Self‐assembled monolayer for low density lipoprotein detection

Abstract

Heparin (HEP) has been covalently immobilized onto 4-aminothiophenol (ATP) self-assembled monolayer (SAM) deposited onto gold (Au)-coated glass plate for low density lipoprotein (LDL) detection. The HEP/ATP/Au and LDL/HEP/ATP/Au electrodes have been characterized using cyclic voltammetry (CV) and scanning electron microscopy (SEM). Surface plasmon resonance (SPR) measurements reveal that HEP/ATP/Au electrode is sensitive to detection of the LDL in the range 0.03 microM (10 mg/dl)-0.39 microM (130 mg/dl). The values of association and dissociation rate constants in the association phase calculated by kinetic analysis have been found to be k(a) = 9.67 x 10(1) M(-1) s(-1) and k(d) = 2.64 x 10(-4) s(-1).

Low Density Lipoprotein Detection Based on Antibody Immobilized Self-Assembled Monolayer: Investigations of Kinetic and Thermodynamic Properties

“…33,34 The sensitivity calculated from the calibration curve for the AAB/ATP/Au immunosensor was found to be 977.96 m°µM -1 , which is greater than that obtained in the recently reported HEP/ ATP/Au sensor. 16 The influence of temperature on the SPR response of the AAB/ ATP/Au immunosensor with different LDL concentrations were studied at 283 (Figure 3b), 298 (Figure 3a), and 308 K (Figure 3c), respectively. It was observed that the increase in temperature affected both resonance angle shift and rate of binding.…”

“…The SPR measurements were carried out on SPR instrument (Autolab, Eco Chemie, The Netherlands) having Kretschmann configuration. 16 In these studies, the SPR signal was recorded as a function of LDL concentration for the AAB/ATP/Au immunosensor. The QCM Instrumentation and Measurements.…”

“…In this context, Matharu et al have fabricated LDL sensor using specific LDL-heparin interaction by surface plasmon resonance (SPR) technique. 16 Jie et al have used apolipoprotein B-LDL interaction for the fabrication of CdS nanocrystal based electroluminescence biosensor for LDL detection. 17 Yan et al have used a highly specific antibody, antiapolipoprotein B, that acts as a LDL receptor and fabricated an electrochemical biosensor by adsorption of antiapolipoprotein B on gold nanoparticles-AgCl-polyaniline-modified glassy carbon electrode.…”

Lipid–Lipid Interactions in Aminated Reduced Graphene Oxide Interface for Biosensing Application

“…26 Matharu et al have reported an LDL immunosensor via specific LDLheparin interaction using a surface plasmon resonance (SPR) technique. 27 Jie et al have fabricated a cadmium sulfide nanocrystal based immunosensor for LDL detection using electroluminescence phenomenon. 28 Thus, the strategy based on the formation of an immunocomplex (via antigen−antibody interaction) for LDL detection may perhaps provide an efficient platform compared to those based on techniques like nuclear magnetic resonance spectroscopy, Friedewald equation, ultracentrifugation, electrophoresis, etc.…”

“…Interaction of antiapolipoprotein B 100 with sulfated glycosaminoglycans may result in cholesterol accumulation in the arterial walls that in turn perhaps leads to plaque formation resulting in the narrowing of the arteries making these less flexible causing various heart and vascular diseases . Matharu et al have reported an LDL immunosensor via specific LDL-heparin interaction using a surface plasmon resonance (SPR) technique . Jie et al have fabricated a cadmium sulfide nanocrystal based immunosensor for LDL detection using electroluminescence phenomenon .…”