Semiconducting Polymer Nanobioconjugates for Targeted Photothermal Activation of Neurons

Lyu, Yan; Xie, Chen; Chechetka, Svetlana A.; Miyako, Eijiro; Pu, Kanyi

doi:10.1021/jacs.6b05192

Cited by 423 publications

(421 citation statements)

References 30 publications

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“…In summary, these results indicate that LM nanocapsules can also act as nanostimulators for the remote control of ion channels using an external light source. We believe that one of the advantages of LM nanocapsules in comparison with other light-driven cellular stimulation62 including our previous research applied semiconducting polymer nanoparticles71 is the possibility to be excited at various wavelength of light because LMs have absorbance over a wide range in the optical region (Supplementary Fig. 22).…”

Section: Resultsmentioning

confidence: 99%

Light-driven liquid metal nanotransformers for biomedical theranostics

et al. 2017

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Room temperature liquid metals (LMs) represent a class of emerging multifunctional materials with attractive novel properties. Here, we show that photopolymerized LMs present a unique nanoscale capsule structure characterized by high water dispersibility and low toxicity. We also demonstrate that the LM nanocapsule generates heat and reactive oxygen species under biologically neutral near-infrared (NIR) laser irradiation. Concomitantly, NIR laser exposure induces a transformation in LM shape, destruction of the nanocapsules, contactless controlled release of the loaded drugs, optical manipulations of a microfluidic blood vessel model and spatiotemporal targeted marking for X-ray-enhanced imaging in biological organs and a living mouse. By exploiting the physicochemical properties of LMs, we achieve effective cancer cell elimination and control of intercellular calcium ion flux. In addition, LMs display a photoacoustic effect in living animals during NIR laser treatment, making this system a powerful tool for bioimaging.

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Section: Resultsmentioning

confidence: 99%

Light-driven liquid metal nanotransformers for biomedical theranostics

et al. 2017

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“…SPNs constitute not only a new generation of organic nanoparticles for sensing and imaging139, 140, 141, 142, 143 but also a noninvasive remote control method with a near‐infrared absorbing property to regulate in vivo gene expression and cellular signals 135, 142, 144. What is particularly exciting is their potential in optimized cancer therapy 145, 146.…”

Section: Discussionmentioning

confidence: 99%

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

2018

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Extracellular vesicles (EVs) are ubiquitous nanosized membrane vesicles consisting of a lipid bilayer enclosing proteins and nucleic acids, which are active in intercellular communications. EVs are increasingly seen as a vital component of many biological functions that were once considered to require the direct participation of stem cells. Consequently, transplantation of EVs is gradually becoming considered an alternative to stem cell transplantation due to their significant advantages, including their relatively low probability of neoplastic transformation and abnormal differentiation. However, as research has progressed, it is realized that EVs derived from native‐source cells may have various shortcomings, which can be corrected by modification and optimization. To date, attempts are made to modify or improve almost all the components of EVs, including the lipid bilayer, proteins, and nucleic acids, launching a new era of modularized EV therapy through the “modular design” of EV components. One high‐yield technique, generating EV mimetic nanovesicles, will help to make industrial production of modularized EVs a reality. These modularized EVs have highly customized “modular design” components related to biological function and targeted delivery and are proposed as a promising approach to achieve personalized and precision medicine.

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“…Among carbon-based materials, carbon nanotubes, graphene and polymer nano-bioconjugates can also sustain photo-thermal stimulation (Miyako et al, 2014; Chechetka et al, 2016; Lyu et al, 2016; Li et al, 2017). Yet, there is a fundamental difference to be taken into account when comparing those systems with our P3HT NPs: for both inorganic- and organic-based systems reported so far, NIR-mediated photo-thermal heating is the main phenomenon, indirectly leading to ROS enhancement.…”

Section: Discussionmentioning

confidence: 99%

“…Several attempts have been reported to modulate Ca 2+ by external mediators, for instance by using magnetic NPs (Huang et al, 2010; Stanley et al, 2012; Bar-Shir et al, 2014; Lee et al, 2014; Chen et al, 2015; Wheeler et al, 2016), metallic NPs (Nakatsuji et al, 2015), organic molecules and polymers (Stein et al, 2013; Lyu et al, 2016; Takano et al, 2016) and other carbon-based materials (Miyako et al, 2014; Chechetka et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells

Bossio

Aziz

Tullii

et al. 2018

Front. Bioeng. Biotechnol.

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Optical modulation of living cells activity by light-absorbing exogenous materials is gaining increasing interest, due to the possibility both to achieve high spatial and temporal resolution with a minimally invasive and reversible technique and to avoid the need of viral transfection with light-sensitive proteins. In this context, conjugated polymers represent ideal candidates for photo-transduction, due to their excellent optoelectronic and biocompatibility properties. In this work, we demonstrate that organic polymer nanoparticles, based on poly(3-hexylthiophene) conjugated polymer, establish a functional interaction with an in vitro cell model (Human Embryonic Kidney cells, HEK-293). They display photocatalytic activity in aqueous environment and, once internalized within the cell cytosol, efficiently generate reactive oxygen species (ROS) upon visible light excitation, without affecting cell viability. Interestingly, light-activated ROS generation deterministically triggers modulation of intracellular calcium ion flux, successfully controlled at the single cell level. In perspective, the capability of polymer NPs to produce ROS and to modulate Ca2+ dynamics by illumination on-demand, at non-toxic levels, may open the path to the study of biological processes with a gene-less approach and unprecedented spatio-temporal resolution, as well as to the development of new biotechnology tools for cell optical modulation.

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Semiconducting Polymer Nanobioconjugates for Targeted Photothermal Activation of Neurons

Cited by 423 publications

References 30 publications

Light-driven liquid metal nanotransformers for biomedical theranostics

Light-driven liquid metal nanotransformers for biomedical theranostics

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells

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