often lower than 100% [33,36,40,41,43,44,46].…”

Section: Synthesis Of Hydroxyapatites Doped With Selenite and Selenatmentioning

confidence: 99%

“…It is worth noting that the SeO 3 -HA obtained in the studies [34,38,39] was used to prepare biocomposites containing silk fibres [34] or lysozyme [39]. It was possible to incorporate between 6 and 30% of selenium ions relative to phosphorus ions into such materials.…”

Section: Synthesis Of Hydroxyapatites Doped With Selenite and Selenatmentioning

confidence: 99%

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Selenium-Doped Hydroxyapatite Nanocrystals–Synthesis, Physicochemical Properties and Biological Significance

et al. 2018

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Hydroxyapatites (HAs), as materials with a similar structure to bone minerals, play a key role in biomaterials engineering. They have been applied as bone substitute materials and as coatings for metallic implants, which facilitates their osseointegration. One of the beneficial characteristics of HA, when used to create biocompatible materials with improved physicochemical or biological properties, is its capacity for ionic substitution. The aim of the study was to present the current state of knowledge about HAs containing selenate ions IV or VI. The enrichment of HAs with selenium aims to create a material with advantageous effects on bone tissue metabolism, as well as having anticancer and antibacterial activity. The work is devoted to both methods of obtaining Se-HA and an evaluation of its chemical structure and physicochemical properties. In addition, the biological activity of such materials in vitro and in vivo is discussed.Pure, stoichiometric HA crystallizes in a monoclinic system, space group P2 1 /b [5]. However, it is worth mentioning that HA like this is rare and very difficult to obtain by synthesis.

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Section: Synthesis Of Hydroxyapatites Doped With Selenite and Selenatmentioning

confidence: 99%

Section: Synthesis Of Hydroxyapatites Doped With Selenite and Selenatmentioning

confidence: 99%

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Selenium-Doped Hydroxyapatite Nanocrystals–Synthesis, Physicochemical Properties and Biological Significance

et al. 2018

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“…An immediate benefit of this approach would be reduced dependence of patients on strong antibiotics for prophylactic purposes. The incorporation of SeO 3 2− into HAp has also been demonstrated to have a therapeutic effect against hepatocellular carcinoma in vivo 45 . Specifically, while the tumor volume and mass failed to be reduced four weeks after the intravenous injection of Se-HAp, the survival rate of treated vs. untreated mice differed significantly.…”

Section: Introductionmentioning

confidence: 99%

One ion to rule them all: the combined antibacterial, osteoinductive and anticancer properties of selenite-incorporated hydroxyapatite

Uskoković

Iyer

2017

J. Mater. Chem. B

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Although hydroxyapatite (HAp) has been doped with dozens of different ions, the quest for an ion imparting a combination of properties conducive to bone healing is still ongoing. Because of its protean potency and the similarity in size and shape to the phosphate tetrahedron, selenite ion presents a natural ionic substitute in HAp. The incorporation of selenite into synthetic HAp using two different methods – co-precipitation and ion-exchange sorption - was studied for its effect on crystal properties and on a triad of biological responses: antibacterial, anticancer and osteoinductive. Co-precipitation yielded HAp with higher selenite contents than sorption and the stoichiometry of HAp richest in selenite was represented as Ca9.75(PO4)5.75(SeO3)0.25(OH)1.75. Crystallinity of HAp decreased in direct proportion with the amount of selenite incorporated. Because of their lower selenite content, HAp powders prepared by ion-exchange exhibited a consistently higher crystallinity compared to the co-precipitated ones. Annealing partially recovered the crystallinity, yet the difference in crystallinity between powders prepared by co-precipitation and by ion-exchange remained, suggesting that the amorphization is mainly due to structural incorporation of selenite, not its effect on the crystal growth kinetics. The addition of selenite changed the morphology of HAp nanoparticles from acicular to rounded and affected the crystal lattice parameters in different ways depending on whether the powders were annealed or not. As for the annealed powders, the incorporation of selenite contracted the lattice in both a and c crystallographic directions. In the agar diffusion assay, the effectiveness of HAp was more dependent on the presence or absence of selenite in it than on its concentration and was highest against E. coli and S. aureus, moderately high against S. enteritidis and ineffective against P. aeruginosa. In liquid inoculation tests, on the other hand, the antibacterial activity of HAp was directly proportional to the amount of selenite contained in it. The viability of K7M2 osteosarcoma cells decreased in direct proportion with the amount of selenite in HAp and was significantly different from the untreated control and from pure HAp at contents equal to or higher than 1.9 wt.%. In contrast, no reduction was observed in the viability of primary fibroblasts treated with HAp incorporating different amounts of selenite ions, suggesting their potentially selective anticancer activity: lethal for the cancer cells and harmless for the healthy cells. Finally, mRNA expression of bone gamma-carboxyglutamate protein (BGLAP3) was higher in differentiated MC3T3-E1 osteoblastic cells treated with selenite-incorporated HAp particles than in cells treated with pure HAp. The osteoinductive effect was due to an overall higher metabolic activity of cells treated with the particles and not due to increased proliferation. In such a way, a triad of antibacterial, osteoinductive and anticancer activities was attributed to selenite-incorporated ...

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“…As for the choice of the carrier, HAp nanoparticles have been used with success to target tumors through vasculature and extend life expectancy in animal studies. For example, HAp nanoparticles, albeit doped with selenite ions and injected into the bloodstream, extended the lifetime of mice with hepatocellular carcinoma [34]. Also, a direct injection of HAp nanoparticles into a transplanted tumor formed by Bel-7402 human hepatocarcinoma cells in nude mice led to a 50 % reduction in the tumor size [35].…”

Section: Introductionmentioning

confidence: 99%

Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor

Ignjatović

Penov-Gaši

et al. 2016

Colloids and Surfaces B: Biointerfaces

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In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50 = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.

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Selenium-substituted hydroxyapatite nanoparticles and their in vivo antitumor effect on hepatocellular carcinoma

Cited by 85 publications

References 51 publications

Selenium-Doped Hydroxyapatite Nanocrystals–Synthesis, Physicochemical Properties and Biological Significance

Selenium-Doped Hydroxyapatite Nanocrystals–Synthesis, Physicochemical Properties and Biological Significance

One ion to rule them all: the combined antibacterial, osteoinductive and anticancer properties of selenite-incorporated hydroxyapatite

Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor

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