Seleno-Chitosan induces apoptosis of lung cancer cell line SPC-A-1 via Fas/FasL pathway

Wu, Peng; Yu, Shasha; Liu, Chao; Liu, Anjun

doi:10.1016/j.bioorg.2020.103701

Cited by 16 publications

(6 citation statements)

References 42 publications

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“…Captivatingly, and to the best of our knowledge, there have been no previous studies evaluating the apoptotic and necrotic effects of Ps in human breast and lung cancers and comparing it to a nanocarrier loading Ps in which Ps was entrapped in chitosan-coated bilosomal nanoformulation (Ps-CS/BLs). In addition, our findings align with previous studies that reported the chitosan effects in inducing apoptosis mainly by activating caspase-3 [ 58 ] or FAS/FAS-L [ 59 ].…”

Section: Resultssupporting

confidence: 92%

Oral Delivery of Psoralidin by Mucoadhesive Surface-Modified Bilosomes Showed Boosted Apoptotic and Necrotic Effects against Breast and Lung Cancer Cells

Youness¹,

Al-mahallawi

Mahmoud³

et al. 2023

Polymers

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This study aims to design and optimize chitosan-coated bilosomal formulations loaded with psoralidin (Ps-CS/BLs) with improved physicochemical properties, oral bioavailability, and boosted apoptotic and necrotic effects. In this regard, uncoated bilosomes loaded with Ps (Ps/BLs) were nanoformulated using the thin-film hydration technique using different molar ratios of phosphatidylcholine (PC), cholesterol (Ch), Span 60 (S60), and sodium deoxycholate (SDC) (1:0.4:0.2:0.125, 1:0.4:0.2:0.25, and 1:0.4:0.2:0.5, respectively). The best-optimized formulation with respect to size, PDI, zeta potential, and EE% was selected and then coated with chitosan at two different concentrations (0.125 and 0.25 w/v%), forming Ps-CS/BLs. The optimized Ps/BLs and Ps-CS/BLs showed a spherical shape and relatively homogenous size with negligible apparent agglomerations. Additionally, it was demonstrated that coating Ps/BLs with chitosan has significantly increased the particle size from 123.16 ± 6.90 in the case of Ps/BLs to 183.90 ± 15.93 nm in the case of Ps-CS/BLs. In addition, Ps-CS/BLs exhibited higher zeta potential (+30.78 ± 1.44 mV) as compared to Ps/BLs (−18.59 ± 2.13 mV). Furthermore, Ps-CS/BL showed enhanced entrapment efficiency (EE%) of 92.15 ± 7.20% as compared to Ps/BLs (68.90 ± 5.95%). Moreover, Ps-CS/BLs exhibited a more sustained release behavior of Ps compared to Ps/BLs over 48 h, and both formulations were best obeying the Higuchi diffusion model. More importantly, Ps-CS/BLs displayed the highest mucoadhesive efficiency% (74.89 ± 3.5%) as compared to Ps/BLs (26.78 ± 2.9%), indicating the ability of the designed nanoformulation to improve oral bioavailability and extend the residence time inside the gastrointestinal tract upon oral administration. Moreover, upon evaluating the apoptotic and necrotic effects of free Ps and Ps-CS/BLs on human breast cancer cell lines (MCF-7) and human lung adenocarcinoma cell lines (A549), there was a dramatic increase in the percentages of the apoptotic and necrotic cell compared to the control and free Ps. Our findings suggest the possible oral use of Ps-CS/BLs in hampering breast and lung cancers.

