Self-Assembled Organic Monolayers: Model Systems for Studying Adsorption of Proteins at Aurfaces

Prime, Kevin L.; Whitesides, George M.

doi:10.1126/science.252.5009.1164

Cited by 1,768 publications

(1,408 citation statements)

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“…This may be due to the greater hydrophobicity of these surfaces 31 , increasing the binding strength of hydrophobic interactions between BSA and the surface. It is also possible that the fluorocarbon waterproofing agent generates some small scale roughness and thus increases the area available for adsorption, although results using silane coupling agents were similar to these (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Nano-scale superhydrophobicity: suppression of protein adsorption and promotion of flow-induced detachment

et al. 2008

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Section: Resultsmentioning

confidence: 99%

Nano-scale superhydrophobicity: suppression of protein adsorption and promotion of flow-induced detachment

et al. 2008

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“…You can check this recommendation in the web site http://www.acdlabs.com/iupac/ nomenclature/93/r93_296.htm and http://www.acdlabs.com/iupac/ nomenclature/93/r93_302.htm), which can form a strong bond between a sulfanyl group and a gold surface. [63][64][65] However, as shown in recent studies, amine groups on non-hybridized nucleobases in ssDNA can also interact with gold surfaces effectively, becoming unavailable to hybridize with the target DNA molecules on the surface. Thus, extensive efforts to develop surface passivation techniques for the improvement of DNA density and orientation on a gold surface have been made.…”

Section: Construction Of Oligodna/peg Hybrid Surfaces and Their Perfomentioning

confidence: 99%

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Nagasaki

2011

Polym J

116

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Non-biofouling surfaces constitute one of the most important subjects in sensitively and selectively detecting biomolecular events. Poly(ethylene glycol) (PEG) chains tethered on substrate surfaces are well known to reduce non-biofouling characteristics. Protein adsorption onto a PEG-chain-tethered surface is strongly influenced by the density of the PEG chain and is almost completely suppressed by the successive treatment of longer PEG chains (5 kDa) followed by the treatment of PEG (2 kDa; mixed-PEG-chain-tethered surface) because of a significant increase in PEG chain density. To modify versatile substrate surface, PEG possessing pentaethylenehexamine at one end (N6-PEG) was prepared via a reductive amination reaction of aldehyde-ended PEG with pentaethylenehexamine. Using N6-PEG, antibody/PEG co-immobilization was conducted on a substrate possessing active ester groups. After the antibody was immobilized on the surface, PEG tethered chains were constructed surrounding the immobilized antibody. It is interesting to note that the PEG-chain-tethered functions not only as a non-fouling agent but also improves immune response. The hybrid surface was also applied to oligo DNA immobilization. The oligo DNA/PEG hybrid surface improved hybridization, retaining its non-fouling ability. Densely packed PEG tethered chains surrounding antibodies and/or oligo DNA improved their orientation on the surface. Thus, this material is promising as a high-performance biointerface for versatile applications. Keywords: antibody; biosensing; DNA; end-functionalized poly(ethylene glycol); enzyme-linked immunosorbent assay; hybrid biointerface; soft interface INTRODUCTION Specific biorecognition on substrate surfaces has long been utilized in many applications such as biosensing, 1 immunodiagnostics, 2 enzyme immunoassay, 3 western blotting 4 and protein microarrays. 5 Important factors for the improvement in specific biorecognition on surfaces are the suppression of nonspecific biofouling and the suitable orientation of immobilized biomolecules. The former decreases nonspecific noise and the background level, and the latter increases the specific recognition level. To improve the non-biofouling character of surfaces, numerous efforts have been undertaken. In general, natural polymers such as albumin, casein and dextran have been used for blocking on substrate surfaces. 6 These treatments work well and suppress protein biofouling extensively. If extremely high performance is required, however, these blocking treatments are not enough. In addition, natural products, especially those derived from animals, may have undesired contents such as bacteria and viruses, which may affect the user. 7 Under these circumstances, synthetic polymers have been recently suggested as suitable surface blocking agents. Numerous types of water-soluble polymers, such as poly(acrylamide), poly(vinyl alcohol), poly(vinyl pyrrolidone) and poly(ethylene glycol) (PEG), have been investigated so far. Recently, new types of synthetic polymers such as poly(methoxy ...

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“…However, there is still lack in understanding of the detailed adsorption phenomenon. [2][3][4][5][6][7] A self-assembled monolayer ͑SAM͒ can be defined as a functional assembly of molecules. These molecules typically have a head group that interacts with or binds to a surface.…”

Section: Introductionmentioning

confidence: 99%

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

et al. 2009

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Tethered bilayer lipid membranes are promising biomimetic architectures. Their formation has been investigated using four different surface sensitive techniques, including optical, acoustic, and electrical methods. The lipid bilayers are built in a two-step procedure; the proximal layer is formed by self-assembly and is then completed to a bilayer by fusion with small vesicles. The different technical approaches revealed specific aspects of the layer formation processes, namely, first a fast adsorption process followed by a longer rearrangement period. Similar phenomena have been observed for the vesicle fusion process. The results allow for a more controlled assembly protocol for the preparation of highly insulating lipid membranes.

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Self-Assembled Organic Monolayers: Model Systems for Studying Adsorption of Proteins at Aurfaces

Cited by 1,768 publications

References 39 publications

Nano-scale superhydrophobicity: suppression of protein adsorption and promotion of flow-induced detachment

Nano-scale superhydrophobicity: suppression of protein adsorption and promotion of flow-induced detachment

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

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