Self‐Contained Handheld Magnetic Platform for Point of Care Cytometry in Biological Samples

Abstract

Magnetic levitation is a powerful tool capable of distinguishing micrometerscale particles based on their densities. When a particle is suspended in a paramagnetic medium and placed in a magnetic field formed by two permanent magnets with like poles facing each other, the particle will experience two forces-a magnetic force and a buoyant force. These forces cause a particle, such as a cell, which does not necessarily have magnetic properties, to levitate at a height inversely proportional to its density. Magnetic levitation has been shown to be useful for a range of applications, including disease diagnostics, material characterization, and quality control. Here, a portable, self-contained device fully independent from a dedicated microscope or smartphone is developed to levitate particles of interest, image them using an embedded low-cost optical system and a camera module, and process the captured images in order to estimate the densities of the particles. The device is user-friendly and inexpensive, offering a great potential for rapid, on-site sample analysis such as white blood cell cytometry.

Magnetische Levitation in Chemie, Materialwissenschaft und Biochemie

“…Insbesondere die Demirci‐Gruppe hat innovative Beiträge zur Trennung, Analyse und Selbstorganisation biologischer Zellen (insbesondere Einzelzellenanalyse) geleistet . Ghiran, Tasoglu und Kollegen haben eine Reihe neuer Techniken (insbesondere beim kombinierten Einsatz von MagLev und Smartphones) und Anwendungen in der medizinischen Analyse (Zellen und Assays) entwickelt . Die Yildiz‐Gruppe hat eine Methode zur Durchführung gerüstfreier Zellkulturen entwickelt .…”

“…Demirci, Ghiran, Tasoglu, Arslan‐Yildiz und Mitarbeiter haben MagLev um dichtebasierte Analysen und Trennungen einzelner Zellen erweitert, einschließlich der Entwicklung eines diagnostischen Prototyps zur Diagnose von Sichelzellkrankheiten (Abbildung F) . Tabelle fasst die Anwendungen von MagLev zur Analyse, Trennung, und Manipulation von Zellen (von Bakterien und Hefezellen bis hin zu Säugetierzellen und ganzen Organismen) zusammen.…”

3D printing for drug manufacturing: A perspective on the future of pharmaceuticals

“…This technology has been applied in numerous fields, representing the large variety of possible applications, including the consumer goods industry [1] , aerospace research [2,3] , regenerative medicine [4][5][6][7][8][9][10][11][12] , medical device development [13][14][15][16][17][18][19] and the automotive industry [20] . An emerging application of 3D printing is for drug manufacturing [21,22] .…”

Evolving Magnetically Levitated Plasma Proteins Detects Opioid Use Disorder as a Model Disease

“…It was shown that specific diseases show characteristic changes in density for particular types of cells. Tasoglu and co‐workers reported a portable and self‐contained device offering a great potential for rapid and on site sample analysis such as white blood cell cytometry . Their point‐of‐care Maglev device was able to perform cell cytometry of a particular population of cells (i.e., white blood cells) from a mixed population in human blood.…”

“…Tasoglu and co-workers reported a portable and self-contained device offering a great potential for rapid and on site sample analysis such as white blood cell cytometry. [4] Their point-of-care Maglev device was able to perform cell cytometry of a particular population of cells (i.e., white blood cells) from a mixed population in human blood. They have also combined Maglev technique with fluorescence imaging to provide specific clinical assays.…”