Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging

Song, Juan; Mou, Han-Zhang; Li, Xiao-Qiong; Liu, Yu; Yang, Xianhui; Chen, Hong‐Yuan; Xu, Jing‐Juan

doi:10.1016/j.snb.2022.131644

Cited by 18 publications

(9 citation statements)

References 37 publications

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“…However, the process of modification is time-consuming, complex, and inefficient. With the vigorous development of DNA nanotechnology, plenty of soft selfassembly nanoparticles such as DNA nanospheres (DSs) 17,18 have been employed as nanocarriers to construct versatile DNA nanomachines recently. Compared with hard nano-carriers, functional oligonucleotides can be readily fastened to the soft DNA nanocarrier via hybridization, thereby holding more excellent potential in intracellular biosensing 19−21 drawing upon the inherent biocompatibility and programmability of DNA.…”

Section: ■ Introductionmentioning

confidence: 99%

Endogenous Adenosine Triphosphate-Assisted Three-Dimensional DNA Walker Assembled on Soft Nanoparticles for Intracellular MicroRNA Imaging

et al. 2023

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DNA walkers, a sophisticated type of nanomachines, exhibit intelligent application in biosensing with high programmability and flexibility but usually need additional auxiliary driving force, particularly when walking on hard surfaces. Herein, we construct a three-dimensional (3D) DNA walker on the soft surface of DNA nanospheres (DSs) by using a single-stranded DNA (ssDNA), which is powered by endogenous adenosine triphosphate (ATP) of live cells, so as to sensitively image microRNA (miRNA) in the tumor microenvironment. When the DS walker enters into live cells, miR-21, a general overexpressed biomarker in cancer cells, binds with the blocking strand (B), releasing the walking strand (W) and triggering an ATPpropelled walking reaction. The walking of the DS walker then generates an increasing Cy3 fluorescence signal that indicates the content of miR-21 with about 2.73-fold increase in sensitivity and about 157-fold decrease in the detection limit. Notably, the assembly of the DS walker on soft nanoparticles needs just an easy hybridization process, which facilitates the operation. Meanwhile, this endogenous ATP-powered 3D DNA walker walking on the soft surface performs real-time in situ imaging of miR-21 in live cells, which not only avoids the complex cell treatment and signal error induced by additional auxiliary factors, but also shows high promise of designing programmable DNA nanomachines.

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Section: ■ Introductionmentioning

confidence: 99%

Endogenous Adenosine Triphosphate-Assisted Three-Dimensional DNA Walker Assembled on Soft Nanoparticles for Intracellular MicroRNA Imaging

et al. 2023

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“…When a trigger strand is added to the system, multiple hairpins are triggered in turn and then assembled. It is worth noting that the locked DNA domains with special sequences in the hairpin can be constructed into functional nucleic acid structures by CHAs [ 39 ]. Among them, aptamer [ 40 ], Mg 2+ -ion-dependent DNAzyme [ 41 ], and hemin/G-quadruplex DNAzyme [ 42 ], as functional nucleic acids combined with CHAs, have been used in logical calculations [ 43 ], molecular detection [ 44 , 45 , 46 ], and biosensing [ 47 , 48 ].…”

Section: Introductionmentioning

confidence: 99%

Programming DNA Reaction Networks Using Allosteric DNA Hairpins

et al. 2023

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The construction of DNA reaction networks with complex functions using various methods has been an important research topic in recent years. Whether the DNA reaction network can perform complex tasks and be recycled directly affects the performance of the reaction network. Therefore, it is very important to design and implement a DNA reaction network capable of multiple tasks and reversible regulation. In this paper, the hairpin allosteric method was used to complete the assembly task of different functional nucleic acids. In addition, information conversion of the network was realized. In this network, multiple hairpins were assembled into nucleic acid structures with different functions to achieve different output information through the cyclic use of trigger strands. A method of single-input dual-output information conversion was proposed. Finally, the network with signal amplification and reversible regulation was constructed. In this study, the reversible regulation of different functional nucleic acids in the same network was realized, which shows the potential of this network in terms of programmability and provides new ideas for constructing complex and multifunctional DNA reaction networks.

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“…Delivery vectors are indispensable for realizing detection of target RNAs in living cells . Up to now, various types of nanomaterials have been reported for mediating intracellular RNA detection. , The typical examples are Au nanoparticles, , quantum dots, silicon nanoparticles, MnO 2 nanosheets, MoS 2 nanosheets, carbon nitride nanosheets, graphene, Lipofectamine 3000, metal–organic frameworks, , biomacromolecules, , nucleic acid-based nanostructures, − and hybrid materials. − In addition, modifications of vectors by peptides, , aptamers, , and other functional components to improve the delivery efficiency have been reported . Among various vectors, biomacromolecule and nucleic acid-based vectors with good biocompatibility were confirmed to be able to avoid the interference of the vectors on the target RNA level and thus to ensure the detection accuracy.…”

Section: Introductionmentioning

confidence: 99%

Aptamer/Peptide-Functionalized Nanoprobe for Detecting Multiple miRNAs in Circulating Malignant Cells to Study Tumor Heterogeneity

Han,

Ren,

et al. 2023

ACS Biomater. Sci. Eng.

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Identification of diverse biomarkers in heterogenic circulating malignant cells (CMCs) such as circulating tumor cells (CTCs) and circulating tumor endothelial cells (CTECs) has crucial significance in tumor diagnosis. However, it remains a substantial challenge to achieve in situ detection of multiple miRNA markers in living cells in blood. Herein, we demonstrate that an aptamer/peptidefunctionalized vector can deliver molecular beacons into targeted living CMCs in peripheral blood of patients for in situ detection of multiple cancer biomarkers, including miRNA-21 (miR-21) and miRNA-221 (miR-221). Based on miR-21 and miR-221 levels, heterogenic CMCs are identified for both nondistant metastatic and distant metastatic cancer patients. CMCs from nondistant metastatic and distant metastatic cancer patients exhibit similar miR-21 levels, while the miR-221 level in CMCs of the distant metastatic cancer patient is higher than that of the nondistant metastatic cancer patient. With the capability to realize precise probing of multiple intracellular biomarkers in living CMCs at the single-cell resolution, the nanoprobe can reveal the tumor heterogeneity and provide useful information for diagnosis and prognosis. The nanoprobe we developed would accelerate the progress toward noninvasive precise cancer diagnosis.

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Self-assembled DNA/RNA nanospheres with cascade signal amplification for intracellular MicroRNA imaging

Cited by 18 publications

References 37 publications

Endogenous Adenosine Triphosphate-Assisted Three-Dimensional DNA Walker Assembled on Soft Nanoparticles for Intracellular MicroRNA Imaging

Endogenous Adenosine Triphosphate-Assisted Three-Dimensional DNA Walker Assembled on Soft Nanoparticles for Intracellular MicroRNA Imaging

Programming DNA Reaction Networks Using Allosteric DNA Hairpins

Aptamer/Peptide-Functionalized Nanoprobe for Detecting Multiple miRNAs in Circulating Malignant Cells to Study Tumor Heterogeneity

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