Self-assembling peptide hydrogels immobilized on silicon surfaces

Franchi, Stefano; Battocchio, Chiara; Galluzzi, Martina; Navisse, Emanuele; Zamuner, Annj; Dettin, Monica; Iucci, Giovanna

doi:10.1016/j.msec.2016.06.060

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…1e. The position of the single component at the binding energy of 400.3 eV corresponded to expected value for peptidic nitrogen atoms [23,24]. Both the C 1s and N 1s spectra confirm the binding of HELP coating on both borosilicate and Fig.…”

Section: Helps Primary Structures and Coatingssupporting

confidence: 63%

Epidermal Growth Factor – based adhesion substrates elicit myoblast scattering, proliferation, differentiation and promote satellite cell myogenic activation

D’Andrea

Sciancalepore

Veltruská

et al. 2019

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Section: Helps Primary Structures and Coatingssupporting

confidence: 63%

Epidermal Growth Factor – based adhesion substrates elicit myoblast scattering, proliferation, differentiation and promote satellite cell myogenic activation

D’Andrea

Sciancalepore

Veltruská

et al. 2019

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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“…Here, we used the N K-edge of EP1-EP5 samples deposited on CVD graphene to probe the amide π orbital functionality along the peptide backbone, aiming to obtain an overall molecular orientation on the surface. [50][51][52] It is worth mentioning that the amino acid residues from peptides interact non-covalently with CNT and graphene in the same qualitative way, mainly through π-π stacking interactions. [53] The difference emerges in this interaction's magnitude, reportedly weaker for CNT than graphene.…”

Section: Resultsmentioning

confidence: 99%

Rational Design of Peptide Biorecognition Elements on Carbon Nanotubes for Sensing Volatile Organic Compounds

Sim

Kuang

Sant'Anna

et al. 2022

Adv Materials Inter

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compounds. Exhaled breath is one of the most accessible and zero-invasive samples to monitor human health and performance. For example, recent studies have demonstrated that analyzing the exhaled breath is practical for fast-diagnosing of COVID-19. [1,2] Volatile organic compounds (VOCs) [3][4][5][6] in the exhaled breath serve as performance-related biomarkers providing rich information on human physiological/physical statuses [7][8][9][10][11] such as psychological stress and fatigue level. A miniaturized sensor is key to building a wearable sensing suite with the capability to monitor human breath. Conventional VOC detection tools are either bulky or non-selective. For example, gas chromatography-mass spectrometry (GC-MS) is accepted as a gold standard for measuring breath VOCs. [12][13][14] However, GC-MS is bulky due to its heating and vacuum systems and requires a long operation time due to the VOC sampling and separation process. Photo-ionized detectors (PIDs) are another commercial off-the-shelf, portable VOC sensors commonly applied to gas sensing. [15,16] These sensors (VOC-TRAQ, MOCON, Inc., Minneapolis, MN, USA) range their dimensions Carbon nanotube (CNT) chemiresistors have emerged as miniaturized platforms for wearable volatile organic compound (VOC) sensors. As a promising biorecognition element (BRE), a short peptide can functionalize CNT to be sensitive and selective to target VOCs. However, unveiling the VOCoptimized peptide-CNT pair for gas-phase sensing remains unclear. Here, a novel multimodal molecular toolset for designing, building, and probing suitable BRE-CNT sensors using machine learning, molecular dynamics, and near-edge X-ray absorption fine structure spectroscopy is presented. This computational and experimental suite predicts the peptide conformation on the CNT surface and probes how the peptide-CNT interfaces affect the VOC sensing. Then, peptide-functionalized CNT chemiresistors are tested against various VOCs to confirm the efficacy of the toolkit. The results show that the vertically oriented peptide on the CNT surface hinders VOC access to the peptide-CNT interface, resulting in a significantly lower sensor signal than the CNT chemiresistor with the horizontally oriented peptide. The interactive computational and experimental results strongly indicate that a peptide conformation plays an important role in VOC sensing sensitivity.

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“…In addition, our carbon K-edge spectra were further calibrated using the resonance at 285.50 eV assigned to the C1s π* ring transition. The spectra were then normalized subtracting a straight line that fits the part of the spectrum below the edge and assessing to 1 the value at 320.00 eV and 425.00 eV for carbon and nitrogen, respectively [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore a possible strategy to promote osseo-integration and enhance the biological acceptance of the implants is the biofunctionalization of the Ti25Nb10Zr surface with bioactive molecules that can be grafted on the surface in order to establish a molecular dialogue with host cells [ 47 ]. Among other bioactive molecules, self-assembling peptides (SAPs) are extremely promising candidates, since thanks to their on-purpose designed sequence they are able to self-assemble in a beta-sheet secondary structure [ 48 , 49 ]. They can then aggregate in the presence of saline creating hydrogels that can be used either as drug delivery vehicles, in the case of factors to release with a precise kinetic, or can be decorated with adhesive sequences or proteins, appropriately conjugated with a self-assembling sequence, allowing the functionalization of the scaffold with adhesive signals in a 3D structure by simple co-aggregation.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Materials Based on Self-Assembling Peptides on Ti25Nb10Zr Alloy: Molecular Structure and Organization Investigated by Synchrotron Radiation Induced Techniques

et al. 2018

Self Cite

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In this work, we applied advanced Synchrotron Radiation (SR) induced techniques to the study of the chemisorption of the Self Assembling Peptide EAbuK16, i.e., H-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-NH2 that is able to spontaneously aggregate in anti-parallel β-sheet conformation, onto annealed Ti25Nb10Zr alloy surfaces. This synthetic amphiphilic oligopeptide is a good candidate to mimic extracellular matrix for bone prosthesis, since its β-sheets stack onto each other in a multilayer oriented nanostructure with internal pores of 5–200 nm size. To prepare the biomimetic material, Ti25Nb10Zr discs were treated with aqueous solutions of EAbuK16 at different pH values. Here we present the results achieved by performing SR-induced X-ray Photoelectron Spectroscopy (SR-XPS), angle-dependent Near Edge X-ray Absorption Fine Structure (NEXAFS) spectroscopy, FESEM and AFM imaging on Ti25Nb10Zr discs after incubation with self-assembling peptide solution at five different pH values, selected deliberately to investigate the best conditions for peptide immobilization.

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Self-assembling peptide hydrogels immobilized on silicon surfaces

Cited by 4 publications

References 23 publications

Epidermal Growth Factor – based adhesion substrates elicit myoblast scattering, proliferation, differentiation and promote satellite cell myogenic activation

Epidermal Growth Factor – based adhesion substrates elicit myoblast scattering, proliferation, differentiation and promote satellite cell myogenic activation

Rational Design of Peptide Biorecognition Elements on Carbon Nanotubes for Sensing Volatile Organic Compounds

Biocompatible Materials Based on Self-Assembling Peptides on Ti25Nb10Zr Alloy: Molecular Structure and Organization Investigated by Synchrotron Radiation Induced Techniques

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