Semen deposition by cervical, transcervical and intrauterine route for fixed-time artificial insemination (FTAI) in the ewe

Casali, R.; Pinczak, A.; Cuadro, F.; Guillen-Muñoz, J.M.; Mezzalira, Alceu; Menchaca, A.

doi:10.1016/j.theriogenology.2017.07.021

Cited by 39 publications

(32 citation statements)

References 24 publications

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“…In fact, our data indicate a mean insemination time of 2.18–2.93 min (depending on the surgical table), which is similar to that described by Casali et al. () (2.98 min).…”

Section: Laparoscopic Intrauterine Insemination: the Shortest Road Fosupporting

confidence: 91%

“…The short insemination times rarely occur since they require the ideal conditions for insemination (immobile ewe, accessibility of the cervix, etc.). On the other hand, the performance (inseminated ewe/hour) of the CAI method is much higher than those of the TCAI or LAI (85.5 vs. 11.4 vs. 56.8, respectively; Casali et al., ).…”

Section: Cervical Insemination: a Classic To Improvementioning

confidence: 98%

“…The fertility (frozen‐thawed semen) is variable: 45% in 38 ewes (Halbert et al., ); 26% in 264 ewes (Windsor et al., ); and 32.5% in 1,809 ewes (Buckrell et al., ). The main problem lies in the percentage of ewes in which the cervix is not crossed, as well as the time that is invested in the penetration 2 min (Windsor et al., ); 3.6 min (Buckrell et al., ); 4.7 min (Husein et al., ); and 5.2 min (Casali et al., ). When the TCAI time exceeds two min, the technique stops being attractive, and the LAI becomes more favourable because it is faster and has better results.…”

Section: Transcervical Intrauterine Insemination: a Challenge Mid‐waymentioning

confidence: 99%

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Current challenges in sheep artificial insemination: A particular insight

Álvarez

Anel-López

Boixo

et al. 2019

Reprod Domestic Animals

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Ovine artificial insemination (OAI) is not commonly performed because of specific problems related to semen application techniques, leading to highly variable results.The ideal methodology (frozen-thawed semen/vaginal route) is unfeasible under field conditions due to the cervix morphology of the ewe, which prevents the process of intrauterine insemination necessary to obtain acceptable results. Currently, OAI commercial programmes use superficial cervical insemination, CAI (vaginal), with chilled semen (15°C) and intrauterine insemination, LAI (laparoscopic), with frozen-thawed semen. The ability to improve upon these contrasting techniques may be derived from examining certain poorly studied factors such as insemination time, productive state of females and alternatives of seminal preservation, some of which we reviewed in this work. This interim solution will remain in use until AI by the vaginal route with frozen-thawed semen is developed, but it poses new challenges in optimizing the freezing of the sperm and adapting the cervical (CAI) and/or transcervical intrauterine AI (TCAI). In this review, we address the current problems and evaluate their methodological (mechanical) and chemical (dilation) alternatives. Currently, TCAI is a methodologically complex technique with poor fertility results, so further studies are needed to improve the logistics of this procedure and the results of its application. K E Y W O R D SAI, cervix, cryopreservation, ovine, sperm, transcervical | 33 ALVAREZ Et AL.

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“…In fact, our data indicate a mean insemination time of 2.18–2.93 min (depending on the surgical table), which is similar to that described by Casali et al. () (2.98 min).…”

Section: Laparoscopic Intrauterine Insemination: the Shortest Road Fosupporting

confidence: 91%

Section: Cervical Insemination: a Classic To Improvementioning

confidence: 98%

Section: Transcervical Intrauterine Insemination: a Challenge Mid‐waymentioning

confidence: 99%

See 1 more Smart Citation

Current challenges in sheep artificial insemination: A particular insight

Álvarez

Anel-López

Boixo

et al. 2019

Reprod Domestic Animals

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show abstract

“…Cervical injury, abscesses, infections are also associated with the transcervical technique (Cseh et al, 2012). In addition, more time per inseminated ewe is required when compared to laparoscopy (~11 vs. ~57 ewes FTAI per hour for transcervical and laparoscopic insemination, respectively) (Casali et al, 2017). In contrast, the laparoscopic technique allows the direct transfer of semen in the lumen of the uterus, achieving 60-80% pregnancy rates (Parkinson, 2009), with seminal doses of 40x10 6 (minimum) total spermatozoa (Cseh et al, 2012).…”

