Self-assembled nanoparticles based on glycol chitosan bearing 5β-cholanic acid for RGD peptide delivery

Park, Jae Hyung; Kwon, Seunglee; Nam, Ju Ock; Park, Rang Woon; Chung, Heesun; Seo, Sang Bong; Kim, In San; Kwon, Ick Chan; Jeong, Seo Young

doi:10.1016/j.jconrel.2003.12.020

Cited by 185 publications

(85 citation statements)

References 30 publications

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“…17 In addition, CNPs are able to carry diverse hydrophobic chemotherapeutic drugs and photodynamic chemicals by virtue of their hydrophobic core, and they are also effective in the delivery of hydrophilic agents. [34][35][36] Considering its wide availability and the fact that the intracellular delivery of CNPs is dramatically increased in the presence of collagen, this study implies a strong possibility that CNPs can be an effective drug delivery system to target fibrotic fibroblasts residing in the pathological microenvironment, such as IPF fibroblasts.…”

Section: Discussionmentioning

confidence: 97%

The effects of collagen-rich extracellular matrix on the intracellular delivery of glycol chitosan nanoparticles in human lung fibroblasts

Yhee¹,

Yoon²,

Kim³

et al. 2017

IJN

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Recent progress in nanomedicine has shown a strong possibility of targeted therapy for obstinate chronic lung diseases including idiopathic pulmonary fibrosis (IPF). IPF is a fatal lung disease characterized by persistent fibrotic fibroblasts in response to type I collagen-rich extracellular matrix. As a pathological microenvironment is important in understanding the biological behavior of nanoparticles, in vitro cellular uptake of glycol chitosan nanoparticles (CNPs) in human lung fibroblasts was comparatively studied in the presence or absence of type I collagen matrix. Primary human lung fibroblasts from non-IPF and IPF patients (n=6/group) showed significantly increased cellular uptake of CNPs (>33.6–78.1 times) when they were cultured on collagen matrix. To elucidate the underlying mechanism of enhanced cellular delivery of CNPs in lung fibroblasts on collagen, cells were pretreated with chlorpromazine, genistein, and amiloride to inhibit clathrin-mediated endocytosis, caveolae-mediated endocytosis, and macropinocytosis, respectively. Amiloride pretreatment remarkably reduced the cellular uptake of CNPs, suggesting that lung fibroblasts mainly utilize the macropinocytosis-dependent mechanism when interacted with collagen. In addition, the internalization of CNPs was predominantly suppressed by a phosphoinositide 3-kinase (PI3K) inhibitor in IPF fibroblasts, indicating that enhanced PI3K activity associated with late-stage macropinocytosis can be particularly important for the enhanced cellular delivery of CNPs in IPF fibroblasts. Our study strongly supports the concept that a pathological microenvironment which surrounds lung fibroblasts has a significant impact on the intracellular delivery of nanoparticles. Based on the property of enhanced intracellular delivery of CNPs when fibroblasts are made to interact with a collagen-rich matrix, we suggest that CNPs may have great potential as a drug-carrier system for targeting fibrotic lung fibroblasts.

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Section: Discussionmentioning

confidence: 97%

The effects of collagen-rich extracellular matrix on the intracellular delivery of glycol chitosan nanoparticles in human lung fibroblasts

Yhee¹,

Yoon²,

Kim³

et al. 2017

IJN

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“…The synthetic peptide bearing ArgGly-Asp (RGD) sequence is known to specifically bind to the αvβ3 integrin expressed on endothelial cells in the angiogenic blood vessels, which can potentially inhibit the tumor growth and proliferation. Following hydrophobic modifications, glycol chitosan is capable of forming self-aggregated nanotube and has been used as a carrier for the RGD peptide, labeled with fluoresein isothiocyanate (FITC-GRGDS) [27]. These nanotubes loaded with FITC-GRGDS might be useful for monitoring or destroying the angiogenic tissue/blood vessels surrounding the tumor tissue.…”

Section: Targeting Angiogenesis With Nanoparticlesmentioning

confidence: 99%

Nanotechnology-based Drug Delivery Systems

Suri¹,

Fenniri²,

Singh³

2011

Current Research in Pharmaceutical Technology

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Nanoparticles hold tremendous potential as an effective drug delivery system. In this review we discussed recent developments in nanotechnology for drug delivery. To overcome the problems of gene and drug delivery, nanotechnology has gained interest in recent years. Nanosystems with different compositions and biological properties have been extensively investigated for drug and gene delivery applications. To achieve efficient drug delivery it is important to understand the interactions of nanomaterials with the biological environment, targeting cell-surface receptors, drug release, multiple drug administration, stability of therapeutic agents and molecular mechanisms of cell signalling involved in pathobiology of the disease under consideration. Several anti-cancer drugs including paclitaxel, doxorubicin, 5-fluorouracil and dexamethasone have been successfully formulated using nanomaterials. Quantom dots, chitosan, Polylactic/glycolic acid (PLGA) and PLGA-based nanoparticles have also been used for in vitro RNAi delivery. Brain cancer is one of the most difficult malignancies to detect and treat mainly because of the difficulty in getting imaging and therapeutic agents past the blood-brain barrier and into the brain. Anti-cancer drugs such as loperamide and doxorubicin bound to nanomaterials have been shown to cross the intact blood-brain barrier and released at therapeutic concentrations in the brain. The use of nanomaterials including peptide-based nanotubes to target the vascular endothelial growth factor (VEGF) receptor and cell adhesion molecules like integrins, cadherins and selectins, is a new approach to control disease progression.

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“…Park and coworkers [123] reported the development of self-assembled hydrogel NPs capable of imbibing a peptide sequence that specifically binds to αvβ3 integrin. The authors observed that NPs made of hydrophobically modified CS could release the peptide in a sustained manner, and showed that they might be useful for monitoring or destroying angiogenic vessels.…”

Section: Integrinmentioning

confidence: 99%