Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer

Salahi, Armita; Varhue, Walter; Farmehini, Vahid; Hyler, Alexandra R.; Schmelz, Eva M.; Davalos, Rafael V.

doi:10.1007/s00216-020-02667-9

Cited by 11 publications

(11 citation statements)

References 38 publications

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“…To compare traditional buffer to Cyto Buffer, some of the most sensitive cell lines (MCF10A 3T3, and DLD1) and more resistant lines (MCF7, MOSE-L TICv ) were exposed to both buffers and native media for the same 3 h time course as above. After exposure, cells were resuspended in native media, re-seeded, and grown for 48 h after which viability was again determined using an MTT assay [24,30,32,37,39,[52][53][54][65][66][67][68][69][70][71][72].…”

Section: Cyto Buffer Formulation Achieves Improved Physiologically Relevant Parameters With a Significantly Improved Viabilitymentioning

confidence: 99%

“…These crossover frequencies are unique to each cell line with the first crossover frequency being dependent on properties external to the cell like membrane morphology and cell size whereas the second crossover frequency is dependent on properties internal to the cell like the cytoplasm and nuclear size [91]. In this work, we are most interested in the first crossover frequency and positive DEP since we are targeting separations based on cell membrane phenotype, as per prior work [37,58,68]. As seen in Fig.…”

Section: Cyto Buffer Minimizes Changes and Noise In Dielectric Spectra And Associated Dielectric Properties Of Bulk Cell Samplesmentioning

confidence: 99%

“…Cells are usually transferred into a buffer consisting of approximately 10% sucrose and <1% glucose in order to achieve ultralow conductivity while attempting to maintain osmolality [24,37,[52][53][54][65][66][67][68]. Some also use low concentration HEPES solutions or PBS supplemented solutions to achieve the same ultralow conductivity [30,39,[68][69][70]. A few groups have added low concentrations of supplements to these base solutions to help in improving cell viability or diminish cell adhesion such as <10% native media, BSA, and EDTA [24,32,37,65,71,72].…”

Section: Introductionmentioning

confidence: 99%

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A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency

Hyler¹,

Hong²,

Davalos

et al. 2021

Electrophoresis

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Cell separation has become a critical diagnostic, research, and treatment tool for personalized medicine. Despite significant advances in cell separation, most widely used applications require the use of multiple, expensive antibodies to known markers in order to identify subpopulations of cells for separation. Dielectrophoresis (DEP) provides a biophysical separation technique that can target cell subpopulations based on phenotype without labels and return native cells for downstream analysis. One challenge in employing any DEP device is the sample being separated must be transferred into an ultralow conductivity medium, which can be detrimental in retaining cells' native phenotypes for separation. Here, we measured properties of traditional DEP reagents and determined that after just 1-2 h of exposure and subsequent culture, cells' viability was significantly reduced below 50%. We developed and tested a novel buffer (Cyto Buffer) that achieved 6 weeks of stable shelf-life and demonstrated significantly improved viability and physiological properties. We then determined the impact of Cyto Buffer on cells' dielectric properties and morphology and found that cells retained properties more similar to that of their native media. Finally, we vetted Cyto Buffer's usability on a cell separation platform (Cyto R1) to determine combined efficacy for cell separations. Here, more than 80% of cells from different cell lines were recovered and were determined to be >70% viable following exposure to Cyto Buffer, flow stimulation, electromanipulation, and downstream collection and growth. The developed buffer demonstrated improved opportunities for electrical cell manipulation, enrichment, and recovery for next generation cell separations.

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Section: Cyto Buffer Formulation Achieves Improved Physiologically Relevant Parameters With a Significantly Improved Viabilitymentioning

confidence: 99%

Section: Cyto Buffer Minimizes Changes and Noise In Dielectric Spectra And Associated Dielectric Properties Of Bulk Cell Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency

Hyler¹,

Hong²,

Davalos

et al. 2021

Electrophoresis

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“…These insulating structures can be as varied in geometry as one wishes. During the last two decades, iDEP techniques have proliferated and evolved, introducing many subclassifications such as reservoir‐based DEP [28], wall‐induced DEP [29], gradient insulator‐based DEP (g‐iDEP) [30], curvature‐induced DEP (c‐iDEP) [31], contactless DEP (cDEP) [32], and others that will surely arise in the future. The main advantage of iDEP is the simplicity to design and fabricate low‐cost devices [6].…”

Section: Dielectrophoretic Systemsmentioning

confidence: 99%

“…5C). While the previous studies separated similar cells, a more recent study, using only one population of mouse ovarian surface epithelial cells, determined the trapping voltage to be 150 V rms at 450 kHz [32]. One of the challenges found in the development of cDEP devices is their fabrication.…”

Section: Voltage Reduction Strategies In Insulator‐based Devicesmentioning

confidence: 99%

Toward low‐voltage dielectrophoresis‐based microfluidic systems: A review

2020

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Dielectrophoretically driven microfluidic devices have demonstrated great applicability in biomedical engineering, diagnostic medicine, and biological research. One of the potential fields of application for this technology is in point‐of‐care (POC) devices, ideally allowing for portable, fully integrated, easy to use, low‐cost diagnostic platforms. Two main approaches exist to induce dielectrophoresis (DEP) on suspended particles, that is, electrode‐based DEP and insulator‐based DEP, each featuring different advantages and disadvantages. However, a shared concern lies in the input voltage used to generate the electric field necessary for DEP to take place. Therefore, input voltage can determine portability of a microfluidic device. This review outlines the recent advances in reducing stimulation voltage requirements in DEP‐driven microfluidics.

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A Review of Cyclic Olefin Copolymer Applications in Microfluidics and Microdevices

Agha

Waheed

Alamoodi

et al. 2022

Macro Materials & Eng

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Cyclic olefin copolymers (COC) are amorphous, transparent thermoplastics composed of cyclic olefin monomers (norbornene) and linear olefins (ethene). They are increasingly utilized as fabrication materials for microsystems and microfluidic devices, owing to their promising features of low water absorption, high electrical insulation, long-term stability of surface treatments, and resistance to a broad variety of acids and solvents. Many manufacturing processes for COC-based devices have been developed in recent decades. These methodologies are categorized as replication methods or fast prototyping as common in fabrication of thermoplastic microfluidic devices. This review gives a full discussion of the features of COCs, the various production processes, and the numerous selected applications in microfluidic platforms. The review also explores COC's composition and fundamental features, as well as fabrication processes and applications in a variety of fields, investigates the material's potential advantages and uses, and attempts to create a comprehensive list of COC's possible benefits. Due to their unique features and simplicity of fabrication, COCs are projected to advance the future of microfluidics, microsystems, and optofluidics.

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Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer

Cited by 11 publications

References 38 publications

A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency

A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency

Toward low‐voltage dielectrophoresis‐based microfluidic systems: A review

A Review of Cyclic Olefin Copolymer Applications in Microfluidics and Microdevices

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