Self-assembled peptide nanomaterials for biomedical applications: promises and pitfalls

Sun, Linlin; Zheng, Chunli; Webster, Thomas J.

doi:10.2147/ijn.s117501

Cited by 153 publications

(76 citation statements)

References 106 publications

(146 reference statements)

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“…Self‐assembling oligopeptides have been identified as promising candidates in the development of the next generation of biomaterials . Since they are based on a limited number of building blocks, these systems reduce the complexity of intramolecular and intermolecular interactions, providing interesting models for the investigation of biological phenomena closely related to protein aggregation .…”

Section: Introductionmentioning

confidence: 99%

β‐sheet assembly in amyloidogenic glutamic acid nanostructures: Insights from X‐ray scattering and infrared nanospectroscopy

Mello

Hamley

Miranda

et al. 2019

Journal of Peptide Science

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Glutamic acid–rich peptides are crucial to a variety of biological processes, including glutamatergic neurotransmission and immunological defense. Glutamic acid sequences often exhibit unusual organization into β2‐type sheets, where bifurcated H bonds formed between glutamic acid side chains and NH in amide bonds on adjacent β‐strands play a paramount role for stabilizing the molecular assembly. Herein, we investigate the self‐assembly and supramolecular structure of simplified models consisting of alternating glutamic acid/phenylalanine residues. Small‐angle X‐ray scattering and atomic force microscopy show that the aggregation pathway is characterized by the formation of small oligomers, followed by coalescence into nanofibrils and nanotapes. Amyloidogenic features are further demonstrated through fiber X‐ray diffraction, which reveal molecular packing according to cross‐β patterns, where β‐strands appear perpendicularly oriented to the long axis of nanofibrils and nanotapes. Nanoscale infrared spectroscopy from individual nanoparticles on dried samples shows a remarkable decrease of β2‐sheet content, accompanied by growth of standard β‐sheet fractions, indicating a β2‐to‐β1 transition as a consequence of the release of solvent from the interstices of peptide assemblies. Our findings highlight the key role played by water molecules in mediating H‐bond formation in β2‐sheets commonly found in amyloidogenic glutamic acid–rich aggregates.

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Section: Introductionmentioning

confidence: 99%

β‐sheet assembly in amyloidogenic glutamic acid nanostructures: Insights from X‐ray scattering and infrared nanospectroscopy

Mello

Hamley

Miranda

et al. 2019

Journal of Peptide Science

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“…[16][17][18] For the past several decades, biomedical applications including tissue engineering, drug delivery, bioimaging, and nanodiagnostics have been promoted by the nanotechnology significantly. [19][20][21][22] Among these applications, nanodiagnostics have drawn more and more attention for the infectious diseases because of their unique characteristics in early detection, high sensitivity, and the potential for POCT.…”

Section: Introductionmentioning

confidence: 99%

Application of nanodiagnostics in point-of-care tests for infectious diseases

Wang¹,

Yu²,

Kong³

et al. 2017

IJN

103

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Although tremendous efforts have been put into the treatment of infectious diseases to prevent epidemics and mortality, it is still one of the major health care issues that have a profound impact on humankind. Therefore, the development of specific, sensitive, accurate, rapid, low-cost, and easy-to-use diagnostic tools is still in urgent demand. Nanodiagnostics, defined as the application of nanotechnology to medical diagnostics, can offer many unique opportunities for more successful and efficient diagnosis and treatment for infectious diseases. In this review, we provide an overview of the nanodiagnostics for infectious diseases from nanoparticle-based, nanodevice-based, and point-of-care test (POCT) platforms. Most importantly, emphasis focused on the recent trends in the nanotechnology-based POCT system. The current state-of-the-art and most promising point-of-care nanodiagnostic technologies, including miniaturized diagnostic magnetic resonance platform, magnetic barcode assay system, cell phone-based polarized light microscopy platform, cell phone-based dongle platform, and paper-based POCT platform, for infectious diseases were fully examined. The limitations, challenges, and future trends of the nanodiagnostics in POCTs for infectious diseases are also discussed.

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“…3 To improve these shortcomings, a large number of nanoscale drug carriers, such as polymeric nanomicelles, inorganic nanoparticles and liposomes and so on, have been developed to achieve the tumor-targeted delivery of chemotherapeutic drugs. [4][5][6] Among them, selenium nanoparticles (SeNPs) have received an increasing attention as drug carriers owing to their biocompatibility, low toxicity, simple synthesis process, in vivo degradability and excellent chemopreventive effects. 7 Selenium is a mineral trace element of fundamental importance to humans and animals.…”

Section: Introductionmentioning

confidence: 99%

Novel functionalized nanoparticles for tumor-targeting co-delivery of doxorubicin and siRNA to enhance cancer therapy

Xia

Wang

et al. 2017

IJN

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Human homeobox protein (Nanog) is highly expressed in most cancer cells and has gradually emerged as an excellent target in cancer therapy, owing to its regulation of cancer cell proliferation, metastasis and apoptosis. In this study, we prepared tumor-targeting functionalized selenium nanoparticles (RGDfC-SeNPs) to load chemotherapeutic doxorubicin (DOX) and Nanog siRNA. Herein, RGDfC peptide was used as a tumor-targeting moiety which could specifically bind to α v β 3 integrins overexpressed on various cancer cells. The sizes of RGDfC-SeNPs@DOX nanoparticles (~12 nm) were confirmed by both dynamic light scattering and transmission electron microscopy. The chemical structure of RGDfC-SeNPs@DOX was characterized via Fourier-transform infrared spectroscopy. The RGDfC-SeNPs@DOX was compacted with siRNA (anti-Nanog) by electrostatic interaction to fabricate the RGDfC-SeNPs@DOX/siRNA complex. The RGDfC-SeNPs@DOX/siRNA complex nanoparticles could efficiently enter into HepG2 cells via clathrin-associated endocytosis, and showed high gene transfection efficiency that resulted in enhanced gene silencing. The in vivo biodistribution experiment indicated that RGDfC-SeNPs@DOX/siRNA nanoparticles were capable of specifically accumulating in the tumor site. Furthermore, treatment with RGDfC-SeNPs@DOX/siRNA resulted in a more significant anticancer activity than the free DOX, RGDfC-SeNPs@DOX or RGDfC-SeNPs/siRNA in vitro and in vivo. In summary, this study shows a novel type of DOX and siRNA co-delivery system, thereby providing an alternative route for cancer treatment.

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Self-assembled peptide nanomaterials for biomedical applications: promises and pitfalls

Cited by 153 publications

References 106 publications

β‐sheet assembly in amyloidogenic glutamic acid nanostructures: Insights from X‐ray scattering and infrared nanospectroscopy

β‐sheet assembly in amyloidogenic glutamic acid nanostructures: Insights from X‐ray scattering and infrared nanospectroscopy

Application of nanodiagnostics in point-of-care tests for infectious diseases

Novel functionalized nanoparticles for tumor-targeting co-delivery of doxorubicin and siRNA to enhance cancer therapy

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