Semen preparation techniques for intrauterine insemination

Boomsma, Carolien M.; Cohlen, Ben J; Farquhar, Cindy

doi:10.1002/14651858.cd004507.pub4

Cited by 38 publications

(33 citation statements)

References 73 publications

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“…In this study two different techniques with the use of sperm preparation procedures (Fakhrildin, et al [21] ). The present study viewed a highly significant species that badly influenced the fertilization ability (Boomsma, et al [23] ).…”

Section: Resultsmentioning

confidence: 99%

Comparing the Activation Role of Centrifugation Swim-up and the Density Gradient Combined with ALLGrad Sperm Preparation Techniques

Muhammad¹,

Fakhrildin²

2019

IJEIR

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In about 40% of infertile couples, the husband was found to be either the single or a contributing cause of infertility and the systemic management ready for those couples with male infertility are restricted. 135 couples with infertility shared herein; the husbands required in vitro activation for their sperm samples, their wives however; had no fertility problem and were submitted ovarian induction protocol. However; Sperm preparation was done using both, the centrifugation indirect swim-up technique with Global medium and density gradient with ALLGrad solution. While ALLGrad 45% is a ready-to-be-used upper layer. There was an acceleration in seminal parameters after preparation using both various techniques. So, a highly significant increment (P≤0.001) in the percentages of progressive forward motility was in grades (A and B) and grade (A) progressive sperm motility, also a significant enhancement (P≤0.05) in percentage of morphologically normal sperms when applying density gradient mechanism in relation to centrifugation swim-up technique. This study concludes that the use of density gradient mechanism with ALLGrad solution and Global culture medium are more efficient in achieving better percentages of progressive forward motility grades (A and B), grade (A) progressive motility and morphologically normal values of spermatozoa than centrifugation indirect swim-up technique by using Global culture medium. However, it was found there was a non-significant difference when we use both techniques concerning other sperm parameters.

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Section: Resultsmentioning

confidence: 99%

Comparing the Activation Role of Centrifugation Swim-up and the Density Gradient Combined with ALLGrad Sperm Preparation Techniques

Muhammad¹,

Fakhrildin²

2019

IJEIR

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“…4 Many sperm preparation procedures are available, but there are three main groups of methods. 9 First method is by selection of spermatozoa on their ability to swim, known as the "swim-up technique". This is performed by layering culture medium over the liquefied semen.…”

Section: Resultsmentioning

confidence: 99%

“…Sperm preparation by the discontinuous gradient technique provides good yield of spermatozoa from ejaculates with very low sperm density eliminates leucocytes and significantly reduces reactive oxygen species thereby recovers a clean fraction of highly motile spermatozoa. 11 While there are several studies claiming effectiveness of one method over the other, a recent systematic review 9 conclude that there is no clear evidence of benefit of gradient technique over the Swim-up method. The outcomes studied, however, were firstly those after IUI; secondly, they were mainly on recovery of optimum concentration of good quality of spermatozoa.…”

Section: Resultsmentioning

confidence: 99%

“…The pellet formed at the bottom part after centrifugation is resuspended in a small quantity of medium and incubated until the time of insemination. 9 When the conventional wash can be done twice, it is called "double wash swim-up technique. "…”

Section: Introductionmentioning

confidence: 99%

“…The Cochrane systematic review on semen preparation techniques for intrauterine insemination (2007) conclude that there is insufficient data from RCTs to recommend any of the three semen preparation techniques. 9 There is no clear evidence of benefit between one method over the other and hence recommended more quality research. This systematic review is on outcomes following IUI.…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of Double Wash Swim-Up Versus Double Density Gradient Swim-Up Technique of Sperm Preparation in in Vitro Fertilisation

Sangisapu¹,

Karunakaran²,

Pillai³

2017

jebmh

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BACKGROUND Recovery of optimum number of good quality of spermatozoa is an important component of In Vitro Fertilisation (IVF). This is achieved by sperm preparation methods involving separation and recovery of capacitated sperms. Double Wash Swim-up (DWSU) and Double Density Gradient Swim-up (DDGSU) are two most accepted methods. Cochrane systematic review (2007) finds no clear benefit of one method over the other in Intrauterine Insemination (IUI). Systematic review on effectiveness of these preparations in IVF is lacking. Effectiveness is generally assessed in terms recovery rates of the sperms. Capability of successful fertilisation of good quality oocytes should ideally be the functional endpoint for evaluating effectiveness of sperm preparation methods. The aim of the study is to-1. Compare the successful fertilisation rates of oocytes inseminated by semen preparation of Double Wash Swim-up (DWSU) visa -vis by Double Density Gradient Swim-up (DDGSU) method. 2. Evaluate the effectiveness of fertilisation of oocytes by Double Wash Swim-up method (DWSU) visa -vis Double Density Gradient Swim-up (DDGSU) method.

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Double versus single intrauterine insemination (IUI) in stimulated cycles for subfertile couples

Rakic

Kostova

Cohlen

et al. 2021

Cochrane Database of Systematic Reviews

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Semen preparation techniques for intrauterine insemination

Cited by 38 publications

References 73 publications

Comparing the Activation Role of Centrifugation Swim-up and the Density Gradient Combined with ALLGrad Sperm Preparation Techniques

Comparing the Activation Role of Centrifugation Swim-up and the Density Gradient Combined with ALLGrad Sperm Preparation Techniques

Effectiveness of Double Wash Swim-Up Versus Double Density Gradient Swim-Up Technique of Sperm Preparation in in Vitro Fertilisation

Double versus single intrauterine insemination (IUI) in stimulated cycles for subfertile couples

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