“…To date, tremendous efforts have been devoted to developing new BOD detection methods to reduce assay time and simplify procedures to meet the requirements of end users [4,7,[9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26], such as (i) biosensors based on biolumines-cent bacteria, (ii) biosensors with redox mediators, (iii) microbial fuel cell (MFC) biosensor, (iv) biosensors with entrapped microorganisms, and (v) biosensors based on the bioreactor/chemist at technology [2,5]. Among them, because of the complexity and low precision of the bioluminescence reaction, the two former technologies show the least potential in practical applications [5,20,22,23,27,28]. Currently, the most widely adopted approach is the type of biosensor method based on the respiration rate [5,26].…”