Self-assembled nanoparticles of functional copolymers for photonic applications

Venditti, Iole; Fratoddi, Ilaria; Palazzesi, C.; Prosposito, P.; Casalboni, M.; Cametti, C.; Battocchio, Chiara; Polzonetti, Giovanni; Russo, Maria Vittoria

doi:10.1016/j.jcis.2010.04.061

Cited by 43 publications

(43 citation statements)

References 41 publications

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“…The peak at about 402 eV, observed for samples prepared at pH=2 and pH=5, is consistent with the protonated amine group, as expected for compounds containing quaternarized nitrogen, such as NH 4 X or (CH 3 ) 4 NX compounds. This peak was already observed for pristine PDMPAHCl nanoparticles, as reported elsewhere [43,44,45].…”

Section: Resultssupporting

confidence: 83%

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Bioconjugation of gold-polymer core–shell nanoparticles with bovine serum amine oxidase for biomedical applications

Venditti

Hassanein

Fratoddi

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Resultssupporting

confidence: 83%

“…6, panel b) confirm the occurrence of Cl − ions and a single signal is observed for all samples, with the 2p 3/2 main spin orbit component at about 198.0 eV [43].…”

Section: Resultssupporting

confidence: 59%

Bioconjugation of gold-polymer core–shell nanoparticles with bovine serum amine oxidase for biomedical applications

Venditti

Hassanein

Fratoddi

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…The functional groups content, i.e. COOH, NMe 2 or SH, which can be varied in the synthesis mixture is expected to affect the surface charge density, as reported for analogous co-polymers [31].…”

Section: Resultsmentioning

confidence: 87%

“…The co-polymeric nanoparticles P(MMA-co-AA), P(MMAco-DMPA), or P(MMA-co-AM) were synthesized by a modified emulsion technique with MMA/co-monomers molar ratios ranging from 10/1 to 2/1 and reaction times 1-20 h, according to the procedure already assessed for similar materials [31,32]. In a typical procedure, for example for P(MMA-co-AA), with MMA/AA molar ratio 5/1, 50 mL of deionized water, 1.00 mL (9.35 mmol) of MMA, and 0.13 mL (1.86 mmol) of co-monomer AA were mixed and degassed for 15 min under stirring in Argon atmosphere, at 80°C for 1 h. A water solution of KPS (50 mg in 5 mL) was added, and the reaction was refluxed under stirring in Ar atmosphere for 20 h. The polymerization was stopped by opening the flask and the light yellow emulsion was filtered, centrifuged at 2000 rpm and re-dispersed in deionized water several times, in order to remove all un-reacted chemicals.…”

Section: Synthesis and Characterization Of Functional Co-polymeric Namentioning

confidence: 99%

Nanostructured functional co-polymers bioconjugate integrin inhibitors

Laganà

Venditti

Fratoddi

et al. 2011

Journal of Colloid and Interface Science

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“…Zeta potential has been measured by using Malvern NanoZS90 apparatus with disposable folded capillary cuvettes, provided with electrodes. [47,48] [49,50]. Briefly, Au-3MPS and Au-DEA have been prepared with Au/thiol (Au/S) molar ratio 1/4 and 1/10 and the procedure is herein reported for Au/S 1/4 Au-3MPS sample.…”

Section: Instrumentsmentioning

confidence: 99%

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Bessar

Venditti

Benassi

et al. 2016

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tGold nanoparticles (AuNPs) represent an effective choice for topical drug delivery systems thanks to their small size, general non-toxicity, ease of functionalization and high surface to volume ratio. Even if systemic, methotrexate still plays an important role in psoriasis treatment: its topical use shows insufficient percutaneus penetration owing to limited passive diffusion, high molecular weight and dissociation at physiological pH. The aim of our study was to design a new drug delivery nanocarrier for Methotrexate and to improve its solubility, stability and biodistribution. AuNPs were on purpose prepared with a hydrophilic stabilizing layer, in order to improve the colloidal stability in water. Watersoluble gold nanoparticles functionalized by sodium 3-mercapto-1-propansulfonate (Au-3MPS) were prepared and loaded with methotrexate (MTX). The loading efficiency of MTX on Au-3MPS was assessed in the range 70-80%, with a fast release (80% in one hour). The release was studied up to 24 h reaching the value of 95%. The Au-3MPS@MTX conjugate was fully characterized by spectroscopic techniques (UV-vis, FTIR) and DLS. Preliminary toxicity tests in the presence of keratinocytes monolayers allowed to assess that the used Au-3MPS are not toxic. The conjugate was then topically used on C57BL/6 mouse normal skin in order to trace the absorption behavior. STEM images clearly revealed the distribution of gold nanoparticles inside the cells. In vitro studies showed that Methotrexate conjugated with Au-3MPS is much more efficient than Methotrexate alone. Moreover, DL50, based on MTT analysis, is 20 folds reduced at 48 h, by the presence of nanoparticles conjugation. UV-vis spectra for in vivo tracing of the conjugate on bare mouse skin after 24 h of application, show increased delivery of Methotrexate in the epidermis and dermis using Au-3MPS@MTX conjugate, compared to MTX alone. Moreover we observed absence of the Au-3MPS in the dermis and in the epidermis, suggesting that these layers of the skin do not retain the nanoparticles. Based on our data, we found that the novel Au-3MPS@MTX conjugate is an effective non-toxic carrier for the satisfactory percutaneous absorption of Methotrexate and could help in possible topical treatment of psoriasis.

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Self-assembled nanoparticles of functional copolymers for photonic applications

Cited by 43 publications

References 41 publications

Bioconjugation of gold-polymer core–shell nanoparticles with bovine serum amine oxidase for biomedical applications

Bioconjugation of gold-polymer core–shell nanoparticles with bovine serum amine oxidase for biomedical applications

Nanostructured functional co-polymers bioconjugate integrin inhibitors

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

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