2016
DOI: 10.1016/j.eurpolymj.2016.06.010
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Self-reporting hydrogels rapidly differentiate among enterohemorrhagic Escherichia coli (EHEC) and non-virulent Escherichia coli (K12)

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Cited by 16 publications
(16 citation statements)
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“…With increasing reaction time, the color became more intense. Qualitatively similar observation in E. coli suspension indicates the presence of the enzyme also in the LB medium ( Figure 4 ), in agreement with previously published work [ 29 ]. The quantitative analysis of the photographs and the enzymatic reaction kinetics is key for an application of related sensors in the future.…”
Section: Discussionsupporting
confidence: 92%
See 1 more Smart Citation
“…With increasing reaction time, the color became more intense. Qualitatively similar observation in E. coli suspension indicates the presence of the enzyme also in the LB medium ( Figure 4 ), in agreement with previously published work [ 29 ]. The quantitative analysis of the photographs and the enzymatic reaction kinetics is key for an application of related sensors in the future.…”
Section: Discussionsupporting
confidence: 92%
“…Hence a promising strategy is the combination of feasible bacteria detection approaches with a paper-based carrier system that was investigated here for the E. coli marker enzyme β-glucuronidase (ß-Gus) and the non-pathogenic E. coli Mach1 lab strain as a model organism ( Figure 1 ) with smartphone-based detection, readout and quantitative data analysis. Previously, chitosan hydrogels equipped with different fluorogenic or chromogenic substrates were shown to rapidly, selectively and sensitively detect and differentiate among different strains of bacteria with LOD for the enzymes in several cases <1 nM after 1 h observation time [ 16 , 29 ]. As we report here, chitosan-based autonomously self-reporting hydrogels were successfully adapted to this paper carrier-based format to detect and quantify concentrations of bacterial enzymes.…”
Section: Introductionmentioning
confidence: 99%
“…The details are shown in Table S1 . Additionally, the values for the LOD for the detection of the enzymes at an observation time of 60 min are much lower than the enzyme concentrations in overnight cultured bacteria suspension, which are about 7–85 μM of α-glucosidase in S. aureus [ 49 ], 60 nM of β-glucuronidase in E. coli [ 61 ], and about 7 nM of β-galactosidase in EHEC [ 67 ].
Fig.
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Section: Resultsmentioning
confidence: 99%
“…certain EHEC and non EHEC E . coli strains, which was previously shown with an autonomously reporting chitosan hydrogel film sensor [ 67 ].…”
Section: Introductionmentioning
confidence: 85%
“…In addition, it was shown that this approach can be expanded to application in selective assays, e.g. to differentiate Staphylococcus aureus from Pseudomonas aeruginosa (with clear medical relevance) or to differentiate the S‐1 level E. coli ( K12 ) from the S‐3 level pathogenic E. coli EHEC (with implications for food safety) …”
Section: Introductionmentioning
confidence: 99%