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Section: Resultssupporting

confidence: 92%

Oral Delivery of Psoralidin by Mucoadhesive Surface-Modified Bilosomes Showed Boosted Apoptotic and Necrotic Effects against Breast and Lung Cancer Cells

Youness¹,

Al-mahallawi

Mahmoud³

et al. 2023

Polymers

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show abstract

“…Too many Fas/FasL pathway associated proteins were expressed abnormally in cancer ( Gao et al, 2020 ). Chitosan may regulate the expression of apoptosis associated proteins, subsequently activated the cleavage of caspase-9 and caspase-3, which finally induced apoptosis in mitochondrial pathway ( Wu et al, 2018 ; Wu et al, 2020 ).…”

Section: Chitosan: Source Structure Physicochemical Properties and Ap...mentioning

confidence: 99%

Recent Advances in Chitosan and its Derivatives in Cancer Treatment

Ding

Guo

2022

Front. Pharmacol.

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Cancer has become a main public health issue globally. The conventional treatment measures for cancer include surgery, radiotherapy and chemotherapy. Among the various available treatment measures, chemotherapy is still one of the most important treatments for most cancer patients. However, chemotherapy for most cancers still faces many problems associated with a lot of adverse effects, which limit its therapeutic potency, low survival quality and discount cancer prognosis. In order to decrease these side effects and improve treatment effectiveness and patient’s compliance, more targeted treatments are needed. Sustainable and controlled deliveries of drugs with controllable toxicities are expected to address these hurdles. Chitosan is the second most abundant natural polysaccharide, which has excellent biocompatibility and notable antitumor activity. Its biodegradability, biocompatibility, biodistribution, nontoxicity and immunogenicity free have made chitosan become a widely used polymer in the pharmacology, especially in oncotherapy. Here, we make a brief review of the main achievements in chitosan and its derivatives in pharmacology with a special focus on their agents delivery applications, immunomodulation, signal pathway modulation and antitumor activity to highlight their role in cancer treatment. Despite a large number of successful studies, the commercialization of chitosan copolymers is still a big challenge. The further development of polymerization technology may satisfy the unmet medical needs.

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“…This fluorescent dye, when sequestered by active mitochondria of normal cells, revealed yellow-green fluorescence . When the integrity of the mitochondrial membrane is damaged, the permeability transporter pore of the mitochondrial membrane opens, and rhodamine 123 is released . The results that are presented in Figure A,B show that compared with control cells, PSMA-Tyr-treated cells presented a similar green fluorescence intensity (100%).…”

Section: Resultsmentioning

confidence: 78%

“…56 When the integrity of the mitochondrial membrane is damaged, the permeability transporter pore of the mitochondrial membrane opens, and rhodamine 123 is released. 57 The results that are presented in Figure 10A,B show that compared with control cells, PSMA-Tyr-treated cells presented a similar green fluorescence intensity (100%). In contrast, cells treated with PSMA-Gln (74%), PSMA-Phe (49%), or PSMA-Met (46%), showed significant differences in fluorescence intensity, which reflects a loss of mitochondrial membrane potential at 500 μg/mL.…”

Section: Surface Tension Measurementsmentioning

confidence: 79%

Conformational Changes of Poly(Maleic Anhydride-alt-styrene) Modified with Amino Acids in an Aqueous Medium and Their Effect on Cytocompatibility and Hemolytic Response

Maine,

Tamayo,

Leiva

et al. 2023

ACS Appl. Bio Mater.

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Seleno-Chitosan induces apoptosis of lung cancer cell line SPC-A-1 via Fas/FasL pathway

Cited by 16 publications

References 42 publications

Oral Delivery of Psoralidin by Mucoadhesive Surface-Modified Bilosomes Showed Boosted Apoptotic and Necrotic Effects against Breast and Lung Cancer Cells

Oral Delivery of Psoralidin by Mucoadhesive Surface-Modified Bilosomes Showed Boosted Apoptotic and Necrotic Effects against Breast and Lung Cancer Cells

Recent Advances in Chitosan and its Derivatives in Cancer Treatment

Conformational Changes of Poly(Maleic Anhydride-alt-styrene) Modified with Amino Acids in an Aqueous Medium and Their Effect on Cytocompatibility and Hemolytic Response

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