Section: Frozen Semenmentioning

confidence: 99%

Technical recommendations for artificial insemination in sheep

et al. 2019

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Artificial insemination (AI) was the first important biotechnology applied to improve the genetics of farm animals. It allows the rapid and massive diffusion of desirable characteristics of males with high productive potential. We describe the different types of estrus induction and synchronization techniques and the use of the AI with fresh, chilled or frozen semen. Through the adequacy of the protocols of estrus synchronization and AI to the different production systems, the efficient use of reproductive techniques is possible, reaching acceptable pregnancy rates. Summary of reproductive results obtained using cervical and laparoscopic AI are presented.

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“…AI is a more economical, simpler, and a more successful technique than embryo transfer or natural mating for the rapid dissemination of elite livestock genetics [1]. Semen preservation which is a the key step in AI, prolongs storage time and maintains fertilization capacity, facilitates long-distance transportation, and improves the reproductive efficiency of elite male animals [2,3]. One of two sperm preservation protocols, liquid or frozen, may be employed for AI [4].…”

Section: Introductionmentioning

confidence: 99%

Grape Seed Procyanidin Extract (GSPE) Improves Goat Sperm Quality When Preserved at 4 °C

Wen

Feng

et al. 2019

Animals

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Simple SummaryArtificial insemination (AI) is widely used in goats, stimulating the development of semen preservation techniques. Oxidative stress is considered to be the main cause of sperm quality decline over time. In this study, we explored the effect of grape seed procyanidin extract (GSPE) during the liquid preservation of goat semen. The results showed that adding GSPE into the basic diluent enhanced sperm quality by eliminating oxidative stress. The most suitable concentration for the preservation of goat semen at 4 °C was 30 mg/L. This work suggests that promotes the viability of goat semen stored at low temperatures and provides a theoretical foundation for the development of more efficient diluents.AbstractGrape seed procyanidin extract (GSPE) has been shown to possess antioxidative effects. This experiment was designed to study the effect of GSPE during the liquid storage of goat semen. Semen samples were collected from six sexually mature goats. The samples were treated with different concentrations of GSPE (10, 30, 50, and 70 mg/L) in basic diluent and stored at 4 °C for 120 h; samples without GSPE were used as the control group. The results showed that sperm motility, acrosome membrane integrity, mitochondrial activity, plasma membrane integrity, total antioxidative capacity (T-AOC), catalase (CAT) activity, and superoxide dismutase (SOD) activity in the treatment groups were significantly higher than in the control group, whereas malondialdehyde (MDA) content was lower than in the control group (p < 0.05). In the treatment group, sperm quality in the 30 mg/L GSPE group was significantly higher than the other groups (p < 0.05). Furthermore, artificial insemination (AI) results showed that litter sizes were higher in the 30 mg/L GSPE group than in the control group (p < 0.05). In summary, this experiment showed that adding GSPE to the basic diluent improved sperm quality and that 30 mg/L of GSPE was the most suitable concentration for the liquid preservation of goat semen at 4 °C.

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Semen deposition by cervical, transcervical and intrauterine route for fixed-time artificial insemination (FTAI) in the ewe

Cited by 39 publications

References 24 publications

Current challenges in sheep artificial insemination: A particular insight

Current challenges in sheep artificial insemination: A particular insight

Technical recommendations for artificial insemination in sheep

Grape Seed Procyanidin Extract (GSPE) Improves Goat Sperm Quality When Preserved at 4 °C

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