Self-assembled short peptides: Recent advances and strategies for potential pharmaceutical applications

Shen, Yang; Wang, Mingge; Wang, Tianye; Sun, Mengchi; Huang, Hanwei; Shi, Xianbao; Duan, Shijie; Wu, Ying; Zhu, Jiaming; Liu, Funan

doi:10.1016/j.mtbio.2023.100644

Cited by 25 publications

(6 citation statements)

References 212 publications

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“…This is what we have demonstrated in the present work. Short peptides rarely exhibit toxic side effects [ 42 , 43 , 44 ]. It can be suggested with a high probability that the active peptide concentration chosen herein (100 nM) is safe due to the endogenous nature of the investigated short peptide.…”

Section: Discussionmentioning

confidence: 99%

Analgesic Effect of the Lysine-Containing Short Peptide Is Due to Modulation of the NaV1.8 Channel Activation Gating System

Kalinina,

Rogachevskii,

Samosvat

et al. 2023

Life

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The present work continues our recent series of articles that aim to elucidate the ligand–receptor binding mechanism of short cationic peptides to the NaV1.8 channel in the nociceptive neuron. The applied methodological approach has involved several methods: the patch-clamp experimental evaluation of the effective charge of the NaV1.8 channel activation gating system, the organotypic tissue culture method, the formalin test, and theoretical conformational analysis. The lysine-containing short peptide Ac-KEKK-NH2 has been shown to effectively modulate the NaV1.8 channel activation gating system. As demonstrated by the organotypic tissue culture method, the studied short peptide does not trigger the downstream signaling cascades controlling neurite outgrowth and should not be expected to evoke adverse side effects. Conformational analysis of the Ac-KEKK-NH2 molecule has revealed that the distances between the positively charged amino groups of the lysine side chains are equal to 11–12 Å. According to the previously suggested mechanism of ligand–receptor binding of short peptides to the NaV1.8 channel molecule, Ac-KEKK-NH2 should exhibit an analgesic effect, which has been confirmed by the formalin test. The data obtained unequivocally indicate that the studied lysine-containing short peptide is a promising candidate for the role of a novel analgesic medicinal substance.

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Section: Discussionmentioning

confidence: 99%

Analgesic Effect of the Lysine-Containing Short Peptide Is Due to Modulation of the NaV1.8 Channel Activation Gating System

Kalinina,

Rogachevskii,

Samosvat

et al. 2023

Life

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“…Amphillic amino acids such as cysteine can facilitate the formation of the micelle-like vesicles. 115 5.1. Design of Self-Assembled Peptides.…”

Section: Self-assembled Peptidesmentioning

confidence: 99%

“…Positively and negatively charged amino acids, such as lysine, arginine, glutamic acid, and aspartic acid, can influence the electrostatic interactions within the self-assembled peptides. Amphillic amino acids such as cysteine can facilitate the formation of the micelle-like vesicles …”

Section: Self-assembled Peptidesmentioning

confidence: 99%

Advances in Peptide-Decorated Targeted Drug Delivery: Exploring Therapeutic Potential and Nanocarrier Strategies

Buddhiraju,

Yadav,

Dey

et al. 2023

ACS Appl. Bio Mater.

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Peptides are ideal biologicals for targeted drug delivery and have also been increasingly employed as theranostic tools in treating various diseases, including cancer, with minimal or no side effects. Owing to their receptor-specificity, peptidemediated drug delivery aids in targeted drug delivery with better pharmacological biodistribution. Nanostructured self-assembled peptides and peptide−drug conjugates demonstrate enhanced stability and performance and captivating biological effects in comparison with conventional peptides. Moreover, they serve as valuable tools for establishing interfaces between drug carriers and biological systems, enabling the traversal of multiple biological barriers encountered by peptide−drug conjugates on their journeys to their intended targets. Peptide-based drugs play a pivotal role in the field of medicine and hold great promise for addressing a wide range of complex diseases such as cancer and autoimmune disorders. Nanotechnology has revolutionized the fields of medicine, biomedical engineering, biotechnology, and engineering sciences over the past two decades. With the help of nanotechnology, better delivery of peptides to the target site could be achieved by exploiting the small size, increased surface area, and passive targeting ability of the nanocarrier. Furthermore, nanocarriers also ensure safe delivery of the peptide moieties to the target site, protecting them from degradation. Nanobased peptide delivery systems would be of significant importance in the near future for the successful targeted and efficient delivery of peptides. This review focuses on peptide−drug conjugates and nanoparticle-mediated self-assembled peptide delivery systems in cancer therapeutics.

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“…Alkyl chain modification of peptides has received much attention in recent years (Evans et al 2020 ; Liu et al 2019 ; Yang et al 2023 ), since alkyl chain introduction renders peptides hydrophobic, which can induce peptide self-assembly and lead to the improvement of peptide drug stability (Gao et al 2023 ; Ding et al 2023 ; Yang et al 2023 ). Our previous study showed that self-assembled micelles formed by N-terminal myristoylated MVIIA at high concentrations can prolong the duration of analgesic effect, improve serum stability, and significantly reduce or even eliminate side effects (Ding et al 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Reductive amination of ω-conotoxin MVIIA: synthesis, determination of modification sites, and self-assembly

Ding,

Wang,

Zhang

et al. 2024

Amino Acids

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Peptide drugs have disadvantages such as low stability, short half-life and side effects, which limit their widespread use in clinical practice. Therefore, peptide drugs can be modified to improve these disadvantages. Numerous studies have shown that alkyl-modified peptide drugs can self-assemble to prolong the duration of efficacy and/or reduce side effects. However, the commonly used solid-phase synthesis method for alkyl-modified peptides is time-consuming. To overcome this, a simple reductive amination reaction was employed, which can directly graft the alkyl chain to the peptide sequence and effectively avoid stepwise synthesis from C- to N-terminal with amino acids. In this study, ω-conotoxin MVIIA was used as the peptide drug, while myristic aldehyde was used as the alkylating agent. To obtain the maximum productivity of modified peptides, the molar ratio of peptide MVIIA to myristic aldehyde in the reductive amination reaction was optimized. Furthermore, the peptide modification sites in this reaction were confirmed by secondary mass spectrometry analysis. Besides, alkyl-modified peptide MVIIA was able to form micelles by self-assembly and improved stability in serum, which was related to our previous work where myristoylated peptide MVIIA micelles can improve the drug stability. Finally, this study was intended to provide a methodological basis for modifying the alkyl chain of peptide drugs.

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Self-assembled short peptides: Recent advances and strategies for potential pharmaceutical applications

Cited by 25 publications

References 212 publications

Analgesic Effect of the Lysine-Containing Short Peptide Is Due to Modulation of the NaV1.8 Channel Activation Gating System

Analgesic Effect of the Lysine-Containing Short Peptide Is Due to Modulation of the NaV1.8 Channel Activation Gating System

Advances in Peptide-Decorated Targeted Drug Delivery: Exploring Therapeutic Potential and Nanocarrier Strategies

Reductive amination of ω-conotoxin MVIIA: synthesis, determination of modification sites, and self-assembly

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