Semen Characteristics in German Shepherd Dogs

Shalini, I.; Antoine, D.

doi:10.20546/ijcmas.2018.703.270

Cited by 4 publications

(13 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our study, the initial progressive motility rate was 89 %. This finding was similar to that of (Gunay et al, 2003), who reported 84.42±5.3 % initial motility of the first ejaculate, and Shalini & Antoine (2018), who reported 83.3±0.79 % initial motility. (Ray et al, 2019) reported a lower (76.333±2.728 % progressive motility) than the above values.…”

Section: -Discussionsupporting

confidence: 92%

“…Before conducting our experiment, initial quality parameters of fresh semen were measured immediately after collection and pooling. The average ejaculate volume in our study was 8ml, which was like the result obtained by Shalini and Antoine (2018).…”

Section: -Discussionsupporting

confidence: 90%

“…(Ray et al, 2019) reported a lower (76.333±2.728 % progressive motility) than the above values. The estimated acrosome integrity (93%) in this study is higher than that obtained by Shalini & Antoine, (2018) and Umamageswari et al, (2015). In fresh dog sperm, they found 76.83± 2.63 % and 79.0± 0.83 % acrosome integrity, respectively.…”

Section: -Discussioncontrasting

confidence: 82%

“…Percentage of positive HOST recorded in our work is somewhat lower than that recorded in the study of (A Rota et al, 1995) which was 93.6%. The total sperm abnormality was 9% however; it was 7-10%, 14.830 ± 2.386 and 7.25 ± 0.86% in data recorded by Kurien et al, (2012), Ray et al, (2019), andShalini &Antoine, (2018), respectively.…”

Section: -Discussionmentioning

confidence: 71%

See 3 more Smart Citations

Effect of tris-citric acid-egg yolk-glycerol and vitrified medium with and without ascorbic acid on the quality of post- thaw canine semen

Elbehiry

Abdelkéfi²,

Hattab³

et al. 2023

Damanhour Journal of Veterinary Sciences

View full text Add to dashboard Cite

The study aimed to compare vitrified medium (Soya lecithin 1% and 0.25 M sucrose) with tris-citric acid fructose-egg yolk 20%-glycerol 3% (TCF-EY-GL) for freezing of canine semen and assess the impact of adding ascorbic acid to both extenders on the post-thawing semen quality. Semen was collected from 3 healthy and fertile German shepherd dogs by digital manipulation and stimulation of the bulbus glandis. Collected semen was initially evaluated by a computerized assisted semen analyzer (CASA). Ejaculates from the dogs were pooled and divided into 4 equal parts and diluted 1:1 with the following 4 extenders: TCF-EY-GL with ascorbic acid (Ex1), TCF-EY-GL without ascorbic acid (Ex2), vitrified medium with ascorbic acid (Ex3) and vitrified medium without ascorbic acid (Ex4). The extended semen allowed for equilibrating for 1 h in a refrigerator set at 4 ºC then packed into 0.5ml straws. Straws containing semen diluted in Ex1 and2 were frozen by the conventional method however, those containing semen diluted in Ex3 and Ex4 (vitrified media) were plunged directly in liquid nitrogen. Semen samples were evaluated by CASA immediately after thawing. Results revealed that progressive motility, normal morphology, and acrosome integrity were significantly higher in vitrified medium (Soya lecithin 1% and 0.25 M sucrose) than those obtained in TCF-EY-glycerol however, plasma membrane integrity was significantly improved in TCF-EY-GL compared with that observed in the vitrified medium. Addition of ascorbic acids to vitrified medium and TCF-EY-GL resulted in a significant increase in all semen quality parameters. Total sperm motility, progressive motility, and normal sperm morphology were significantly higher in vitrified medium with ascorbic acid than those observed in TCF-EY-GL with ascorbic acid. It was concluded that the addition of ascorbic acid to vitrified medium or TCF-EY-GL extender resulted in significant improvement in postthawing canine semen quality and the vitrified medium can be used as a good alternative for TCF-EY-GL extender to facilitate freezing and avoid undesirable use of EY.

show abstract

Section: -Discussionsupporting

confidence: 92%

Section: -Discussionsupporting

confidence: 90%

Section: -Discussioncontrasting

confidence: 82%

Section: -Discussionmentioning

confidence: 71%

See 2 more Smart Citations

Effect of tris-citric acid-egg yolk-glycerol and vitrified medium with and without ascorbic acid on the quality of post- thaw canine semen

Elbehiry

Abdelkéfi²,

Hattab³

et al. 2023

Damanhour Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…El proceso de colecta de semen utilizando la metodología de mano enguantada (2) logró la obtención del semen a partir del estímulo manual en todos los individuos, adicionalmente en todas las colectas se evidenció una apariencia del semen blanquecino lechoso, esto concuerda con lo reportado por Olivera y Gobello (2), Robert et al (4), Zorinkimi et al (12), Zorinkimi et al (13) y Shalini et al (14).…”

Section: Discussionunclassified

Criopreservación de semen canino (Canis familiaris) en pajilla francesa en el municipio de Medellín, Antioquia

Arango-Múnera

Castrillón-Zuluaga

Correa-Rendón

et al. 2020

Rev Colombiana Cienc Anim. RECIA

View full text Add to dashboard Cite

El objetivo de este trabajo fue aplicar la metodología de criopreservación de semen canino en pajilla francesa en el municipio de Medellín- Antioquia. Se realizó una colecta seminal a ocho caninos adultos, por método de mano enguantada, cada eyaculado fue evaluado macroscópica y microscópicamente y posteriormente diluido con Triladyl® a una concentración final de 80 x 106 espermatozoides/ml. Se empacaron en pajillas de 0,5 ml y fueron sometidas a criopreservación con un descenso de temperatura rápido, sometiendo las pajillas a vapores de nitrógeno durante 25 minutos y luego transferidas directamente a nitrógeno líquido (método de pajilla francesa). En el semen fresco, se observó una apariencia blanquecina lechosa, un volumen de 2.27 ml (±1.40) una concentración de 388.5 x106 espermatozoides/ mL (±228.069), una motilidad individual de 79% (± 4%) y un vigor de 3.96 (±0.327). Se obtuvieron 123 pajillas, de las cuales se tomaron 27 aleatoriamente y se les evaluó la motilidad individual (51 ± 19%) y vigor (2.89 ± 1.02). En conclusión, la aplicación de la metodología de criopreservación rápida por pajilla francesa, usando Triladyl® como crioprotector, se sugiere como protocolo eficiente para la criopreservación de semen canino en el municipio de Medellín.

show abstract