Self-assembling Pluronic®-modified polycations in gene delivery

Dual-degradable disulfide-containing PEI–Pluronic/DNA polyplexes: transfection efficiency and balancing protection and DNA release

“…[23][24][25] Hydrophilic Pluronic polymers (poly[ethylene oxide]-b-poly[propylene oxide]-b-poly[ethylene oxide]) have been extensively used to enhance the efficiency of transfection of some cationic polymers and viral vectors in vitro and in vivo due to their excellent biocompatibility and environmental sensitivity. 26,27 The biocompatibility of the cationic polymer might be improved by grafting with Pluronic copolymers in a similar fashion to that observed with polyethylene glycol (PEG)-ylation. 28 Guo et al 29 reported that Pluronic-grafted PEI displayed higher transfection activity with lower cytotoxicity than 25 kDa PEI, owing to the ability of the Pluronic copolymer to adhere to the cell membrane.…”

Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer

“…The key attribute for the biological activity of Pluronics is their ability to incorporate into membranes, translocate into cells, and subsequently, affect various cellular functions, such as mitochondrial respiration, ATP synthesis, the activity of drug efflux transporters (Batrakova et al, 2008;Demina et al, 2005). To increase the delivery and transfection efficiency of nucleic acid medicine, Pluronics were used to modify traditional cationic polymers Bromberg et al, 2006). The cationic polymers modified with P123 include PEI and poly (2-N-(dimethylaminoethyl) methacrylate) (pDMAEMA) and the Pluronics used include P123, P85, F38, F127 and L92 Nguyen et al, 2000;Bromberg et al, 2005;Belenkov et al, 2004).…”

Interfacial properties of Pluronics and the interactions between Pluronics and cholesterol/DPPC mixed monolayers

“…Their amphiphilic characteristics as well as other physicochemical properties are critically dependent on the molecular architecture which could be finely tuned to meet specific application needs [1]. Pluronics have been applied in drug delivery system, in gene delivery, as biological adjuvants and so on [1][2][3].…”