Self-assembled selenium nanoparticles and their application in the rapid diagnostic detection of small cell lung cancer biomarkers

Abstract

By coupling molecular imprinting, chitosan biosorption and TiO photocatalysis, selenium nanoparticles (Se NPs) were self-assembled in a controlled manner on the molecular imprinting sites of zeolite-chitosan-TiO microspheres. Se NPs with different sizes and areal densities were individually synthesized by controlling the rapid adsorption of molecular-imprinted nanocomposites and photocatalytic reaction of TiO nanoparticles. In order to improve the sensitivity and specificity of rapid diagnostic detection, Se NPs were self-assembled again into high-order and spherically stable structures with an average size of 80 nm by well-defined monomer units, after separation from zeolite-chitosan-TiO microspheres with a stabilizer of 0.3% (v/v) bovine serum albumin. Due to their biological activity, spherical-shaped Se NPs were used for dot-blot immunoassays with multiple native antigens for rapid serodiagnosis of human lung cancer. The sensitivity of the dot immunoassays for detecting progastrin-releasing peptide (ProGRP) was 75 pg mL. The detection time of colloidal Se dot immunoassays for ProGRP was only 5 min. No positive results were observed with other commonly potential interfering substances, including carcinoembryonic antigen, α-fetoprotein antigen and BSA. The research presents a simple and green method for the reuse of SeO and the controlled synthesis of Se NPs for biological and medical applications by bioaffinity adsorption and photoreduction.

Citric juice-mediated synthesis of tellurium nanoparticles with antimicrobial and anticancer properties

“…On the other hand, chalcogens, such as selenium (Se) and tellurium (Te) are gaining special interest in nanomedicine for biomedical applications 17,18,19 . Previous research on the antibacterial properties of tellurium started as early as 1932, and the antimicrobial activity of the metalloid was even reported before the wide-spread use of antibiotics 20 .…”

Selenium Nanoparticles for Biomedical Applications: From Development and Characterization to Therapeutics

“…This serodiagnosis technique took 5 min and its sensitivity was 75 pg mL −1 . [ 223 ] Also, Korany et al. studied the use of SeNPs radiolabelled with 99m Tc, producing 99m Tc‐GSH/SeNPs with liver cancer theragnosis potential in male Swiss albino mice.…”

Selenium Species: Current Status and Potentials in Cancer Prevention and Therapy

“…Aside from direct therapeutic effects, chemically modified SeNPs can function as vehicles that endow the carried objects with favorable properties like tumor targeting [150,157], high efficacy [137,144], and low toxicity [139]. Notably, chemosynthetic SeNPs were also examined as diagnostic agents [145,147,149], imaging agents [146,153], and radiosensitizers [130,158]. Overall, chemosynthesis is the most common method used to obtain and modify SeNPs, because the process is easy to implement and control.…”