Semen analysis in human immunodeficiency virus infection

Crittenden, Jenny A.; Handelsman, David J.; Stewart, Graeme J.

doi:10.1016/0020-7292(93)90857-s

Cited by 48 publications

(61 citation statements)

References 15 publications

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“…AIDS patients, which could be attributed to the changes in fertility parameters [7,16], an observation confirmed in the present study.…”

supporting

confidence: 89%

“…In contrast, untreated men in the advanced stage of AIDS have low sperm count and increased percentage of abnormal sperm forms [10]. Fewer motile sperm and more round cells were present in HIV-positive men regardless of stage of infection [7].…”

Section: E Umapathy Et Almentioning

confidence: 99%

“…HIV seropositivity is associated with leucocytospermia and impaired sperm motility [7,16]. We have reported in earlier studies that activated seminal leucocytes affect sperm morphology and sperm motility through the mediation of hydrogen peroxide [23].…”

mentioning

confidence: 95%

See 2 more Smart Citations

Sperm Characteristics and Accessory Sex Gland Functions in Hiv-Infected Men

Umapathy¹,

Simbini²,

Chipata³

et al. 2001

Archives of Andrology

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“…AIDS patients, which could be attributed to the changes in fertility parameters [7,16], an observation confirmed in the present study.…”

supporting

confidence: 89%

Section: E Umapathy Et Almentioning

confidence: 99%

See 1 more Smart Citation

Sperm Characteristics and Accessory Sex Gland Functions in Hiv-Infected Men

Umapathy¹,

Simbini²,

Chipata³

et al. 2001

Archives of Andrology

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“…However, a study of HIV positive men showed that HIV per se did not significantly reduce the sperm count, although there was a significant reduction in sperm motility. AZT had no significant effect on the sperm count or motility (Crittenden et al, 1992).…”

Section: Infectionsmentioning

confidence: 75%

Gonadal function in men with chronic illness

Turner

Wass

1997

Clinical Endocrinology

104

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“…Hyper-viscous semen samples could therefore be treated to reduce viscosity [28]. This is of importance especially in patient populations with high incidences of sexually transmitted infections such as HIV [29] and Ureaplasma urealyticum [30,31], with increased prevalence of seminal hyper-viscosity. The addition of trypsin to semen has been proven to effectively reduce seminal viscosity [32,33], whereby sperm yield during processing could also be improved.…”

Section: Discussionmentioning

confidence: 99%

Treatment of human sperm with serine protease during density gradient centrifugation

Fourie

Loskutoff²,

Huyser

2012

J Assist Reprod Genet

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Purpose Seminal pathogens can bind specifically or nonspecifically to spermatozoa, rendering semen decontamination procedures ineffective, whereby vertical or horizontal transmission of the infection could occur. Serine proteases have been demonstrated to effectively inactivate viruses and to break pathogen-sperm bonds. However, the addition of a protease to density gradient layers during semen processing could negatively impact on sperm parameters. This study investigated the effect of the addition of a recombinant, human-sequence protease (rhProtease) on sperm parameters during density gradient centrifugation. Methods (i) Pooled semen samples (n09) were split and processed by density gradient centrifugation, with the top density layers supplemented, or non-supplemented with rhProtease at three different concentrations (diluted 2, 10 and 20 times). Sperm parameters were then analysed by flow cytometry and computer-assisted semen analyses.(ii) Semen samples (n05) were split and similarly processed using PureSperm® Pro, with rhProtease in the 40 % density gradient layer, or standard PureSperm® not supplemented with rhProtease (Nidacon, International) respectively. The Hemizona assay was then utilized to compare sperm-zona binding post processing.Results Evaluation of sperm parameters indicated that rhProtease did not, at any of the tested concentrations, have an impact on (i) mitochondrial membrane potential, vitality, motility, or (ii) zona binding potential. Conclusion We report that the addition of rhProtease to density gradients is a non-detrimental approach that could improve the effectiveness of semen processing for the elimination of seminal pathogens, and benefit assisted reproduction outcome.

show abstract

Semen analysis in human immunodeficiency virus infection

Cited by 48 publications

References 15 publications

Sperm Characteristics and Accessory Sex Gland Functions in Hiv-Infected Men

Sperm Characteristics and Accessory Sex Gland Functions in Hiv-Infected Men

Gonadal function in men with chronic illness

Treatment of human sperm with serine protease during density gradient centrifugation

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